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We narrowed to 60,293 results for: cloning

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Showing: 9581 - 9600 of 60293 results

  4. pHM140Env

    Plasmid
    #139022
    Purpose
    Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)
    Depositor
    Anjali Joshi
    Article
    Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.
    Insert
    HIV-1 Envelope Glycoprotein (env HIV-1)
    Expression
    Mammalian
    Available Since
    April 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    139022-plasmid-map-sequence-id-288124

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  9. pSL1145 (pSPIN, pBBR1 backbone, R*MmeI)

    Plasmid
    #160736
    Purpose
    Single-plasmid V. cholerae CAST, encodes all proteins, crRNA, and donor DNA. Non-targeting crRNA with BsaI sites for spacer cloning. Mini-tn has MmeI site in R end for Tn-seq. pBBR1 backbone.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    160736-plasmid-map-sequence-id-326992

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  11. pcU6_3 MS2 sgRNA

    Plasmid
    #92394
    Purpose
    Chick-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter, with tracrRNA scaffold containing stem loops allowing binding of bacteriophage MS2 coat protein MCP
    Depositor
    Tatjana Sauka-Spengler
    Article
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Insert
    chick U6.3 promoter and gRNA cloning cassette including MS2 stem loops
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    chick U6.3
    Available Since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    92394-plasmid-map-sequence-id-191866

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  12. pLCKO

    Plasmid
    #73311
    Purpose
    Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BfuAI cloning sites and puromycin selection.
    Depositor
    Jason Moffat
    Article
    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Feb. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73311-plasmid-map-sequence-id-134941

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  14. pYPQ141-ZmUbi-RZ-As

    Plasmid
    #86196
    Purpose
    AsCpf1 gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processing
    Depositor
    Yiping Qi
    Article
    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10
    Insert
    AsCpf1 gRNA cloning site for ribozyme cleavage
    Use
    CRISPR
    Tags
    Hammerhead ribozyme and HDV ribozyme
    Expression
    Plant
    Promoter
    Maize ubiquitin 1
    Available Since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    86196-plasmid-map-sequence-id-176061

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Showing: 9581 - 9600 of 60293 results