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pEF1a-EGFP-hs-miR-126 (JDW 1415)

Plasmid

#242581

Purpose

An EF1a driven expression vector containing an EGFP fused to the human microRNA 126.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EGFP with an intron containing hs-miR-126 (MIR126 Human)

Expression

Mammalian

Promoter

human EF1a

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pBC001 v3

Plasmid

#161711

Purpose

CMVp-EGFP-[barcode cloning site]-PGKp-Puro-WPRE

Depositor

Nozomu Yachie

Article

Nakayama et al Sci Immunol. 2024 May 24;9(95):eade3814.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

CMV Promoter

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pL(CMVtight-phi-AQP1-FKBP12DD-IRES-EGFP-WPRE)

Plasmid

#236278

Purpose

Lentiviral vector that can express doxycycline-inducible hAqp1 with fkbp12 degron in Tet ON mammalian cells. CMVtight promoter. EGFP marker.

Depositor

Arnab Mukherjee

Article

Yun et al Chem Sci. 2024 Jun 4;15(28):11108-11121.

Insert

human aquaporin 1 (AQP1 Human)

Use

Lentiviral

Tags

Flag and fkbp12

Expression

Mammalian

Promoter

CMVtight

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pGH020 sgZmat3.1

Plasmid

#227943

Purpose

sgRNA targeting Zmat3, GFP, neomycin resistance, Mammalian expression, Mouse targeting, lentiviral, CRISPR

Depositor

Laura Attardi

Article

Boutelle et al Cell Death Differ. 2025 Apr 22. doi: 10.

Insert

Zmat3.1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

human U6

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pGH020 sgZmat3.2

Plasmid

#227944

Purpose

sgRNA targeting Zmat3, GFP, neomycin resistance, Mammalian expression, Mouse targeting, lentiviral, CRISPR

Depositor

Laura Attardi

Article

Boutelle et al Cell Death Differ. 2025 Apr 22. doi: 10.

Insert

Zmat3.2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

human U6

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSOX17-T2A-dTom-PGK-Neo

Plasmid

#223190

Purpose

Tomato reporter plasmid targeting the human SOX17 gene

Depositor

Matthew Birket

Article

Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

Insert

SOX17-T2A-dTomato-PGK-Neo-SOX17 (SOX17 Human)

Use

Cre/Lox; Human targeting

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-FLAG-HAND1-T2A-mTagBFP2

Plasmid

#223192

Purpose

Entry vector containing FLAG-HAND1 with a T2A-mTagBFP2 reporter (attL flanked)

Depositor

Matthew Birket

Article

Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

Insert

HAND1 (HAND1 Human)

Use

Promoterless entry vector for gateway cloning

Tags

FLAG and T2A-mTagBFP2

Expression

Bacterial

Promoter

None

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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p5E-Dll4-F2-E1B-B-Globin (JDW 1366)

Plasmid

#229843

Purpose

A gateway compatible 5' entry clone containing the murine Dll4 F2 arterial specific enhancer upstream of a minimal E1b promoter and intron for arterial and endocardial expression.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Dll4-F2 (exon3)

Use

Gateway entry clone

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-Ubi-lexPR-nls-GFP-pA (JDW 1316)

Plasmid

#229811

Purpose

A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus (1st generation)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA transactivator-LexA-Operon-nls-GFP

Use

Tol2 based expression vector

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-Ubi-lexA-oMDC-pA-lexO-cFos-nlsGFP-pA (JDW 1382)

Plasmid

#229813

Purpose

A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator and a c-fos minimal promoter.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA-mODC-LexAOp-cFos

Use

Tol2 based expression vector

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA-2xIns-fli1ep-zCreI-BFP (JDW 1218)

Plasmid

#229819

Purpose

A gateway compatible Tol2 destination vector containing the fli1a promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

Tol2 destination vector

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Tet-Off-FLEX-DEST (JDW 1186)

Plasmid

#229820

Purpose

An AAV, tet-off, gateway-compatible destination vector with cre-dependent expression of the insert. EFS driving destablized rTA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

AAV

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-Luciferase-P2A-H2A-mCherry (JDW 1129)

Plasmid

#229823

Purpose

A CAGGS driven luciferase reporter followed by a P2A cleavage peptide and an H2A mCherry cassette for nuclear labeling.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Luciferase-P2A-H2A-mCherry

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-DEST-hsp70-zCreI-BFP (JDW 1184)

Plasmid

#229831

Purpose

A gateway compatible Tol2 destination vector containing the hsp70 promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

Tol2 destination vector

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-Ubi-LexA-oMDC-pA-LexOp-nlsGFP-pA (JDW 1381)

Plasmid

#229838

Purpose

A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA-mODC-LexAOp

Use

Tol2 based expression vector

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTag4C_SP-DRD2-V2R-NTEV-tcs-GV-2xHA

Plasmid

#227103

Purpose

DRD2 receptor for split TEV assay

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

DRD2-NTEV (DRD2 Human)

Tags

2xHA

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-EFS (EF1a core) (JDW 1319)

Plasmid

#224474

Purpose

Gateway compatible 5' entry clone with human alpha 2 globin pause site (to prevent transcription readthrough) and EF1a core promoter. Lacks downstream intron for a lower-activity, compact promoter.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EFS promoter

Expression

Mammalian

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pME-LexA-mODC-LexOP-35SCaMV (JDW 1348)

Plasmid

#224505

Purpose

Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with minimal CaMV 35S promoter as an all in one casette for RU486 driven gene expression

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA-mODC-LexOP-35SCaMV

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-LexA-mODC-LexOP-c-Fos (JDW 1359)

Plasmid

#224506

Purpose

Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with 3' minimal c-Fos promoter for an all in one RU486 driven gene expression construct

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA-oMDC-LexOP-c-Fos

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-3xHA-BirA-T2A-mCherry-caax (JDW 889)

Plasmid

#224512

Purpose

A Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

3xHA-BirA-T2A-mCherry-Caax

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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