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NFATc2-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75232

Purpose

CRISPR/Cas9 plasmid against human NFATc2

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV_EE-NG-nCas9

Plasmid

#163542

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

EE-NG-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4xEcoLeuT(CUA)_EF1_AnapRS

Plasmid

#174894

Purpose

AnapRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

AnapRS

Expression

Mammalian

Mutation

L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_EIF5A2

Plasmid

#45266

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

EIF5A2 (EIF5A2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pBAD mApple

Plasmid

#91772

Purpose

Bacterial expression of a fluorescent protein, monomeric Apple (mApple). Note: Addgene identified several mutations compared to the final published sequence.

Depositor

Erik Rodriguez, Roger Tsien

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

monomeric Apple

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Mutation

R97K, E152S, A180S, V207T compared to mApple (FP …

Promoter

araBAD

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pTCO2-mTDGi.0

Plasmid

#149429

Purpose

miniTdg gene

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Insert

Tdg (Tdg Mouse)

Use

Cre/Lox

Expression

Mammalian

Mutation

SNPs of the Tdg gene from the OLA/129 strain

Promoter

Tdg promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp5

Plasmid

#157685

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp5 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLX304_zeo_eGFP_V5

Plasmid

#160096

Purpose

Expresses eGFP in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

eGFP

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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shYES1 # 2

Plasmid

#42547

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YES1 (YES1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT/TO-neo-C11orf51-IRES2-mRuby (2763)

Plasmid

#39858

Depositor

Jonathon Pines

Article

Mansfeld et al Nat Cell Biol. 2011 Sep 18;13(10):1234-4

Insert

C11orf51 (ANAPC15 Human)

Tags

IRES2-mRuby

Expression

Mammalian

Available Since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only
pMB37

Plasmid

#73084

Purpose

Expresses C. elegans optimized BirA under control of the ubiquitous rps-27 promoter

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

BirA

Expression

Worm

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pQStrep2-GADD45G

Plasmid

#31584

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

growth arrest and DNA-damage-inducible, gamma (GADD45G Human)

Tags

His and Strep

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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shYES1 # 1

Plasmid

#42546

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YES1 (YES1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
2xNLS-NLP-cMyc LbaCas12a

Plasmid

#182123

Purpose

pET21a protein expression vector for 2xNLS-NLP-cMyc LbaCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

2xNLS-NLP-cMyc LbaCas12a

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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A*01:03:01

Plasmid

#203293

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*01:03:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pRF+275Dux4

Plasmid

#21293

Depositor

Stephen Tapscott

Article

Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

Insert

DUX 4 (DUX4 DUX 4, Human)

Use

Luciferase

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT-TO-FH-Nsp1

Plasmid

#157680

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp1 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp1 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pX330A-Yap1-E1-#1

Plasmid

#171517

Purpose

deletion of a genomic locus in Yap1 gene

Depositor

Hans Schöler

Article

Kagiwada et al Development. 2021 Oct 15;148(20). pii: 2

Insert

Yap1 (Yap1 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pX330A-Yap1-E2-#1

Plasmid

#171518

Purpose

deletion of a genomic locus in Yap1 gene

Depositor

Hans Schöler

Article

Kagiwada et al Development. 2021 Oct 15;148(20). pii: 2

Insert

Yap1 (Yap1 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMB73

Plasmid

#73086

Purpose

Expresses C. elegans optimized BirA under control of the seam and hypodermis wrt-2 promoter

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

BirA

Expression

Worm

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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