We narrowed to 10,125 results for: Uty

1. SIN40C.SFFV.VP64-mArid3a.IRES.GFP

   Plasmid

   #169313

   Purpose

   Constitutive overexpression of murine VP64-Arid3a fusion protein with GFP as reporter fluorescent protein

   Depositor

   Jan-Henning Klusmann

   Article

   Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

   Insert

   VP64-Arid3a (Arid3a Mouse)
   

   Use

   Lentiviral

   Promoter

   SFFV

   Available Since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Rescue myc-PCDHGC5 mRNA

   Plasmid

   #122229

   Purpose

   Rescue mRNA for Protocadherin gamma C5 knock down with sh1 shRNA (plasmid 122227). With five silent mutations at sh1 shRNA target site.

   Depositor

   Angel de Blas

   Article

   Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.

   Insert

   PCDHGC5 (Pcdhgc5 Rat)
   

   Tags

   9E10 cMyc epitope (EQKLISEEDL) was inserted betwe…

   Expression

   Mammalian

   Mutation

   Silent mutations are in five consecutive codons …

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. ORAI1-YFP V107M-L273D-L276D

   Plasmid

   #114183

   Purpose

   ORAI1 channel with C-terminal YFP, carrying the constitutively activating mutation V107M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.

   Depositor

   Nicolas Demaurex

   Article

   Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27

   Insert

   ORAI1-YFP V107M-L273D-L276D (ORAI1 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   V107M/L273D/L276D

   Available Since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-VRER-P2A-GFP-PGK-Puro

   Plasmid

   #110862

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VRER-P2A-GFP (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VRER
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Mutation

   D1135V, G1218R, R1335E, T1337R

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-HF1-P2A-GFP-PGK-Puro

   Plasmid

   #110863

   Purpose

   Lentiviral vector for constitutive expression of Cas9-HF1-P2A-GFP (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-HF1
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJC20cdc8.A18T

   Plasmid

   #99360

   Purpose

   Bacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Ala-18-Thr.

   Depositor

   Dan Mulvihill

   Article

   Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

   Insert

   cdc8 (cdc8 Fission Yeast)
   

   Expression

   Bacterial

   Mutation

   Alanine 18 to Threonine

   Promoter

   T7

   Available Since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJC20cdc8.E31K

   Plasmid

   #99361

   Purpose

   Bacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Glu-31-Lys.

   Depositor

   Dan Mulvihill

   Article

   Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

   Insert

   cdc8 (cdc8 Fission Yeast)
   

   Expression

   Bacterial

   Mutation

   Glutamic acid 31 to Lysine

   Promoter

   T7

   Available Since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti CMV Hygro dn-cHSF1

   Plasmid

   #134732

   Purpose

   Lentiviral expression vector for constitutively active dominant-negative HSF1

   Depositor

   Matthew D. Shoulders

   Article

   Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

   Insert

   dn-cHSF1 (HSF1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 186-202, 379−529

   Promoter

   CMV

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti CMV/TO Zeo dn-cHSF1

   Plasmid

   #134734

   Purpose

   Lentiviral expression vector for inducible expression of a constitutively active dominant-negative HSF1

   Depositor

   Matthew D. Shoulders

   Article

   Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

   Insert

   dn-cHSF1 (HSF1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 186-201, 380−529

   Promoter

   CMV/TO

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDest40 DHFR.dn-cHSF1

    Plasmid

    #134735

    Purpose

    Mammalian expression vector for constitutively active dominant-negative HSF1 fused to DHFR destabilized domain

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

    Insert

    DHFR.dn-cHSF1 (HSF1 Human)
    

    Tags

    DHFR

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 186-202, 379−529

    Promoter

    CMV

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti PGK Puro GFP-GABARAPL1 G116

    Plasmid

    #123243

    Purpose

    Expression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116. For lentivirus production and stable transduction in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deleted amino acid 117. Stop codon after G116

    Promoter

    PGK

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR

    Plasmid

    #68345

    Purpose

    A Sleeping beauty transposon with conditional expressed hCas9. A red flourescent gene linked to puromycin can be removed in the prescence of Flp recombinase allowing Cas9 expression

    Depositor

    Jannik Elverløv-Jakobsen

    Article

    Berthelsen et al Cancers (Basel). 2021 Jun 16;13(12). pii

    Inserts

    puromycin
    hCas9
    

    Use

    CRISPR; Flp/frt

    Tags

    linked to red flouresent protein gene mKateII and…

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLP-PIGA3 (pLP305)

    Plasmid

    #196992

    Purpose

    Big-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites, sleeping beauty ITRs and BsmBI homology arms cloning sites.

    Depositor

    Matthew Maurano

    Article

    Brosh et al Mol Cell. 2023 Apr 6;83(7):1140-1152.e7.

    Type

    Empty backbone

    Use

    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti PGK Puro GFP-GABARAPL1 G116A

    Plasmid

    #123245

    Purpose

    Expression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116A. For lentivirus production and stable transduction in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Glycine 116 to Alanine

    Promoter

    PGK

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMIR-Report-SPRED1-3'UTR (mut - miR-21 site1)

    Plasmid

    #62580

    Purpose

    Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The upstream miR-21 (site 1) binding site was mutated.

    Depositor

    Michael Ruppert

    Article

    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

    Insert

    SPRED1 (SPRED1 Human)
    

    Use

    Luciferase

    Mutation

    miR-21 binding site 1 mutant (TAAGCTA --> TAGA…

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pMIR-Report-SPRED1-3'UTR (mut - miR-21 site 2)

    Plasmid

    #62581

    Purpose

    Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The downstream miR-21 binding site (site 2) was mutated.

    Depositor

    Michael Ruppert

    Article

    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

    Insert

    SPRED1 (SPRED1 Human)
    

    Use

    Luciferase

    Mutation

    miR-21 binding site 2 mutant (TAAGCTA --> TAGA…

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

17. MG1655 Z1 malE

    Bacterial Strain

    #65915

    Purpose

    E. coli MG1655 Z1 host that produces constitutive levels of TetR, LacI and AraC off of the chromosome with additional malE KO

    Depositor

    Keith Tyo

    Article

    Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

    Bacterial Resistance

    None

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pBFC0984

    Plasmid

    #231992

    Purpose

    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with 2xBsaI spacer cloning site

    Depositor

    Brady Cress

    Article

    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d

    Inserts

    dRfxCas13d
    crRNA with 2xBsaI spacer
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119 and pTet

    Available Since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLIK MEK5-DD-mRFP1 neo

    Plasmid

    #47548

    Purpose

    Lentiviral expression vector for an inducible mRFP1-tagged constitutively active MEK5 mutant

    Depositor

    Kevin Janes

    Article

    Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

    Insert

    MEK5-DD-mRFP1 (MAP2K5 Human)
    

    Use

    Lentiviral

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    S311D, T315D

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

20. 3` CC: Hygro – H2B-GFP-N – loxP – mScarlet

    Plasmid

    #219565

    Purpose

    3` circularization cassette to induce Cre-mediated circularization of a genomic region flanked by loxP sites with H2B-GFP reconstitution and mScarlet expression from the chromosome

    Depositor

    Andrea Ventura

    Article

    Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

    Insert

    HygroR_2A_H2B-GFP-N_loxP_mScarlet
    

    Use

    Unspecified

    Promoter

    EF-1a

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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