We narrowed to 11,580 results for: Vars;

1. U6-(BbsI)sgRNA_CAG-Venus-bpA

   Plasmid

   #86985

   Purpose

   For cloning and expression of sgRNA together with Venus expression

   Depositor

   Ralf Kuehn

   Article

   Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

   Insert

   Venus
   

   Expression

   Mammalian

   Mutation

   Venus GFP variant

   Promoter

   CAG

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBac{3XP3::DsRed, Pactin::Ptrsps}

   Plasmid

   #86862

   Purpose

   Expresses DsRed in eyes and ubiquitous expression of P-transposase

   Depositor

   David Stern

   Article

   Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

   Insert

   pACNNTNPII-S129A
   

   Expression

   Insect

   Mutation

   S129A

   Available Since

   June 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. scFv-IFP2.0N

   Plasmid

   #215746

   Purpose

   Expresses single chain Fragment variable (scFv) fused to N-terminal part of split IFP2.0

   Depositor

   Albert Jeltsch

   Article

   Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

   Insert

   IFP2.0N
   

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDL17_Cas9-His Δcys M1C

   Plasmid

   #206251

   Purpose

   Bacterial expression of SpCas9 Δcys M1C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   M1C, C80S, C574S

   Promoter

   T7

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTEV5_ RpMatB_1

   Plasmid

   #78205

   Purpose

   Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor

   Depositor

   Martin Webb

   Article

   Vancraenenbroeck et al ACS Chem Biol. 2015 Nov 20;10(11):2650-7

   Insert

   matB (His6 C106A R286C Q457C K488A)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   C106A R286C Q457C K488A

   Promoter

   T7

   Available Since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

6. P305-FBP_4

   Plasmid

   #139695

   Purpose

   T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

   Depositor

   Daniel Voytas

   Article

   Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

   Insert

   pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:tAtuOCS, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
   

   Use

   Luciferase

   Expression

   Plant

   Promoter

   various

   Available Since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDS220

   Plasmid

   #34980

   Purpose

   Expresses a low-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   ePDZb–mCherry
   

   Expression

   Yeast

   Promoter

   TEF

   Available Since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-AAAAA-nCas9(D10A)-UGI

   Plasmid

   #187409

   Purpose

   DddA AAAAA variant

   Depositor

   Jin-Soo Kim

   Article

   Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

   Insert

   DddA AAAAA
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLP-pR-himar-apraR

   Plasmid

   #199996

   Purpose

   Landing Pad with T7 RNAP circuit variant T15. pC194+Tn903 seed promoter. pR-himar transposase. In dap auxotrophic conjugation donor

   Depositor

   Farren Isaacs

   Article

   Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

   Insert

   Landing Pads
   

   Expression

   Bacterial

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. 1/8-NORAD-(A)4x

    Plasmid

    #199209

    Purpose

    1/8 truncation of NORAD mRNA tagged with 4 copies of Riboglow (variant A)

    Depositor

    Esther Braselmann

    Article

    Sarfraz et al Nat Commun. 2023 Feb 16;14(1):867. doi:

    Insert

    1/8-NORAD
    

    Tags

    Riboglow RNA tag

    Expression

    Mammalian

    Mutation

    4 copies of Riboglow tag after stop codon

    Promoter

    CMV

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. 1/2-NORAD-(A)4x

    Plasmid

    #199208

    Purpose

    1/2 truncation of NORAD mRNA tagged with 4 copies of Riboglow (variant A)

    Depositor

    Esther Braselmann

    Article

    Sarfraz et al Nat Commun. 2023 Feb 16;14(1):867. doi:

    Insert

    1/2-NORAD
    

    Tags

    Riboglow RNA tag

    Expression

    Mammalian

    Mutation

    4 copies of Riboglow tag after stop codon

    Promoter

    CMV

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBac{3XP3::EGFP, Pactin::Ptrsps}

    Plasmid

    #86861

    Purpose

    Expresses EGFP in eyes and ubiquitous expression of P-transposase

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

    Insert

    pACNNTNPII-S129A
    

    Expression

    Insect

    Available Since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. UAS:Zebrabow-V

    Plasmid

    #118218

    Purpose

    For broad or variegated gal4 inducible expression of Brainbow in zebrafish

    Depositor

    Alex Schier, Albert Pan

    Article

    Pan et al Development. 2013 Jul;140(13):2835-46. d

    Insert

    Brainbow 1.0 L
    

    Use

    Zebrafish expression

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. px458 EQR

    Plasmid

    #101731

    Purpose

    Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 EQR
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1_hMed25

    Plasmid

    #206958

    Purpose

    The ORF of human Med25 cDNA amplified from the RT human liver RNA was cloned into pGEMTE vector and then subclone at XhoI/XbaI sites of the pcDNA3.2(+), the mammalian expression vector.

    Depositor

    Reza Zolfaghari, Catharine Ross

    Insert

    Homo sapiens mediator complex subunit 25 (MED25), transcript variant 1, mRNA (MED25 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pARR5::LUC

    Plasmid

    #141170

    Purpose

    Luminescent reporter for cytokinin signaling

    Depositor

    Patrick Schäfer, Vardis Ntoukakis

    Article

    Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

    Insert

    AtARR5 promoter
    

    Use

    Luciferase

    Expression

    Plant

    Promoter

    AtARR5, AT3G48100

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGP-RSET-iGABASnFR2n-6xHis

    Plasmid

    #218886

    Purpose

    Bacterial expression of improved GABA sensor (negative change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2n
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S99A F104H R168P

    Promoter

    T7

    Available Since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. CAG:: ChR2HA-2a-hM4D

    Plasmid

    #52520

    Purpose

    Co-expresses HA tagged channelrhodopsin and hM4D (Gi)

    Depositor

    Scott Sternson

    Article

    Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

    Insert

    ChR2-2a-hM4D
    

    Tags

    2xHA on ChR2

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRAB18::LUC

    Plasmid

    #141168

    Purpose

    Luminescent reporter for abscisic acid signaling

    Depositor

    Patrick Schäfer, Vardis Ntoukakis

    Article

    Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

    Insert

    AtRAB18 promoter
    

    Use

    Luciferase

    Expression

    Plant

    Promoter

    AtRAB18, AT5G66400

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET30 GLTP

    Plasmid

    #170733

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Malinina et al Nature. 2004 Aug 26;430(7003):1048-53. d

    Insert

    GLTP (GLTP Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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