We narrowed to 1,007 results for: Psp

1. INS-3'gRNA

   Plasmid

   #210468

   Purpose

   gRNA targeting INS 3' terminal for CRISPR-Cas9-mediated knock-in

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Insert

   INS (INS Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS10-riboE-dddAI

   Plasmid

   #186561

   Purpose

   Regulated expression of the DddA immunity determinant (DddAI) in Firmicutes

   Depositor

   Joseph Mougous

   Article

   Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

   Insert

   dddAI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pSpac(hy)

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. rd

   Plasmid

   #112912

   Purpose

   Express the PEST domain-replaced 48 kDa form of human dematin with a cleavable N-terminal GST tag

   Depositor

   C. James McKnight

   Article

   Chen et al J Biol Chem. 2013 Mar 22;288(12):8313-20

   Insert

   dematin (DMTN Human)
   

   Use

   Tags

   Glutatione-S-Tranferase and precision protease si…

   Expression

   Bacterial

   Mutation

   PEST sequence near the N-terminal removed. 5′-GCG…

   Promoter

   T7

   Available since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX459-CTNNB1-ATG

   Plasmid

   #153429

   Purpose

   Expresses a gRNA that overlaps the startcodon of human CTNNB1

   Depositor

   Renee van Amerongen

   Article

   de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

   Insert

   CTNNB1 gRNA (CTNNB1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. PX459v3

   Plasmid

   #178799

   Purpose

   SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh (Cas9-2A Puro) and U6 (gRNA)

   Available since

   Aug. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX459-CTNNB1-S45

   Plasmid

   #164587

   Purpose

   Expresses a gRNA that overlaps the S45 codon of human CTNNB1

   Depositor

   Renee van Amerongen

   Article

   de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

   Insert

   CTNNB1 gRNA (CTNNB1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. PX458-sgTSC2

   Plasmid

   #128098

   Purpose

   CRISPR gRNA against human TSC2 with Cas9 from S. pyogenes and 2A-EGFP

   Depositor

   Chi Van Dang

   Article

   Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

   Insert

   sgRNA against human TSC2 (TSC2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. CCND2 targeting gRNA

   Plasmid

   #215318

   Purpose

   Expresses gRNA targeting CCND2 and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. CCND1 targeting gRNA

   Plasmid

   #215153

   Purpose

   Expresses gRNA targeting CCND1 and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CDKN1A (p21) targeting gRNA

    Plasmid

    #215319

    Purpose

    Expresses gRNA targeting CDKN1A (p21) and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLIK-NFLAG-hFUS

    Plasmid

    #50487

    Purpose

    Produces lentivirus expressing human FUS with FLAG tag at its N-terminus by co-transfection with psPAX2 and pMD2G

    Depositor

    Zissimos Mourelatos

    Article

    Nakaya et al RNA. 2013 Apr;19(4):498-509. doi: 10.126

    Insert

    FUS (FUS Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

12. sgSf3b1(T1)

    Plasmid

    #90424

    Purpose

    induces dsDNA break within mouse Sf3b1 gene for subsequent homology-directed recombination and coding sequence mutagenesis at codon 700

    Depositor

    Alex Minella

    Article

    Gupta et al Nucleic Acids Res. 2018 Nov 20. pii: 519

    Insert

    mus Sf3b1 gRNA (Sf3b1 Mouse)
    

    Use

    CRISPR; Guiderna encoding for gene editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. rd-his

    Plasmid

    #112911

    Purpose

    Express the PEST domain-replaced 48 kDa form of human dematin with a cleavable N-terminal GST tag and non-cleavable C-terminal 6-his tag

    Depositor

    C. James McKnight

    Article

    Chen et al J Biol Chem. 2013 Mar 22;288(12):8313-20

    Insert

    dematin (DMTN Human)
    

    Use

    Tags

    6-his tag, Glutathione-S-Transferase, and preciss…

    Expression

    Bacterial

    Mutation

    89KSTSPPPSPEVWAD102 was replaced with 89KAAAGGGAG…

    Promoter

    T7

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS10-ribE-dddAI-FLAG

    Plasmid

    #186562

    Purpose

    Regulated expression in Firmicutes of the DddA immunity determinant with a C-terminal fusion to a FLAG epitope (DddAI-FLAG)

    Depositor

    Joseph Mougous

    Article

    Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

    Insert

    dddAI
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    pSpac(hy)

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-dCas13-M3M14nes

    Plasmid

    #155367

    Purpose

    Targeted m6A RNA methylation in mammalian cells

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A

    Promoter

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. AOI-WT-Cas9-sg-mouse Ezh2-E10-GFP

    Plasmid

    #91879

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Ezh2 (Ezh2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

17. AOI-WT-Cas9-sg-mouse Ezh2-E18-GFP

    Plasmid

    #91880

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Ezh2 (Ezh2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

18. sgALK(A)

    Plasmid

    #85562

    Purpose

    Expresses sgRNA targeting human ALK around codon F1174

    Depositor

    Luca Grumolato

    Article

    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

    Insert

    anaplastic lymphoma receptor tyrosine kinase (ALK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

19. sgALK(B)

    Plasmid

    #85563

    Purpose

    Expresses sgRNA targeting human ALK around codon F1174

    Depositor

    Luca Grumolato

    Article

    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

    Insert

    anaplastic lymphoma receptor tyrosine kinase (ALK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

20. PX459 mDnd1#1

    Plasmid

    #106345

    Purpose

    small guide RNA #1 against RRM-coding region of Dnd1 gene locus

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    Dnd1 (Dnd1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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