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C413-E33: attB4-UbCp>-attB1r

Plasmid

#162925

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the human Ubiquitin C promoter

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human Ubiquitin C promoter

Use

Synthetic Biology

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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C413-E31: attB4-TRE3Gp>-attB1r

Plasmid

#162924

Purpose

Gateway attB4/attB1r entry clone for inducible mammalian expression using the TRE3G promoter

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Tet-regulated TRE3G minimal promoter

Use

Synthetic Biology

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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C234-E08: attB2r-3xFLAG-attB3

Plasmid

#162912

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal triple-FLAG epitope tagged fusion proteins

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

triple FLAG epitope tag

Use

Synthetic Biology

Available since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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C232-E05: attB2r-meGFP-attB3

Plasmid

#162902

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal eGFP fusion proteins

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric enhanced Green fluorescent protein

Use

Synthetic Biology

Available since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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C232-E06: attB2r-mCherry-attB3

Plasmid

#162903

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal mCherry fusion proteins

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Cherry fluorescent protein

Use

Synthetic Biology

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E23: attB4-TRE3Gp>-attB5

Plasmid

#162979

Purpose

Gateway attB4/attB5 entry clone for inducible mammalian expression using the TRE3G promoter

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Tet-regulated TRE3G minimal promoter

Use

Synthetic Biology

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E24: attB4-UbCp>-attB5

Plasmid

#162980

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using the human Ubiquitin C promoter

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human Ubiquitin C promoter

Use

Synthetic Biology

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E67: attB5r-RU5u-Strep2x2-nostop-attB1r

Plasmid

#162967

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal dual Strep-tagged fusion proteins

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Dual Strep2 purification tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E44: attB5r-SNAP-nostop-attB1r

Plasmid

#162962

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal SNAP-tagged fusion proteins

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

SNAP tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C232-E29: attB2r-mClover3-attB3

Plasmid

#162906

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal mClover3 fusion proteins

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Clover3 fluorescent protein

Use

Synthetic Biology

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C232-E30: attB2r-mRuby3-attB3

Plasmid

#162907

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal mRuby3 fusion proteins

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Ruby3 fluorescent protein

Use

Synthetic Biology

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E23: attB5r-RU5u-3xFLAG-nostop-attB1r

Plasmid

#162958

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal 3xFLAG-tagged fusion proteins

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

triple FLAG epitope tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E23: attB5r-mRuby3-nostop-attB1r

Plasmid

#162945

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mRuby3 fusion proteins

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Ruby3 fluorescent protein

Use

Synthetic Biology

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E22: attB5r-mClover3-nostop-attB1r

Plasmid

#162944

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mClover3 fusion proteins

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Clover3 fluorescent protein

Use

Synthetic Biology

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E22: attB4-polyhedrinp>-attB1r

Plasmid

#162922

Purpose

Gateway attB4/attB1r entry clone for baculovirus expression using the AcMNPV polyhedrin promoter

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

AcMNPV polyhedrin promoter

Use

Synthetic Biology

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C234-E06: attB2r-FLAG-attB3

Plasmid

#162910

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal FLAG epitope tagged fusion proteins

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

FLAG epitope tag

Use

Synthetic Biology

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C234-E05: attB2r-V5-attB3

Plasmid

#162909

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal V5 epitope tagged fusion proteins

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

V5 epitope tag

Use

Synthetic Biology

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pFUSC9

Plasmid

#50429

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 9.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pDIRECT_10E

Plasmid

#91209

Purpose

Direct Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers , Plant Selection: none

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers

Use

CRISPR

Expression

Plant

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-epegRNA-SV40-puro

Plasmid

#240539

Purpose

Lentiviral backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.

Depositor

Wensheng Wei

Publication

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

epegRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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