We narrowed to 20,145 results for: Sele

Showing: 9801 - 9820 of 20145 results

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pAS_4xMx PylT_FLAG-Mx PylRS YA

Plasmid

#140012

Purpose

Plasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Publication

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)

Tags

FLAG

Expression

Mammalian

Mutation

Y126A

Promoter

EF1

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV 3xFLAG-LC3B2-GFP

Plasmid

#123109

Purpose

Expresses 3xFLAG-LC3B2-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3B2

Depositor

Robin Ketteler

Publication

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)

Tags

3xFLAG and EGFP

Expression

Mammalian

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP3-44

Plasmid

#91780

Purpose

Mammalian expression of low affinity calcium-sensing fluorescent protein

Depositor

Douglas Kim, GENIE Project

Publication

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

GCaMP3-44

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pTE4566

Plasmid

#88904

Purpose

Expresses MbCpf1 crRNA and inactive/dead, humanized MbCpf1 nuclease

Depositor

Ervin Welker

Publication

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

Mb crRNA
hMbCpf1(D986A)

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV and human U6

Available since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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pSc1

Plasmid

#80436

Purpose

Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell

Depositor

Ervin Welker

Publication

Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

Inserts

sp Cas9 gRNA
eGFP

Use

CRISPR

Expression

Mammalian

Promoter

CMV and U6

Available since

Aug. 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP-Drosha1-850

Plasmid

#62522

Purpose

C-terminal aa851-1374 of Drosha is deleted

Depositor

Bharat Ramratnam

Publication

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 851-1374 deleted (DROSHA Human)

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 851-1374

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
mTFP1-MAPTau-N-10

Plasmid

#55498

Purpose

Localization: Microtubules, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Publication

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

MAPTau (MAPT Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-HA-CAD S1900A

Plasmid

#46240

Purpose

FLAG-HA-CAD with S1900 mutated to alanine

Depositor

Brendan Manning

Publication

Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

Insert

carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Serine 1900 changed to Alanine (S1900A)

Promoter

CMV

Available since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA Myc-mMEKK2

Plasmid

#44158

Depositor

Xin Lin

Publication

Blonska et al Mol Cell Biol. 2004 Dec;24(24):10757-65.

Insert

Mekk2 (Map3k2 Mouse)

Tags

Myc

Expression

Mammalian

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only
PLK3 335-646-pEGFP

Plasmid

#23267

Depositor

Wei Dai

Publication

Yang et al Cancer Res. 2008 Jun 1. 68(11):4077-85.

Insert

PLK3 (PLK3 Human)

Tags

pEGFP

Expression

Mammalian

Mutation

aa 335-646

Available since

Dec. 20, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
p3F-Strep-mBCL10

Plasmid

#33314

Depositor

Vanessa Redecke

Publication

Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

Insert

Bcl10 (Bcl10 Mouse)

Tags

3xFLAG and StrepOne

Expression

Mammalian

Promoter

EF1a

Available since

Dec. 21, 2011

Availability

Academic Institutions and Nonprofits only
pCDNA3 10 mut B-Myb

Plasmid

#25966

Depositor

Rob Lewis

Publication

Johnson et al J Biol Chem. 1999 Dec 17. 274(51):36741-

Insert

B-Myb (Mybl2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

changed threonines 267, 408, 497, 519, 522 and 52…

Available since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only
pIRES2-eGFP-Ofd1-Myc-S75F

Plasmid

#24580

Depositor

Jeremy Reiter

Publication

Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

Insert

Ofd1 (Ofd1 Mouse)

Tags

Myc and eGFP

Expression

Mammalian

Mutation

serine 75 changed to phenylalanine (codon TCT cha…

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag GK

Plasmid

#20493

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

GK (GK Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-hBnvy

Plasmid

#18969

Depositor

Mariano Viapiano

Publication

Hu et al J Biol Chem. 2008 Sep 5;283(36):24848-59

Insert

Brevican uncleavable (BCAN Human)

Expression

Mammalian

Mutation

Mutation at the ADAMTS cleavage site ESE/SRG: S…

Available since

Dec. 12, 2008

Availability

Academic Institutions and Nonprofits only
Flag Snail 2A4E

Plasmid

#16224

Depositor

Mien-Chie Hung

Publication

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Tags

Flag

Expression

Mammalian

Mutation

2A4E: S97A, S101A, S108E, S112E, S116E, S120E

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
GRM1-DuET

Plasmid

#213300

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Publication

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

GRM1 (GRM1 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS-GFP(ATG mut)

Plasmid

#197885

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

Depositor

Kazuto Kobayashi

Publication

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Mutation

N/A

Promoter

CAG

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pXL-BacII-CAG-loxP-STOP-loxP-Venus

Plasmid

#170520

Purpose

Plasmid containing piggyBac ITRs flanking CAG, a loxP-STOP-loxP cassette and Venus (also see doi: 10.7554/eLife.63907 )

Depositor

Joshua Sanes

Publication

Yamagata et al Bio Protoc. 2021 Aug 5;11(15):e4105. doi

Insert

CAG-loxP-STOP-loxP-Venus

Expression

Mammalian

Promoter

CAG

Available since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Centrobin

Plasmid

#136829

Purpose

Mammalian expression of the centrosomal protein Centrombin N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Publication

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Centrobin (CNTROB Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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