We narrowed to 14,916 results for: mal.3

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pQE60 R130E wgMDH

Plasmid

#204267

Purpose

Bacterial expression of R130E wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

R130E

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Xpress-GBF1

Plasmid

#206417

Purpose

Mammalian expression of hGBF1

Depositor

Kazuhisa Nakayama

Article

Kawamoto et al Traffic. 2002 Jul;3(7):483-95. doi: 10.1

Insert

GBF1 (GBF1 Human)

Expression

Mammalian

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBVboostFGII WPRE 2A-CVB3

Plasmid

#202076

Purpose

Bacterial expression of 2A protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.

Depositor

Vesa Hytönen

Article

Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

Insert

CVB3 2A protease with HisTag

Tags

6xHis-tag

Expression

Bacterial, Insect, and Mamm…

Promoter

polh, CAG, T7

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pNL(CMV)Rep78-III/CAG/WPREΔU3

Plasmid

#190727

Purpose

Expression of AAV2 Rep78-III in mammalian cells

Depositor

Jakob Reiser

Article

Li et al Hum Gene Ther Methods. 2018 Jun;29(3):13

Insert

AAV2 Rep78-III

Use

Lentiviral

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1107_Tier3(SB)

Plasmid

#169634

Purpose

Tier-3 vector for stable integration of up to three cassettes via sleeping beauty transposase (5'ITR-A1-pA::A2-pA::A3-pA-3'ITR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Type

Empty backbone

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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Epac-S-H96

Plasmid

#170342

Purpose

CFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

Depositor

Kees Jalink

Article

van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.

Insert

CFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)

Expression

Mammalian

Mutation

Deletion of DEP domain, Catalytically dead T781A …

Promoter

CMV

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pDY153: Flag(SUZ12) WT HDR plasmid

Plasmid

#170793

Purpose

WT homology directed repair plasmid for performing genome-editing on the SUZ12 Locus

Depositor

Thomas Cech

Article

Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

Insert

SUZ12 (SUZ12 Human)

Expression

Mammalian

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pDY156: SUZ12 px330 sgRNA Cas9 plasmid

Plasmid

#170792

Purpose

This plasmid encodes Cas9 and a sgRNA that targets the SUZ12 locus; to be used with pDY153

Depositor

Thomas Cech

Article

Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

Insert

Cas9

Expression

Mammalian

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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49535-sgFMR1_E17A

Plasmid

#157782

Purpose

Expresses a sgRNA targeting stop codon of human FMR1 gene

Depositor

Xinyu Zhao

Article

Li et al Genome Res. 2020 Mar;30(3):361-374. doi:

Insert

FMR1 (FMR1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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RG6-BAP1_E11

Plasmid

#154025

Purpose

Bichromatic Fluorescent Splicing Reporter Minigene for Wild-Type BAP1 Exon 11

Depositor

Samuel Peña-Llopis

Article

Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:

Insert

BAP1 Exon 11 Fused to DsRed1 and eGFP (BAP1 Human)

Use

Splicing reporter

Tags

DsRed1, FLAG, SV40 NLS, and eGFP

Expression

Mammalian

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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RG6-BAP1_E7

Plasmid

#154023

Purpose

Bichromatic Fluorescent Splicing Reporter Minigene for Wild-Type BAP1 Exon 7

Depositor

Samuel Peña-Llopis

Article

Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:

Insert

BAP1 Exon 7 Fused to DsRed1 and eGFP (BAP1 Human)

Use

Splicing reporter

Tags

DsRed1, FLAG, SV40 NLS, and eGFP

Expression

Mammalian

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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49535-sgFMR1_E3B

Plasmid

#157783

Purpose

Expresses a sgRNA targeting the 3rd exon of human FMR1 gene

Depositor

Xinyu Zhao

Article

Li et al Genome Res. 2020 Mar;30(3):361-374. doi:

Insert

FMR1 (FMR1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4.24-Cd36-enhancer7

Plasmid

#138579

Purpose

Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17918617_17920400

Depositor

Lora Hooper

Article

Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

Insert

Cd36 Promoter (Cd36 Mouse)

Use

Luciferase

Promoter

Minimal promoter

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4.24-Cd36-enhancer6

Plasmid

#138578

Purpose

Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17898458_17899478

Depositor

Lora Hooper

Article

Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

Insert

Cd36 Promoter (Cd36 Mouse)

Use

Luciferase

Promoter

Minimal promoter

Available Since

May 27, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4.24-Cd36-enhancer3

Plasmid

#138575

Purpose

Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17848003_17849903

Depositor

Lora Hooper

Article

Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

Insert

Cd36 Promoter (Cd36 Mouse)

Use

Luciferase

Promoter

Minimal promoter

Available Since

May 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCFL-mPML-2CAMut-iB

Plasmid

#140286

Purpose

Expression of mouse PML Ca mutant

Depositor

Yusuke Miyanari

Article

Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

Insert

PML (Pml Mouse)

Expression

Mammalian

Mutation

CA mutant (RING finger mutant)

Promoter

CAG

Available Since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Nfat1

Plasmid

#139830

Purpose

doxycycline-inducible expression of mouse Nfat1 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Nfat1

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-CP110

Plasmid

#136851

Purpose

Mammalian expression of the centrosomal protein Cep110 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-CP110 (CCP110 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetD-SNAP-CP110

Plasmid

#136803

Purpose

Mammalian expression of the centrosomal protein Cep110 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-CP110 (CCP110 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-plk4

Plasmid

#136837

Purpose

Mammalian expression of the centrosomal protein plk4 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-plk4 (PLK4 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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