We narrowed to 165,899 results for: DES

Showing: 9601 - 9620 of 165899 results

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MSGV Hu Acceptor

Plasmid

#64269

Purpose

Retroviral golden gate cloning vector for expression of functional Human T-Cell Receptor genes

Depositor

David Ott

Article

Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

Type

Empty backbone

Use

Retroviral

Tags

none

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pET3a-OSF-CypA

Plasmid

#79039

Purpose

Bacterial vector for cloning and propagation in DH5alpha

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OneSTrEP-FLAG- Cyclophilin A (PPIA Human)

Tags

OneSTrEP-FLAG

Expression

Bacterial

Promoter

T7

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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DsRed-Rab5-QL

Plasmid

#29688

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

RAB5A

Tags

DSRed2

Expression

Mammalian

Mutation

Q79L

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only
pCS2 DN-GSK3-GFP

Plasmid

#29681

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

glycogen synthase kinase 3

Tags

GFP

Expression

Mammalian

Mutation

K85R and K86R

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
LB901: pMVP/PB/Blast-DEST

Plasmid

#121873

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Blast selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LC201: pMVP/PB/Puro-DEST

Plasmid

#121874

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Puro selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-FRT-TO-GFP-PALB2 KR

Plasmid

#71107

Purpose

mammalian expression of GFP-PALB2 mutant. (first 8 Lysine residues mutated to Arginine)

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

PALB2 (PALB2 Human)

Tags

GFP

Expression

Mammalian

Mutation

first 8 Lysine residues mutated to Arginine (K14-…

Promoter

CMV/TO

Available Since

Dec. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST-mCherry-ARHGEF2

Plasmid

#207959

Purpose

Expression vector for ARHGEF2 (GEF-H1) with an N-terminal mCherry tag

Depositor

Johanna Ivaska

Article

Conway et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

Insert

ARHGEF2 (ARHGEF2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST-HisMBP-SED1

Plasmid

#178191

Purpose

SED1 biosensor for expression in Escherichia coli

Depositor

Cesar Cuevas-Velazquez, Jose Dinneny

Article

Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

Insert

SED1 biosensor

Tags

6xHis-MBP

Expression

Bacterial

Promoter

tac

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pDestTol2-ubi-ASAP1

Plasmid

#121987

Purpose

ubiqutously express ASAP1 voltage biosensor

Depositor

GuangJun Zhang

Article

Silic et al J Vis Exp. 2018 Apr 25;(134). doi: 10.37

Insert

ASAP1

Use

Tol2 transposon destination vector

Promoter

zebrafish ubiquitin promoter

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-deSpCas9

Plasmid

#92114

Purpose

Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

dead/inactive eSpCas9 with FLAG tag

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

D10A, H840A, K848A, K1003A, R1060A

Promoter

Cbh

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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372 pTol2-fli1ep:EGFPDest

Plasmid

#73491

Purpose

Tol2 vector with fli1ep element upstream of EGFP, attR1/R2 cassette, and late SV40 pA signal

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

fli1ep:EGFP-attR1-attR2

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST51-LRRK2-Y1699C

Plasmid

#25084

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Gateway Cloning

Tags

6XHis and V5

Expression

Mammalian

Mutation

Tyrosine 1699 mutated to Cysteine

Available Since

July 2, 2010

Availability

Academic Institutions and Nonprofits only
ER-EGFP-eMagA

Plasmid

#162245

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

CytP450 ER targeting sequencel-EGFP-eMagA

Expression

Mammalian

Promoter

beta-actin promoter

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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Str-STIM1-NN_ST-SBP-EGFP

Plasmid

#65262

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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Ii-Str_SBP-EGFP-GPI

Plasmid

#65296

Purpose

synchronize trafficking of EGFP-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2 GFP-GSK3mut-MAPK

Plasmid

#29690

Purpose

GFP reporter protein fused to three mutated GSK3 phosphorylation sites

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

GFP glycogen synthase kinase 3 recognition site mutated primed by MAPK

Tags

Flag and Flag-EGFP

Expression

Mammalian

Mutation

recognition sites only, and E3 ligase site, GSK3 …

Promoter

SP6

Available Since

Jan. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pENTR-GFI1B

Plasmid

#16169

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Growth factor independent 1B (GFI1B Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
GFP-Paxillin WT

Plasmid

#186152

Purpose

For expressing paxillin variants in mammalian cells

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PXN (PXN Human)

Tags

mEGFP

Expression

Mammalian

Promoter

cmv

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCMW35 p21 (mouse)

Plasmid

#16478

Depositor

Bert Vogelstein

Article

el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

Insert

p21 (Cdkn1a Mouse)

Expression

Bacterial

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only