We narrowed to 1,028 results for: Psp

1. pCPmacroH2A.1-GS3

   Plasmid

   #204759

   Purpose

   Expression of Cas9 and human macroH2A.1

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   macroH2A1 (MACROH2A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Cas9-GFP_sg_mTfeb

   Plasmid

   #79006

   Purpose

   Expresses WT Cas9 and GFP, along with a sgRNA to mouse Tfeb.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   sgTfeb (Tfeb Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. PX459-TP53-exon4

   Plasmid

   #217455

   Purpose

   For TP53 knockout targeting exon 4 of TP53

   Depositor

   John Diffley

   Article

   Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

   Insert

   CRISPR Cas9 guide for TP53 (TP53 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. PX459-TP53-exon5

   Plasmid

   #217456

   Purpose

   For TP53 knockout targeting exon 5 of TP53

   Depositor

   John Diffley

   Article

   Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

   Insert

   CRISPR Cas9 guide for TP53 (TP53 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCPH2A.X_S139A-GS3

   Plasmid

   #204749

   Purpose

   Expression of Cas9 and human H2AX with the S139A mutation

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2AX S139A (H2AX Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H2AX with the S139A mutation

   Promoter

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. SaCas9v2-Puro

   Plasmid

   #178802

   Purpose

   SaCas9 with 2A-Puro, and a cloning backbone for sgRNA.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh (Cas9-2A Puro) and U6 (gRNA)

   Available Since

   Aug. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCPH2A.X_S139D-GS3

   Plasmid

   #204748

   Purpose

   Expression of Cas9 and human H2AX with the S139D mutation

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2AX S139D (H2AX Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H2AX with the S139D mutation

   Promoter

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. SOX17-3'gRNA

   Plasmid

   #210465

   Purpose

   gRNA targeting SOX17 3' terminal for CRISPR-Cas9-mediated knock-in

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Insert

   SOX17 (SOX17 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPBC-LG3-tdT

   Plasmid

   #62810

   Purpose

   pPBC-LG3-tdT expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP3 and tdTomato under the control of the CAG promoter.

   Depositor

   Karen Wilcox

   Article

   Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

   Insert

   ITR-CAG-Lck-GCaMP3-IRES-tdTomato-ITR
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. His-GST-Cys-M87 Spastin

    Plasmid

    #184804

    Purpose

    Bacterial expression of M87 Spastin (A2C mutation)

    Depositor

    James Hurley

    Article

    Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

    Insert

    M87 Spastin (SPAST Human)
    

    Use

    Tags

    His6-GST

    Expression

    Bacterial

    Mutation

    A2C

    Promoter

    T7

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiGuideFB-Puro-A

    Plasmid

    #192506

    Purpose

    For cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a nd a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI.

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

    Insert

    hU6-sgRNA-CS1-BsmBI-EFS-Puro-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available Since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiGuideFB-Puro-B

    Plasmid

    #192507

    Purpose

    For cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI.

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

    Insert

    bU6-sgRNACR2-CS-BsmBI-EFS-Puro-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    bU6

    Available Since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. mApple-M1 SpastinHFDD

    Plasmid

    #134462

    Purpose

    M1 SpastinHFDD expression

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

    Insert

    M1 Spastin (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Met to Ala at amino acid 87; His to Asp at amino …

    Promoter

    CMV

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. P63-sp64 Poly A

    Plasmid

    #59435

    Purpose

    synthetic poly A transcripts of VZV

    Depositor

    Randall Cohrs

    Article

    Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

    Insert

    VZV ORF 63
    

    Use

    Makes synthetic transcripts that contains poly a

    Tags

    none

    Expression

    Mutation

    none

    Promoter

    SP6

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

15. P29-sp64 Poly A

    Plasmid

    #59434

    Purpose

    synthetic poly A transcripts of VZV

    Depositor

    Randall Cohrs

    Article

    Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

    Insert

    VZV ORF 29
    

    Use

    Makes synthetic transcripts that contains poly a

    Tags

    none

    Expression

    Mutation

    none

    Promoter

    SP6

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

16. Mouse c-myc (exons II and III)

    Plasmid

    #8448

    Depositor

    Frederic Mushinski

    Article

    Weissinger et al Mol Cell Biol 1993 Apr;13(4):2578-85.

    Insert

    Mouse c-myc (exons II and III) (Myc Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Exons II and III only

    Promoter

    Available Since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

17. P21-sp64 Poly A

    Plasmid

    #59433

    Purpose

    synthetic poly A transcripts of VZV

    Depositor

    Randall Cohrs

    Article

    Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

    Insert

    VZV ORF 21
    

    Use

    Makes synthetic transcripts that contains poly a

    Tags

    none

    Expression

    Mutation

    none

    Promoter

    SP6

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

18. Rev CDK9 (D167N) (P#858)

    Plasmid

    #14635

    Depositor

    Matija Peterlin

    Article

    Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

    Insert

    cdk9 D167N (CDK9 Human)
    

    Use

    Tags

    Rev

    Expression

    Mammalian

    Mutation

    D167N. Kinase negative mutant.

    Promoter

    Available Since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pCJ1023

    Plasmid

    #228763

    Purpose

    Plasmid expressing Cas9 and gRNAs for mouse Ift88 and Pkd2. Use for disruption of mouse Ift88 and Pkd2 in cultured cells.

    Depositor

    Bradley Yoder

    Insert

    Ift88 and Pkd2 gRNAs (Ift88, Pkd2 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. ABEmax-Puro V2

    Plasmid

    #226955

    Purpose

    ABEmax plasmid enabling A:T to G:C base editing using a single plasmid.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge