We narrowed to 1,145 results for: cas12

1. pC0060 AspCas13 (B04) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115210

   Purpose

   Expresses AspCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   AspCas13
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC0063 PauCas13 (B07) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115214

   Purpose

   Expresses PauCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   PauCas13
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MCP-M-MLV RT∆RNase H

   Plasmid

   #213749

   Purpose

   For circular RNA-mediated prime editor using MCP-M-MLV RT∆RNase H in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   MCP-M-MLV RT∆RNase H
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-21a_FnCpf1_2xNLS_protein_expression

   Plasmid

   #124548

   Purpose

   Protein expression plasmid for 2xNLS FnCpf1 in pET-21a backbone

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   FnCpf1_2xNLS
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRosetta_v2

   Plasmid

   #136477

   Purpose

   Lentiviral GFP Reporter assay for determining appropriate dose of puromycin, blasticidin, and hygromycin

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

   Insert

   HygroR-T2A-BlastR-P2A-PuroR-F2A-EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   PGK

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEL031

   Plasmid

   #137900

   Purpose

   STU2.0 SpyCas9 tRNA system for plant genome editing

   Depositor

   Yong Zhang

   Article

   Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pC0062 RanCas13 (B06) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115213

   Purpose

   Expresses RanCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   RanCas13
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pYPQ166

   Plasmid

   #109328

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter

   Depositor

   Yiping Qi

   Article

   Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

   Insert

   zCas9
   

   Use

   CRISPR; Gateway compatible zcas9 entry clone

   Tags

   3x FLAG, NLS and NLS

   Expression

   Plant

   Mutation

   Zea mays codon-optimized Cas9

   Promoter

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC0067 PguCas13 (B11) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115218

   Purpose

   Expresses PguCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   PguCas13
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. U6-5' MS2-CircRNA

    Plasmid

    #213750

    Purpose

    For circular RNA-mediated prime editor using U6-5' MS2-CircRNA in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    5' ribozyme, 5' ligation sequences, 5' MS2, 3' ligation sequences, 3' ribozyme
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pN256

    Plasmid

    #188472

    Purpose

    scDAISY-seq lentiviral construct encoding crRNA targeting the DAISY chain that is within the UTR of eGFP

    Depositor

    Le Cong

    Article

    Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

    Insert

    eGFP-WPRE-DAISY-4T
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. U6-3' MS2-CircRNA

    Plasmid

    #213751

    Purpose

    For circular RNA-mediated prime editor using U6-3' MS2-CircRNA in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    5' ribozyme, 5' ligation sequences, 3' MS2, 3' ligation sequences, 3' ribozyme
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFnCpf1GG

    Plasmid

    #131010

    Purpose

    Backbone plasmid for generating CRISPR arrays for FnCas12a. Contains a GFP-dropout cassette and a direct repeat.

    Depositor

    Chase Beisel

    Article

    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

    Inserts

    direct repeat of FnCas12a
    GFP expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMV_AA090

    Plasmid

    #216099

    Purpose

    Knockout, EF1a-driven Cas12a (Cas only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas12a [EnAs]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUDE1084

    Plasmid

    #168500

    Purpose

    expression of T7RNA polymerase and CRISPR endonunclease Fncas12a

    Depositor

    Jean-Marc Daran

    Article

    Randazzo et al CRISPR J. 2021 Apr 23. doi: 10.1089/cris

    Inserts

    Fncas12a
    T7RNA polymerase
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    TDH3 and TEF1

    Available since

    Dec. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. crTet in pSLQ8453

    Plasmid

    #183965

    Purpose

    U6-crTet-EF1a-Puro-T2A-BFP-WPRE

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Insert

    crTet
    

    Use

    Lentiviral

    Tags

    EF1a-Puro-T2A-BFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEL030

    Plasmid

    #137899

    Purpose

    STU2.0 SpyCas9 Csy4 system for plant genome editing

    Depositor

    Yong Zhang

    Article

    Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEL029

    Plasmid

    #137898

    Purpose

    STU2.0 SpyCas9 RZ system for plant genome editing

    Depositor

    Yong Zhang

    Article

    Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEL033

    Plasmid

    #137902

    Purpose

    STU2.0 rAPOBEC1-SpyCas9(D10A) for plant genome base editing

    Depositor

    Yong Zhang

    Article

    Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEPOR1CB0013

    Plasmid

    #117549

    Purpose

    Level 1 Golden Gate Cassette: LbCas12a expression cassette for dicotyledonous plants

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    2x35S+5'UTR OMEGA (pICH51288) + LbCas12a (with stop codon) (pEPOR0SP0007)+ Nos (pICH41421)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S+5'UTR OMEGA (pICH51288)

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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