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mCherry-miniSOG-Peroxisomes-2

Plasmid

#54804

Purpose

Localization: Peroxisomes, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

mCherry-miniSOG-Peroxisomes

Tags

Peroxisomal Targeting Signal 1 (PTS1; Ser-Lys-Leu…

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only
mCherry-miniSOG-RatLC3

Plasmid

#55095

Purpose

Localization: Microtubule-Binding Protein, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

LC3

Tags

mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
p3E-OSBP

Plasmid

#67677

Purpose

Multisite gateway entry clone for adding C-terminal fusions of OSBP

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

OSBP

Use

Gateway multisite 3' entry clone

Mutation

aa 87-185 of NP_002547.1

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-C-Src-7

Plasmid

#55086

Purpose

Localization: Membrane/Vesicles, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

C-Src

Tags

mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
mCherry-miniSOG-Paxillin-22

Plasmid

#55092

Purpose

Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Paxillin

Tags

mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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PA-mCherry-miniSOG-Peroxisomes-2

Plasmid

#54847

Purpose

Localization: Peroxisomes, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

PA-mCherry-miniSOG-Peroxisomes

Tags

Peroxisomal Targeting Signal 1 (PTS1; Ser-Lys-Leu…

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only
pGEX4T-2 GST-mouse Fis1 (ΔTM domain)

Plasmid

#252268

Purpose

Expression of GST-mouse Fis1 (ΔTM domain) in bacteria

Depositor

David Chan

Article

Nakajima et al Curr Biol. 2026 Mar 9;36(5):1205-1219.e8

Insert

mouse Fis1 (deltaTM domain) (Fis1 Mouse)

Tags

GST

Expression

Bacterial

Mutation

moue Fis1 1-121 amino acids

Promoter

tac

Available Since

Feb. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pQCXIP EGFP-mouse Fis1 (W40A)

Plasmid

#252263

Purpose

Retroviral expression of EGFP-mouse Fis1 (W40A)

Depositor

David Chan

Article

Nakajima et al Curr Biol. 2026 Mar 9;36(5):1205-1219.e8

Insert

mouse Fis1 (W40A) (Fis1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Mutating tryptophan 40 to alanine (W40A) in Fis1 …

Promoter

CMV

Available Since

Feb. 24, 2026

Availability

Academic Institutions and Nonprofits only

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pQCXIP EGFP-mouse Fis1 (V79A)

Plasmid

#252264

Purpose

Retroviral expression of EGFP-mouse Fis1 (V79A)

Depositor

David Chan

Article

Nakajima et al Curr Biol. 2026 Mar 9;36(5):1205-1219.e8

Insert

mouse Fis1 (V79A) (Fis1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Mutating valine 79 to alanine (V79A) in Fis1 resu…

Promoter

CMV

Available Since

Feb. 24, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6(C82A)-flag-tev-his

Plasmid

#227166

Purpose

For overexpression of CYP2A6 C82A mutant in mammalian cells and purification.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag and His6

Expression

Mammalian

Mutation

changed cysteine 82 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6(C439A)-flag-tev-his

Plasmid

#227167

Purpose

For overexpression of CYP2A6 C439A mutant in mammalian cells and purification.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag and His6

Expression

Mammalian

Mutation

changed cysteine 439 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-cyp-33e1-flag-tev-halo-his

Plasmid

#227151

Purpose

For T-REX experiments of cyp-33e1. A reactive electrophilic species (RES) Sensor.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

cyp-33e1 (cyp-33E1 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-aex-5-flag-tev-halo-his

Plasmid

#227152

Purpose

For T-REX experiments of aex-5.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

aex-5 (aex-5 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6-flag-tev-halo-his

Plasmid

#227154

Purpose

For T-REX experiments of CYP2A6. A reactive electrophilic species (RES) Sensor.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-mCGN-mCGNL1-Rod1-Myc

Plasmid

#236526

Purpose

For expression of chimeric CGN containing the Rod1 domain from CGNL1 (mouse) in mammalian cells

Depositor

Sandra Citi

Article

Flinois et al J Cell Sci. 2024 Mar 1;137(5):jcs260745.

Insert

CGN-CGNL1 (Cgn Mouse)

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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EYFP-Parkin

Plasmid

#210292

Purpose

mitophagy marker

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

EYFP-Parkin

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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EYFP-FKBP-N-WASP

Plasmid

#210302

Purpose

targeted actin nucleation

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

EYFP-FKBP-N-WASP

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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HyWnt3-ΔRep-D3:Luc

Plasmid

#192990

Purpose

Plasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D3)

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Wnt3 promoter

Expression

Mammalian

Mutation

partial deletion of the Wnt3 repressor element-D3

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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pminiT-mEmerald-MED1 donor

Plasmid

#197867

Purpose

Donor plasmid used for building mEmerald-MED1 knock-in mESCs

Depositor

James Zhe Liu

Article

Dong et al bioRxiv 2022.10.26.513917

Insert

CRSP210 (Med1 Mouse)

Use

Mouse Targeting

Tags

mEmerald

Promoter

NA

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pTij_sg_mmu_Med6_C_terminal

Plasmid

#197869

Purpose

CRISPR/Cas9/sgRNA plasmid for cutting Med6 C terminal and building MED6-mEmerald/Halo knock-in mESCs

Depositor

James Zhe Liu

Article

Dong et al bioRxiv 2022.10.26.513917

Insert

Med6 (Med6 Mouse)

Expression

Mammalian

Promoter

U6

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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