Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 3,529 results for: golden gate

Showing: 961 - 980 of 3529 results

  1. pGA-red-mini

    Plasmid
    #196338
    Purpose
    Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening
    Depositor
    Paola Branduardi
    Article
    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly backbone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196338-plasmid-map-sequence-id-393164

    Enlarge

  2. pGA-red-maxi

    Plasmid
    #196337
    Purpose
    Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening
    Depositor
    Paola Branduardi
    Article
    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly backbone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196337-plasmid-map-sequence-id-393160

    Enlarge

  3. pAV10

    Plasmid
    #63213
    Purpose
    pUC19 backbone (Amp), contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. NotI or FseI sites for TU digest.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    63213-plasmid-map-sequence-id-113915

    Enlarge

  4. pENTR_AtU6p_BsaI_ccdb_gRNA

    Plasmid
    #225986
    Purpose
    To make Gateway entry clone of CRISPR guide RNA under AtU6p
    Depositor
    Imran Siddiqi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 7, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    225986-plasmid-map-sequence-id-448026

    Enlarge

  5. pPSG804

    Plasmid
    #239534
    Purpose
    CRISPR plasmid with Cas9 and sgRNA targeting YDR514C locus in S. cerevisiae
    Depositor
    John Dueber
    Insert
    sgRNA_YDR514C
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239534-plasmid-map-sequence-id-479034

    Enlarge

  6. pPSG779

    Plasmid
    #239532
    Purpose
    CRISPR plasmid withCas9 and sgRNA targeting YBR197C locus in S. cerevisiae
    Depositor
    John Dueber
    Insert
    sgRNA_YBR197C
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239532-plasmid-map-sequence-id-478373

    Enlarge

  7. pJJB1434

    Plasmid
    #239494
    Purpose
    Express Idi1p and Erg20WW for synthesis of GPP in the peroxisome
    Depositor
    John Dueber
    Insert
    ScErg20p(F96W_N127W)-ePTS1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239494-plasmid-map-sequence-id-475871

    Enlarge

  8. pPSG803

    Plasmid
    #239533
    Purpose
    CRISPR plasmid with Cas9 and sgRNA targeting YMR206W locus in S. cerevisiae
    Depositor
    John Dueber
    Insert
    sgRNA_YMR206W
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239533-plasmid-map-sequence-id-475867

    Enlarge

  9. pBD016

    Plasmid
    #201307
    Purpose
    Sr27 as Golden Gate compatible MoClo CDS part
    Depositor
    Benjamin Schwessinger
    Insert
    Sr27
    Use
    Tags
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Promoter
    Available since
    Jan. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    201307-plasmid-map-sequence-id-410032

    Enlarge

  10. pPD1159 pLink 9

    Plasmid
    #139247
    Purpose
    Vector that holds the linker for circuits with 9 TUs
    Depositor
    Joshua Leonard
    Insert
    Linker9
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    139247-plasmid-map-sequence-id-325057

    Enlarge

  11. pPD1158 pLink 8

    Plasmid
    #139246
    Purpose
    Vector that holds the linker for circuits with 8 TUs
    Depositor
    Joshua Leonard
    Insert
    Linker8
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    139246-plasmid-map-sequence-id-325056

    Enlarge

  12. pPD1157 pLink 7

    Plasmid
    #139245
    Purpose
    Vector that holds the linker for circuits with 7 TUs
    Depositor
    Joshua Leonard
    Insert
    Linker7
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    139245-plasmid-map-sequence-id-325055

    Enlarge

  13. pPD1156 pLink 6

    Plasmid
    #139244
    Purpose
    Vector that holds the linker for circuits with 6 TUs
    Depositor
    Joshua Leonard
    Insert
    Linker6
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    139244-plasmid-map-sequence-id-325054

    Enlarge

  14. pPD1155 pLink 5

    Plasmid
    #139243
    Purpose
    Vector that holds the linker for circuits with 5 TUs
    Depositor
    Joshua Leonard
    Insert
    Linker5
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    139243-plasmid-map-sequence-id-325051

    Enlarge

  15. pPD1154 pLink 4

    Plasmid
    #139242
    Purpose
    Vector that holds the linker for circuits with 4 TUs
    Depositor
    Joshua Leonard
    Insert
    Linker4
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    139242-plasmid-map-sequence-id-325049

    Enlarge

  16. pPD1153 pLink 3

    Plasmid
    #139241
    Purpose
    Vector that holds the linker for circuits with 3 TUs
    Depositor
    Joshua Leonard
    Insert
    Linker3
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    139241-plasmid-map-sequence-id-325047

    Enlarge

  17. pPD1152 pLink 2

    Plasmid
    #139240
    Purpose
    Vector that holds the linker for circuits with 2 TUs
    Depositor
    Joshua Leonard
    Insert
    Linker2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    139240-plasmid-map-sequence-id-325023

    Enlarge

  18. pPD1151 pLink 1

    Plasmid
    #139239
    Purpose
    Vector that holds the linker for circuits with 1 TUs
    Depositor
    Joshua Leonard
    Insert
    Linker1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    139239-plasmid-map-sequence-id-325022

    Enlarge

  19. C9m

    Plasmid
    #120958
    Purpose
    MoClo golden gate assembly CD part for deCFP (cyan fluorescent protein) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    deCFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120958-plasmid-map-sequence-id-235973

    Enlarge

  20. C76m

    Plasmid
    #120988
    Purpose
    MoClo golden gate assembly CD part for KanR (kanamycin resistance protein). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    KanR
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120988-plasmid-map-sequence-id-236053

    Enlarge

Showing: 961 - 980 of 3529 results