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We narrowed to 63,900 results for: cloning

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Showing: 9581 - 9600 of 63900 results

  3. pJAT77

    Plasmid
    #204295
    Purpose
    pJAT plasmid with multiple cloning site for introducing custom payload. No U6 promoter. Restriction sites present to introduce flanking U6 promoters.
    Depositor
    David Stern
    Publication
    Stern et al bioRxiv 2023.06.17.545412
    Type
    Empty backbone
    Use
    CRISPR
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204295-plasmid-map-sequence-id-406178

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  6. pSEVA12DKR-J

    Plasmid
    #58306
    Purpose
    GeneGuard genomic cassette: for constructing ΔdapA::RBS J pi, Kis, FRT-kanR-FRT (high-copy support for R6K COR). Will support pSEVAD1K.
    Depositor
    Tom Ellis
    Publication
    Wright et al ACS Synth Biol. 2014 May 13.
    Insert
    Genomic cassette
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available since
    March 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    58306-plasmid-map-sequence-id-89818

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  7. pSEVA12TEC-I

    Plasmid
    #58308
    Purpose
    GeneGuard genomic cassette: for constructing ΔthyA::RBS I repA, Epsilon, FRT-kanR-FRT (medium-copy support for ColE2 COR). Will support pSEVAT7Z.
    Depositor
    Tom Ellis
    Publication
    Wright et al ACS Synth Biol. 2014 May 13.
    Insert
    Genomic cassette
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available since
    March 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    58308-plasmid-map-sequence-id-449692

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  12. pBlastR-MCS-QF-MiniWhite

    Plasmid
    #165906
    Purpose
    Blasticidin resistant QF driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning
    Depositor
    Koen Venken
    Publication
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Insert
    Selectable Empty Enhancer Driver
    Use
    Synthetic Biology
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    165906-plasmid-map-sequence-id-326311

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  13. pG418R-MCS-QF-MiniWhite

    Plasmid
    #165902
    Purpose
    G418 resistant QF driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning
    Depositor
    Koen Venken
    Publication
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Insert
    Selectable Empty Enhancer Driver
    Use
    Synthetic Biology
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    165902-plasmid-map-sequence-id-328281

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  14. pRDA_103

    Plasmid
    #133460
    Purpose
    H1 promoter with two Tet operator (TetO) sites expresses customizable Spyo-guide; short EF1a promoter (EFS) expresses SaurCas9, 2A provides TetR, and 2A provides blasticidin resistance.
    Depositor
    John Doench
    Publication
    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1
    Insert
    control guide
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    133460-plasmid-map-sequence-id-261409

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  15. KA801: pMVP (L3-L2) HA tag + pA; CMV::eGFP-P2A-TETa-pA

    Plasmid
    #121800
    Purpose
    pMVP L3-L2 entry plasmid, contains HA tag-polyA + CMV-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream CMV-driven GFP-P2A-TETa
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    HA epitope tag-polyA + CMV::eGFP-P2A-TETa-polyA
    Use
    Gateway Cloning and Synthetic Biology
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121800-plasmid-map-sequence-id-237308

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  16. KB601: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; EF1a promoter

    Plasmid
    #121819
    Purpose
    pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Gateway Cloning and Synthetic Biology
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121819-plasmid-map-sequence-id-237368

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  17. FB039

    Plasmid
    #119715
    Purpose
    Multipartite assembly obtained by combining FBparts FB026+FB009 into pDGB3omega1. Used for fluorescent tagging of fungi (G418 resistance + YFP), according to FungalBraid modular DNA assembly for ATMT.
    Depositor
    Jose Marcos
    Publication
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    PgpdA::YFP::TtrpC(←)-PtrpC:nptII::Ttub (→)
    Use
    Synthetic Biology
    Available since
    May 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119715-plasmid-map-sequence-id-245416

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  18. FB003

    Plasmid
    #119677
    Purpose
    Transcriptional Unit (TU) for hygromicin resistance in fungi obtained by combining FB001+GB0211+FB002 into pDGB3alpha2. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT
    Depositor
    Jose Marcos
    Publication
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    PtrpC:hph:Ttub
    Use
    Synthetic Biology
    Available since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    119677-plasmid-map-sequence-id-238439

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  19. FB010

    Plasmid
    #119708
    Purpose
    Transcriptional Unit (TU) for hygromicin resistance in fungi obtained by combining FB001+GB0211+FB002 into pDGB3alpha1. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT
    Depositor
    Jose Marcos
    Publication
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    PtrpC:hph:Ttub
    Use
    Synthetic Biology
    Available since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    119708-plasmid-map-sequence-id-236964

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  20. FB001

    Plasmid
    #119057
    Purpose
    Promoter trpC from Aspergillus nidulans domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.
    Depositor
    Jose Marcos
    Publication
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Promoter trpC
    Use
    Synthetic Biology; Domestication of dna parts for…
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119057-plasmid-map-sequence-id-237151

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Showing: 9581 - 9600 of 63900 results