We narrowed to 25,036 results for: Nes

1. pZ149-pcDNA3-BhCas12b_v4

   Plasmid

   #122446

   Purpose

   Expresses BhCas12b_v4 in mammalian cells

   Depositor

   Feng Zhang

   Article

   Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

   Insert

   CMV-NLS-BhCas12b_v4-NLS-3xHA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. p458 VQR

   Plasmid

   #101727

   Purpose

   Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VQR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   VQR

   Promoter

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6a:sgRNA(tyr)

   Plasmid

   #64250

   Purpose

   expresses sgRNA(tyr) under U6a promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Insert

   U6a:sgRNA (tyr)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. MT-cdc20

   Plasmid

   #11593

   Depositor

   Marc Kirschner

   Article

   Pfleger et al Genes Dev. 2001 Sep 15. 15(18):2396-407.

   Insert

   cdc20 (CDC20 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTRE-GFP-NMHC II-A

   Plasmid

   #10844

   Depositor

   Robert Adelstein

   Article

   Wei et al Mol Biol Cell. 2000 Oct . 11(10):3617-27

   Insert

   NMHC II-A (MYH9 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMC-J6-dCas9-10XGP41-J9

   Plasmid

   #202005

   Purpose

   MoClo level 0 dCas9-10XGP41 coding sequence module

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

   Insert

   dCas9-10XGP41
   

   Use

   Synthetic Biology; Moclo cloning vector

   Tags

   GS linkers

   Expression

   Mutation

   Promoter

   Available Since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCBL101.1

   Plasmid

   #199724

   Purpose

   A pVS1-based T-DNA binary vector with NPTII as a plant selectable marker gene. BsaI site was removed.

   Depositor

   Kan Wang

   Article

   Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

   Insert

   Multiple Cloning Site, NPTII
   

   Use

   Tags

   Expression

   Plant

   Mutation

   "GAGACC" to "GATACC"

   Promoter

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMU-ACTFr

   Plasmid

   #61191

   Purpose

   Fluorescent reporter for F-actin AtFim-ABD2-mCherry expressed under AtUBQ10 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   AtFimbrin1-ABD
   

   Use

   Tags

   mCherry

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available Since

   Feb. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-Gal4

   Plasmid

   #53366

   Purpose

   full-length native Gal4 with stop in Gateway middle entry clone

   Depositor

   Joshua Bonkowsky

   Article

   Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

   Insert

   native (full-length) Gal4
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   no promoter

   Available Since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. NCD-GFP

    Plasmid

    #24507

    Depositor

    Ron Vale

    Article

    Case et al Cell. 1997 Sep 5. 90(5):959-66.

    Insert

    NCD (ncd Fly)
    

    Use

    Tags

    GFP and His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pH6HTC_PTBP1-Halo

    Plasmid

    #175302

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    PTBP1 (PTBP1 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMU-LEr

    Plasmid

    #61185

    Purpose

    Fluorescent reporter for late endosome mCherry-MtRAB5A2 expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    MtRAB5A2
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available Since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTRANS_260d

    Plasmid

    #91126

    Purpose

    Transformation, Type: T-DNA, Plant Selection: PvUbi2:bar, Viral Replication: none

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    PvUbi2

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. iRFP670-PIP5K1A

    Plasmid

    #202749

    Purpose

    expresses fluorescent protein-tagged enzyme

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    iRFP670:PIP5K1A (PIP5K1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRANS_100

    Plasmid

    #91198

    Purpose

    Transformation, Type: non-T-DNA, Plant Selection: none, Viral Replication: none

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHACK(Gal4)-DONR(T2A-LexAGAD)

    Plasmid

    #194769

    Purpose

    To insert T2A-LexAGAD into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-LexAGAD transgene.

    Depositor

    Chun Han

    Article

    Karuparti et al G3 (Bethesda). 2023 Mar 9;13(3):jkad003.

    Insert

    T2A-LexAGAD
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCFJ68 - Exp5605_Punc-122::GFP::unc-54utr

    Plasmid

    #19325

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Punc-122::GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Oct. 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. cavin-2-mEGFP

    Plasmid

    #27710

    Depositor

    Ari Helenius

    Article

    Hayer et al Traffic. 2010 Mar . 11(3):361-82.

    Insert

    cavin-2 (CAVIN2 Human)
    

    Use

    Tags

    monomeric EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC118-FLIP-Puro

    Plasmid

    #84538

    Purpose

    a mock vector for generating FLIP targeting vectors

    Depositor

    Bon-Kyoung Koo

    Article

    Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

    Insert

    FLIP - Puro
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

20. pAFi-dHdrA

    Plasmid

    #184907

    Purpose

    carrying CRISPR/dCas9 system for gene knockdown in Acidithiobacillus ferridurans

    Depositor

    Radhakrishnan Mahadevan

    Article

    Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

    Insert

    Cm-sgRNA-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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