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ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pGPD-crtI-tNOS-pXYL-crtYB-tNOS+ku70 insD (SBE145)

Plasmid

#195047

Purpose

cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters

Depositor

Petri-Jaan Lahtvee

Publication

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Inserts

crtE
crtI
crtYB

Expression

Yeast

Promoter

pADH2, pGPD, and pXYL

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+ pXYL-G418-tNOS-pXYL-crtE-tNOS-pGPD-crtI-tNOS-pADH2-crtYB-tNOS+ku70 insD (SBE144)

Plasmid

#195046

Purpose

cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters

Depositor

Petri-Jaan Lahtvee

Publication

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Inserts

crtE
crtI
crtYB

Expression

Yeast

Promoter

pADH2, pGPD, and pXYL

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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(F) pXYL (G) P1 (SBE216)

Plasmid

#195017

Purpose

promoter of the gene xylose reductase from R. toruloides for the position P2 (F/G)

Depositor

Petri-Jaan Lahtvee

Publication

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(F) pXYL (G)

Expression

Bacterial

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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(R) pXYL (D) P1 (SBE215)

Plasmid

#195016

Purpose

promoter of the gene xylose reductase from R. toruloides for the position P1 (R/D)

Depositor

Petri-Jaan Lahtvee

Publication

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(R) pXYL (D)

Expression

Bacterial

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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(B) NAT marker (R) (SBE173)

Plasmid

#195014

Purpose

nourseothricin (NAT) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tNOS

Depositor

Petri-Jaan Lahtvee

Publication

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

pXYL+NAT+tNOS

Expression

Bacterial

Promoter

pXYL

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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(B) G418 marker (R) (SBE 137)

Plasmid

#195011

Purpose

geneticin (G418) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tNOS

Depositor

Petri-Jaan Lahtvee

Publication

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

pXYL+G418+tNOS

Expression

Bacterial

Promoter

pXYL

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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(I) pGPD (J)_P3 (SBE111)

Plasmid

#194990

Purpose

promoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P3 position (I/J)

Depositor

Petri-Jaan Lahtvee

Publication

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(I) pGPD (J)

Expression

Bacterial

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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(S) insD_ku70 (Q) (SBE139)

Plasmid

#187627

Purpose

the insertional region downstream for the deletion of the KU70 gene in R. toruloides. Overhangs S/Q

Depositor

Petri-Jaan Lahtvee

Publication

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(S) insD KU70 (Q)

Expression

Bacterial

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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(P) insUP_ku70 (SBE138) (B)

Plasmid

#187626

Purpose

the insertional region upstream for the deletion of the KU70 gene in R. toruloides. Overhangs P/B

Depositor

Petri-Jaan Lahtvee

Publication

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(P) insUP KU70 (B)

Expression

Bacterial

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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BB3eH_AE

Plasmid

#98546

Purpose

empty BB3 for insertion and overexpression of 4 genes in P. pastoris

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Publication

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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BB3eH_AF

Plasmid

#98547

Purpose

empty BB3 for insertion and overexpression of 5 genes in P. pastoris

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Publication

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB3eN_14*

Plasmid

#98549

Purpose

empty BB3 for insertion and overexpression of 1 gene in P. pastoris

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Publication

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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BB3rN_AC

Plasmid

#98551

Purpose

empty BB3 for insertion and overexpression of 2 genes in P. pastoris

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Publication

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB3rN_AD

Plasmid

#98552

Purpose

empty BB3 for insertion and overexpression of 3 genes in P. pastoris

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Publication

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB3rN_AE

Plasmid

#98553

Purpose

empty BB3 for insertion and overexpression of 4 genes in P. pastoris

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Publication

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB3rN_AF

Plasmid

#98554

Purpose

empty BB3 for insertion and overexpression of 5 genes in P. pastoris

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Publication

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB3rN_AI

Plasmid

#98557

Purpose

empty BB3 for insertion and overexpression of 8 genes in P. pastoris

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Publication

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB3aK_AC

Plasmid

#98530

Purpose

empty BB3 for insertion and overexpression of 2 genes in P. pastoris

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Publication

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB3aK_AD

Plasmid

#98531

Purpose

empty BB3 for insertion and overexpression of 3 genes in P. pastoris

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Publication

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB3aK_AE

Plasmid

#98532

Purpose

empty BB3 for insertion and overexpression of 4 genes in P. pastoris

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Publication

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge