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Tol2-LyzC-Utrophin-GFP-U6ac-Rac2 guides

Plasmid

#168254

Purpose

"label stable F-actin under Rac2 neutrophil-specific knockout background"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Utrophin-GFP

Use

Zebrafish expression

Promoter

LyzC

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA-SCcpV

Plasmid

#190636

Purpose

Mammalian expression of genetically encoded calcium indicator SCcpV

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

SCcpV

Expression

Mammalian

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-PBD-GFP-U6ac-ctrl guides

Plasmid

#168252

Purpose

"label active Rac; control for neutrophil-specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

PBD GFP

Use

Zebrafish expression

Promoter

LyzC

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-Rac-DN(guide resistant)-2a-mcherry-U6ac-Rac guides

Plasmid

#168243

Purpose

"Dominant negative control of the assay to rescue Rac expression in neutrophil specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Rac(guide resistant)-DN

Use

Zebrafish expression

Tags

mcherry

Promoter

LyzC

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-Rac-CA(guide resistant)-2a-mcherry-U6ac-Rac guides

Plasmid

#168244

Purpose

"Rescue Rac-CA expression in neutrophil specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Rac(guide resistant)-CA

Use

Zebrafish expression

Tags

mcherry

Promoter

LyzC

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-Utrophin-GFP-U6ac-ctrl guides

Plasmid

#168253

Purpose

"label stable F-actin; control for neutrophil-specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Utrophin-GFP

Use

Zebrafish expression

Promoter

LyzC

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLN492 (FuGW-G8p-antiPD1(HC)-1Pe)

Plasmid

#105194

Purpose

Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab heavy chain transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-antiPD1(HC)-1Pe

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLN493 (FuGW-G8p-antiPD1(LC)-1Pe)

Plasmid

#105195

Purpose

Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab light chain transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-antiPD1(LC)-1Pe

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pTF

Plasmid

#162784

Purpose

Protein expression in CHO cells - C-terminal FLAG and 10xhis tags are coded

Depositor

Ivan Vorobiev

Article

Kolesov et al Diagnostics (Basel). 2022 Feb 3;12(2). p

Type

Empty backbone

Tags

10xHis and FLAG

Expression

Mammalian

Promoter

CHO Elongation factor 1A

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pRD400

Plasmid

#163107

Purpose

Expression of eYFP_H-NSdbd in eukaryotes for visualisation of nuclear DNA

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

NLS_eYFP_H-NSdbd_NLS

Use

Eukaryotic expression

Expression

Mammalian

Promoter

CMV promoter; T7 promoter

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRD188

Plasmid

#163099

Purpose

Expression of mEos3.2_H-NSdbd in eukaryotes for visualisation of nuclear DNA

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

NLS_mEos3.2_H-NSdbd_NLS

Use

Eukaryotic expression

Expression

Mammalian

Promoter

CMV promoter; T7 promoter

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRD393

Plasmid

#168774

Purpose

Expression of mEos3.2_H-NSdbd in Mycobacterium

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

mEos3.2_H-NSdbd

Expression

Bacterial

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pEGB_3alpha2 OplacI:mini35S:RDF:Tnos (GB1534)

Plasmid

#160617

Purpose

TU for the regulated expression of the PhiC31 phage recombination directionality factor (RDF) gene driven by a synthetic promoter consisting of the LacI operator fused to the minimal 35S promoter.

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

RDF

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

mini35s

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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mPLD2-PX (62-192)

Plasmid

#119128

Purpose

Bacterial expression of human phox homology (PX) domain, mPLD2-PX (62-192)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

mPLD2-PX (62-192)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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SNX17-PX (1-111)

Plasmid

#119098

Purpose

Bacterial expression of human phox homology (PX) domain, SNX17-PX (1-111)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX17-PX (1-111) (SNX17 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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SNX16-PX (104-216)

Plasmid

#119097

Purpose

Bacterial expression of human phox homology (PX) domain, SNX16-PX (104-216)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX16-PX (104-216) (SNX16 Human)

Promoter

N/A

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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SNX4-PX (48-186)

Plasmid

#119085

Purpose

Bacterial expression of human phox homology (PX) domain, SNX4-PX (48-186)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX4-PX (48-186) (SNX4 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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SNX32-PX (17-166)

Plasmid

#119111

Purpose

Bacterial expression of human phox homology (PX) domain, SNX32-PX (17-166)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX32-PX (17-166) (SNX32 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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SNX7-PX (89-218)

Plasmid

#119088

Purpose

Bacterial expression of human phox homology (PX) domain, SNX7-PX (89-218)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX7-PX (89-218) (SNX7 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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SNX30-PX (86-205)

Plasmid

#119109

Purpose

Bacterial expression of human phox homology (PX) domain, SNX30-PX (86-205)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX30-PX (86-205) (SNX30 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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