Addgene

We narrowed to 45,803 results for: CAT

Showing: 961 - 980 of 45803 results

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pRK5 FLAG 17xS/T->A, catalytic dead lipin 1

Plasmid

#32008

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

Lipin1 (Lpin1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

D712E, D714E AND S106A, S150A, (S281A, T282A), S2…

Promoter

CMV, SP6

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only
mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax

Plasmid

#173868

Purpose

Mammalian expression of mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax (opto-Rap)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax

Use

Tags

C-terminal CAAX-box and mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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mVenus-NES-SspB-SOS2cat-P2A-iLIDcaax

Plasmid

#173867

Purpose

Mammalian expression of mVenus-NES-SspB-SOS2cat-P2A-iLIDcaax (opto-Ras)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mVenus-NES-SspB-SOS2cat-P2A-iLIDcaax

Use

Tags

C-terminal CAAX-box and mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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XE70 NLS Beta-catenin GFP in CS2+

Plasmid

#16838

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1997 Oct 6. 139(1):229-43.

Insert

NLS beta-catenin GFP (ctnnb1.L Frog)

Use

Xenopus expression

Tags

GFP

Expression

Mutation

Promoter

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
KaiC CI cat-/EE (E77Q; E78Q; S431E; T432E)

Plasmid

#41888

Depositor

Michael Rust

Article

Phong et al Proc Natl Acad Sci U S A. 2013 Jan 15;11

Insert

Kai C CI cat-/EE

Use

Tags

Expression

Bacterial

Mutation

changed amin acids: 77,78,431 and 432 to glutamate

Promoter

t7

Available since

March 19, 2013

Availability

Academic Institutions and Nonprofits only
XE71 NES Beta-catenin GFP in CS2+

Plasmid

#16837

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1997 Oct 6. 139(1):229-43.

Insert

NES beta-catenin GFP (ctnnb1.L Frog)

Use

Xenopus expression

Tags

GFP

Expression

Mutation

Promoter

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pBMN-flag beta-catenin K19R/K49R

Plasmid

#44751

Depositor

Eric Fearon

Article

Winer et al J Biol Chem. 2006 Sep 8;281(36):26181-7.

Insert

beta-catenin

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

K19R/K49R

Promoter

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
pET23a-His-TEV-HaloTag-linker-SpyCatcher3

Plasmid

#235150

Purpose

Bacterial expression of His-tagged HaloTag-linker-SpyCatcher3 separated by a TEV protease recognition site.

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

HaloTag linked to SpyCatcher3

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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TfR-SpyCatcher003(K31TAG)-sfGFP-MycTag-CTag

Plasmid

#210021

Purpose

Expression of transferrin receptor transmembrane domain and photocaged SpyCatcher003(K31HCK)-sfGFP on mammalian cell surface.

Depositor

Vesa Hytönen

Article

Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

Insert

Transferrin receptor transmembrane domain-SpyCatcher003(K31TAG)-superfolderGFP-MycTag-CTag (TFRC Synthetic, Human)

Use

Tags

MycTag, CTag

Expression

Mammalian

Mutation

Transferrin receptor transmembrane domain with Y2…

Promoter

CMV promoter

Available since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Myr-DnaJB1(H33Q)-PKAcat-mCherry

Plasmid

#181855

Purpose

mCherry-tagged DnaJB1-PKA catalytic subunit fusion N-terminal myristoylation site and H33Q DnaJB1 mutation to block Hsp70 binding; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

Myr-DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Histidine 33 in DnaJB1 changed to glutamine.

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA-SOScat

Plasmid

#178871

Purpose

Mammalian expression of SOS1(Rem-Cdc25) fused to PB1-AzamiGreen-FRB-TEVcs-mTagBFP2-HA

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA-SOS1(Rem-Cdc25) (SOS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA-Vav2cat

Plasmid

#178870

Purpose

Mammalian expression of Vav2(DH-PH-CRD) fused to PB1-AzamiGreen-FRB-TEVcs-mTagBFP2-HA

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA-Vav2(DH-PH-CRD) (Vav2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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KaiC CII cat-/EE (E318Q; S431E; T432E)

Plasmid

#42498

Depositor

Michael Rust

Article

Phong et al Proc Natl Acad Sci U S A. 2013 Jan 15;11

Insert

KaiC CII cat-/EE (E318Q; S431E; T432E)

Use

Tags

Expression

Bacterial

Mutation

E318Q; S431E; T432E

Promoter

t7

Available since

March 4, 2013

Availability

Academic Institutions and Nonprofits only
Human Beta-catenin GFP

Plasmid

#71367

Purpose

mammalian expression of human Beta-catenin GFP

Depositor

Alpha Yap

Article

Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

Insert

Beta-catenin (CTNNB1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-beta-catenin deltaN90

Plasmid

#36985

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

beta-catenin deltaN90 (CTNNB1 Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

Deletion of amino acids 1-90 (Constitutively Acti…

Promoter

EF1a

Available since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only

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EGFP-Talin head-SpyCatcher003

Plasmid

#133566

Purpose

Expresses EGFP-Talin head-SpyCatcher003 in mammalian cells

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

EGFP-Talin head-SpyCatcher003

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCI-neo beta catenin WT

Plasmid

#16518

Depositor

Bert Vogelstein

Article

Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

Insert

beta catenin (CTNNB1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

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human beta-catenin pcDNA3

Plasmid

#16828

Purpose

Mammalian expression of human beta-catenin with Flag tag

Depositor

Eric Fearon

Article

Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

Insert

beta-catenin (CTNNB1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-S33Y Beta-catenin

Plasmid

#19286

Purpose

Mammalian expression of human beta-catenin with Flag tag and S33Y mutation

Depositor

Eric Fearon

Article

Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

Insert

beta-catenin (CTNNB1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S33Y (presumptive GSK3β phosphorylation site)

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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AKT-translocation-mStrawberry reporter

Plasmid

#158685

Purpose

This plasmid is a AKT pathway reporter. It has 1EF1a promoter driving the expression of truncated FoxO1 fused to mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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