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Plasmid#214280PurposeExpress split-Rac1 fragments that are fused with CIDs and FPsDepositorInsertsUseTagsmCerulean (on insert 1) and mVenus (on insert 2)ExpressionMammalianMutationRac1-Q61LPromoterCMVAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only
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pIRES-mCerulean-FRB-Rac1/N12-Rac1/13C(T17N)-FKBP-mVenus-CAAX
Plasmid#214281PurposeExpress split-Rac1 fragments that are fused with CIDs and FPsDepositorInsertsUseTagsmCerulean (N terminal on insert 1) and mVenus (on…ExpressionMammalianMutationRac1-T17NPromoterCMVAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES-mCerulean-FRB-RhoA/N12-RhoA/13C(Q63L)-FKBP-mVenus-CAAX
Plasmid#214282PurposeExpress split-RhoA fragments that are fused with CIDs and FPsDepositorInsertsUseTagsmCerulean (on insert 1) and mVenus (on insert 2)ExpressionMammalianMutationRhoA-Q63LPromoterCMVAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES-mCerulean-FRB-RhoA/N12-RhoA/13C(T19N)-FKBP-mVenus-CAAX
Plasmid#214283PurposeExpress split-RhoA fragments that are fused with CIDs and FPsDepositorInsertsUseTagsmCerulean (N terminal on insert 1) and mVenus (on…ExpressionMammalianMutationRhoA-T19NPromoterCMVAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES-mCerulean-FRB-KRas/N12-KRas/13C(Q61L)-FKBP-mVenus-CAAX
Plasmid#214284PurposeExpress split-KRas fragments that are fused with CIDs and FPsDepositorInsertsUseTagsmCerulean (on insert 1) and mVenus (on insert 2)ExpressionMammalianMutationKRas-Q61LPromoterCMVAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES-mCerulean-FRB-KRas/N12-KRas/13C(S17N)-FKBP-mVenus-CAAX
Plasmid#214285PurposeExpress split-KRas fragments that are fused with CIDs and FPsDepositorInsertsUseTagsmCerulean (N terminal on insert 1) and mVenus (on…ExpressionMammalianMutationKRas-S17NPromoterCMVAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES-mCerulean-GID1-KRas/N12-KRas/13C(Q61L)-GAI1-92-mVenus-CAAX
Plasmid#214286PurposeExpress split-KRas fragments that are fused with CIDs and FPsDepositorInsertsUseTagsmCerulean (N terminal on insert 1) and mVenus (on…ExpressionMammalianMutationKRas-Q61LPromoterCMVAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES-mCerulean-GID1-KRas/N12-KRas/13C(S17N)-GAI1-92-mVenus-CAAX
Plasmid#214287PurposeExpress split-KRas fragments that are fused with CIDs and FPsDepositorInsertsUseTagsmCerulean (on insert 1) and mVenus (on insert 2)ExpressionMammalianMutationKRas-S17NPromoterCMVAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDY32 AAVS1-PuroR-9xTet0-minCMV-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#161928PurposeDonor plasmid for integrating minCMV promoter-driven dual surface marker/fluorescent reporter at AAVS1 locusDepositorInsertSurface marker and Citrine activation reporter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpEFAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJT039 AAVS1-PuroR-9xTet0-pEF-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#161927PurposeDonor plasmid for integrating pEF promoter-driven dual surface marker/fluorescent reporter at AAVS1 locusDepositorInsertSurface marker and Citrine repression reporter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpEFAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCL055 AAVS1-9xTetO-UbC-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#188747PurposeDonor vector for integrating UbC promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locusDepositorInsertSurface marker and mCitrine reporter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterUbCAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL026 AAVS1-puroR-9xTet0-pPGK-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#196545PurposeDonor plasmid for integrating PGK promoter-driven dual surface marker/fluorescent reporter at AAVS1 locusDepositorInsertSurface marker and Citrine reporter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
DENV-3 strain Phillippines/H87/1956 NS2B-NS3 protease fusion with non cleavable linker
Plasmid#228651PurposeBacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 3 for biochemical assaysDepositorInsertDengue 3 portion of NS2B protease fused to NS3
UseTagsHis-Twin Strep-TevExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Enterovirus A71 (EV-A71) isolate 4643-TW98 3C protease with non-cleavable C-terminal His AviTag
Plasmid#228635PurposeBacterial expression of the A71 3C protease for assays that require immobilization using biotin-streptavidin interactionDepositorInsertEnterovirus A71 3C protease isoform A
UseTagsHis-AviExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Enterovirus A71 (EV-A71) isolate 4643-TW98 2A protease with non-cleavable C-terminal His AviTag
Plasmid#228634PurposeBacterial expression of the A71 2A protease for assays that require immobilization using biotin-streptavidin interactionDepositorInsertEnterovirus Coxsackievirus A71 2A protease
UseTagsHis-AviExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCL025 AAVS1-9xTetO-RSV-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#188743PurposeDonor vector for integrating RSV promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locusDepositorInsertSurface marker and mCitrine reporter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterRSVAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL026 AAVS1-9xTetO-hPGK-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#188744PurposeDonor vector for integrating PGK promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locusDepositorInsertSurface marker and mCitrine reporter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterPGKAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL051 AAVS1-9xTetO-nTATAchr21-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#188745PurposeDonor vector for integrating nonTATAchr21 promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locusDepositorInsertSurface marker and mCitrine reporter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoternonTATAchr21Available sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL053 AAVS1-9xTetO-nTATAchrX-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#188746PurposeDonor vector for integrating nonTATAchrX promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locusDepositorInsertSurface marker and mCitrine reporter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoternonTATAchrXAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
EV-A71 2A protease; aliases: Enterovirus EV71 2A protease, 6xHis-SUMO-TEV tag, self cleaving
Plasmid#204810PurposeBacterial expresison of Enterovirus A71 2A proteaseDepositorInsertA71 2A protease
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits