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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,083 results for: golden gate

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Showing: 941 - 960 of 7083 results

  1. pOPS0359

    Plasmid
    #115508
    Purpose
    Reporter Plasmid. Taurine-inducible expression of GFP
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    sfGFP pOGG037, T-pharma pOGG003 assembled in pOGG024
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Taurine-inducible promoter of S. meliloti Promote…
    Available Since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    115508-plasmid-map-sequence-id-224277

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  2. pL1P6 ZmUbiP:GRF-GIF:NosT

    Plasmid
    #198049
    Purpose
    MoClo Golden Gate Level 1 Position 6. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.
    Depositor
    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing
    Insert
    ZmUbiP::TaGRF4-GIF1::NosT
    Use
    Synthetic Biology
    Promoter
    Zea mays (maize) ubiquitin promoter
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    198049-plasmid-map-sequence-id-391867

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  3. pL1P4 ZmUbiP:GRF-GIF:NosT

    Plasmid
    #198048
    Purpose
    MoClo Golden Gate Level 1 Position 4. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.
    Depositor
    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing
    Insert
    ZmUbiP::TaGRF4-GIF1::NosT
    Use
    Synthetic Biology
    Promoter
    Zea mays (maize) ubiquitin promoter
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    198048-plasmid-map-sequence-id-389382

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  5. pL1P3 ZmUbiP:GRF-GIF:NosT

    Plasmid
    #198047
    Purpose
    MoClo Golden Gate Level 1 Position 3. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.
    Depositor
    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing
    Insert
    ZmUbiP::TaGRF4-GIF1::NosT
    Use
    Synthetic Biology
    Promoter
    Zea mays (maize) ubiquitin promoter
    Available Since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    198047-plasmid-map-sequence-id-389251

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  6. pL1P5 ZmUbiP:GRF-GIF:NosT

    Plasmid
    #198046
    Purpose
    MoClo Golden Gate Level 1 Position 5. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.
    Depositor
    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing
    Insert
    ZmUbiP::TaGRF4-GIF1::NosT
    Use
    Synthetic Biology
    Promoter
    Zea mays (maize) ubiquitin promoter
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    198046-plasmid-map-sequence-id-383687

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  7. mUAVbla:tetR 5′

    Plasmid
    #149379
    Purpose
    mUAVbla containing a tetracycline resistance (tetA) cassette for building Gene Doctoring donor plasmids from pDOC-GG, using BsaI Golden Gate assembly with GGAG and CGCT overhangs
    Depositor
    Mark Pallen
    Article
    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi
    Insert
    tetA
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149379-plasmid-map-sequence-id-308791

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  9. pOGG014

    Plasmid
    #113988
    Purpose
    Vector construction module. Endlinker/terminator module to circularize plasmid following Part 4
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Endlinker/terminator Part 4
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    113988-plasmid-map-sequence-id-223089

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  10. pOGG015

    Plasmid
    #113989
    Purpose
    Vector construction module. Endlinker/terminator module to circularize plasmid following Part 5
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Endlinker/terminator Part 5
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    113989-plasmid-map-sequence-id-223087

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  11. pOGG013

    Plasmid
    #113987
    Purpose
    Vector construction module. Endlinker/terminator module to circularize plasmid following Part 3
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Endlinker/terminator Part 3
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    113987-plasmid-map-sequence-id-223414

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  12. pOGG016

    Plasmid
    #113990
    Purpose
    Vector construction module. Endlinker/terminator module to circularize plasmid following Part 6
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Endlinker/terminator Part 6
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Sept. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    113990-plasmid-map-sequence-id-223084

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  13. pOGG203

    Plasmid
    #115504
    Purpose
    Clone of a level 1 unit which encodes a constitutively-expressed mCherry
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    pL1M-F2-pNeo pOGG001, mCherry EC15071, T-pharma(pOGG003
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Sept. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    115504-plasmid-map-sequence-id-223070

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  14. pLM433

    Plasmid
    #217293
    Purpose
    For creating CyanoTag vectors by Golden Gate cloning (BspQI version)
    Depositor
    Luke Mackinder
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    mNeonGreen - 3x FLAG
    Available Since
    June 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217293-plasmid-map-sequence-id-435614

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  16. pUCC001

    Plasmid
    #124451
    Purpose
    Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets
    Depositor
    John Morrissey
    Article
    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97
    Insert
    HH-BsaI
    Expression
    Yeast
    Mutation
    gRNA expression cassette from pUDP002 modified to…
    Promoter
    TDH3 promoter from S.cerevisiae (already present …
    Available Since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    124451-plasmid-map-sequence-id-257763

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Showing: 941 - 960 of 7083 results