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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,380 results for: Cre

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Showing: 9761 - 9780 of 14380 results

  1. pX330-Flag-Sniper SpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126777
    Purpose
    Expression plasmid for human codon-optimized increased fidelity Sniper SpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sniper SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    F539S, M763I, K890N
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126777-plasmid-map-sequence-id-410581

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  2. B-HeFSpCas9

    Plasmid
    #126766
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-HeFSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HeFSpCas9.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    B-HeFSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; R661A; Q695A; K848A; Q926A; K1003A; R1060A…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126766-plasmid-map-sequence-id-259607

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  3. GS2.1/EC

    Plasmid
    #132568
    Purpose
    sgRNA targeting A. thaliana pds3, regulated by A. thaliana U6 polIII promoter. Cas9 with a C-terminal mApple fusion, egg cell-specific expression. Hygromycin selectable marker (hpt).
    Depositor
    Mathias Pribil
    Publication
    Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111
    Insert
    egg cell promoter, Cas9-mApple, pds3 sgRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Promoter
    A. thaliana egg cell promoter
    Available since
    Dec. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    132568-plasmid-map-sequence-id-262634

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  4. Mouse 5' Srcap AID GFP Puro

    Plasmid
    #127903
    Purpose
    HDR (donor) Plasmid for Inserting PuroR-2A-GFP-AID into the 5' end of the Mouse Srcap Gene
    Depositor
    Mark Groudine
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Srcap (Srcap Mustard Weed, Mouse)
    Use
    Donor plasmid
    Tags
    GFP, AID, PuroR
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127903-plasmid-map-sequence-id-251069

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  7. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide3

    Plasmid
    #118164
    Purpose
    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA3 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Publication
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hPGK and U6
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118164-plasmid-map-sequence-id-240433

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  8. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR GLIS3 TSS-guide3

    Plasmid
    #118175
    Purpose
    CRISPR-mediated repression of GLIS3. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.
    Depositor
    Jorge Ferrer
    Publication
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hPGK and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118175-plasmid-map-sequence-id-240390

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  9. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide3

    Plasmid
    #118171
    Purpose
    CRISPR-mediated repression of TCF7L2. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.
    Depositor
    Jorge Ferrer
    Publication
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hPGK and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118171-plasmid-map-sequence-id-240389

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  10. BMR1_03g00790-bio-His

    Plasmid
    #108023
    Purpose
    Expresses enzymatically monobiotinylated full-length BMR1_03g00790 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Elton et al Int J Parasitol. 2019 Feb;49(2):115-125.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BMR1_03g00790
    Tags
    ratCD4d3+4,biotinylation sequence, 6xHis
    Expression
    Mammalian
    Mutation
    all potential N-linked glycosylation sites (N-X-S…
    Promoter
    CMV
    Available since
    Oct. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    108023-plasmid-map-sequence-id-213187

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  11. BMR1_03g02515-bio-His

    Plasmid
    #108036
    Purpose
    Expresses enzymatically monobiotinylated full-length BMR1_03g02515 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Elton et al Int J Parasitol. 2019 Feb;49(2):115-125.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BMR1_03g02515
    Tags
    ratCD4d3+4,biotinylation sequence, 6xHis
    Expression
    Mammalian
    Mutation
    all potential N-linked glycosylation sites (N-X-S…
    Promoter
    CMV
    Available since
    Oct. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    108036-plasmid-map-sequence-id-213066

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  12. BMR1_03g02385-bio-His

    Plasmid
    #108040
    Purpose
    Expresses enzymatically monobiotinylated full-length BMR1_03g02385 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Elton et al Int J Parasitol. 2019 Feb;49(2):115-125.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BMR1_03g02385
    Tags
    ratCD4d3+4,biotinylation sequence, 6xHis
    Expression
    Mammalian
    Mutation
    all potential N-linked glycosylation sites (N-X-S…
    Promoter
    CMV
    Available since
    Oct. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    108040-plasmid-map-sequence-id-213057

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  13. BMR1_01g01510-bio-His

    Plasmid
    #108019
    Purpose
    Expresses enzymatically monobiotinylated full-length BMR1_01g01510 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Elton et al Int J Parasitol. 2019 Feb;49(2):115-125.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BMR1_01g01510
    Tags
    ratCD4d3+4,biotinylation sequence, 6xHis
    Expression
    Mammalian
    Mutation
    all potential N-linked glycosylation sites (N-X-S…
    Promoter
    CMV
    Available since
    Oct. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    108019-plasmid-map-sequence-id-210761

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  14. BMR1_01g02875-bio-His

    Plasmid
    #108030
    Purpose
    Expresses enzymatically monobiotinylated full-length BMR1_01g02875 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Elton et al Int J Parasitol. 2019 Feb;49(2):115-125.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BMR1_01g02875
    Tags
    ratCD4d3+4,biotinylation sequence, 6xHis
    Expression
    Mammalian
    Mutation
    all potential N-linked glycosylation sites (N-X-S…
    Promoter
    CMV
    Available since
    Oct. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    108030-plasmid-map-sequence-id-210763

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  15. BMR1_03g02090-bio-His

    Plasmid
    #107674
    Purpose
    Expresses enzymatically monobiotinylated full-length BMR1_03g02090 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Elton et al Int J Parasitol. 2019 Feb;49(2):115-125.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BMR1_03g02090
    Tags
    ratCD4d3+4,biotinylation sequence, 6xHis
    Expression
    Mammalian
    Mutation
    all potential N-linked glycosylation sites (N-X-S…
    Promoter
    CMV
    Available since
    Oct. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    107674-plasmid-map-sequence-id-210740

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  16. BMR1_01g00985-bio-His

    Plasmid
    #107675
    Purpose
    Expresses enzymatically monobiotinylated full-length BMR1_01g00985 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Elton et al Int J Parasitol. 2019 Feb;49(2):115-125.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BMR1_01g00985
    Tags
    ratCD4d3+4,biotinylation sequence, 6xHis
    Expression
    Mammalian
    Mutation
    all potential N-linked glycosylation sites (N-X-S…
    Promoter
    CMV
    Available since
    Oct. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    107675-plasmid-map-sequence-id-213195

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  17. BMR1_03g04335-bio-His

    Plasmid
    #108000
    Purpose
    Expresses enzymatically monobiotinylated full-length BMR1_03g04335 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Elton et al Int J Parasitol. 2019 Feb;49(2):115-125.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BMR1_03g04335
    Tags
    ratCD4d3+4,biotinylation sequence, 6xHis
    Expression
    Mammalian
    Mutation
    all potential N-linked glycosylation sites (N-X-S…
    Promoter
    CMV
    Available since
    Oct. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    108000-plasmid-map-sequence-id-213068

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  18. BMR1_04g09190-bio-His

    Plasmid
    #108007
    Purpose
    Expresses enzymatically monobiotinylated full-length BMR1_04g09190 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Elton et al Int J Parasitol. 2019 Feb;49(2):115-125.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BMR1_04g09190
    Tags
    ratCD4d3+4,biotinylation sequence, 6xHis
    Expression
    Mammalian
    Mutation
    all potential N-linked glycosylation sites (N-X-S…
    Promoter
    CMV
    Available since
    Oct. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    108007-plasmid-map-sequence-id-211186

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Showing: 9761 - 9780 of 14380 results