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pENTR223.1_mmDdx58 (closed)

Plasmid

#160107

Purpose

Expresses murine Ddx58 in mammalian cells

Depositor

Rizwan Haq

Publication

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Ddx58 (Rigi Mouse)

Use

Gateway Cloning

Expression

Mammalian

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pWPXL-p150

Plasmid

#160992

Purpose

Express fulllength of mouse p150 protein

Depositor

Zhiguo Zhang

Publication

Cheng et al Nucleic Acids Res. 2019 Dec 2;47(21):111

Insert

CHAF1A (Chaf1a Mouse)

Tags

GFP

Expression

Mammalian

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pSG019

Plasmid

#126502

Purpose

Pseudomonas aeruginosa disaggregase ClpG cloned as ClpG-6xHis

Depositor

Ute Romling

Publication

Lee et al Proc Natl Acad Sci U S A. 2018 Jan 9;115

Insert

disaggregase ClpG with 6xHis-tag

Tags

C-terminal 6xHis

Expression

Bacterial

Promoter

PBAD

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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10XUAS-flp-DD

Plasmid

#124777

Purpose

Expresses flippase fused with destabilizing domain (DD) downstream of a 10XUAS sequence.

Depositor

Jing-W Wang

Publication

Sethi et al Elife. 2017 Nov 15;6. pii: e30327. doi:

Insert

TMP-inducible destabilized FLP (FLP-DD)

Tags

DD

Expression

Insect

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiX2-PURO-shGFP

Plasmid

#61230

Purpose

Lentivirus expressing shRNA to GFP

Depositor

Reuben Shaw

Publication

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

GFP

Use

Lentiviral

Promoter

H1/TO

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
mEos2-Keratin-17

Plasmid

#57392

Purpose

Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Publication

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Keratin (KRT18 Human)

Tags

mEos2

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pAM2314

Plasmid

#40246

Depositor

Susan Golden

Publication

Ditty et al Microbiology. 2005 Aug;151(Pt 8):2605-13

Insert

Polylinker

Use

Cyanobacteria cloning vector

Promoter

n/a

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pFBXC3GH

Plasmid

#49051

Purpose

FX cloning insect cell expression vector

Depositor

Markus Gruetter

Publication

Weinert et al Sci Rep. 2015 Jun 4;5:10819. doi: 10.103

Type

Empty backbone

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Insect

Promoter

polH (polyhedrin)

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR223-PAK7

Plasmid

#23850

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PAK7 (PAK5 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-CKM

Plasmid

#23866

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CKM (CKM Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-TNNI3K

Plasmid

#23469

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TNNI3K (TNNI3K Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp7

Plasmid

#163591

Purpose

Expresses BMP-7 in mammalian cells

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp7 (Bmp7 Mouse)

Expression

Mammalian

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAV0661

Plasmid

#133476

Purpose

Expression in fission yeast from promoter pact1, integrated at ade6 locus

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(act1)-sfGFP-terminator(ScCYC1)

Expression

Yeast

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pET51b(+)-SNAP-CLIP

Plasmid

#127180

Purpose

Bacterial expression of the fused self-labeling tags SNAP-tag and CLIP-tag

Depositor

Kai Johnsson

Publication

Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do

Insert

SNAP-CLIP

Tags

HisTag and StrepTag

Expression

Bacterial

Promoter

T7

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-P2A-APEX2-ChBP

Plasmid

#108879

Purpose

Expression of APEX2 fused to a nanobody against mCherry (includes green cytoplasmic reporter)

Depositor

Rob Parton

Publication

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

EGFP-P2A-APEX2-ChBP

Expression

Mammalian

Available since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-HA ZNHIT3

Plasmid

#110953

Purpose

Transient expression of HA ZNHIT3

Depositor

David Sabatini

Publication

Wyant et al Science. 2018 May 18;360(6390):751-758.

Insert

ZNHIT3 (ZNHIT3 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pENTR221-Renilla

Plasmid

#79496

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Renilla Luciferase CDS

Depositor

Peter Jon Nelson

Publication

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

Renilla Luciferase

Use

Gateway Cloning

Promoter

no Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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lenti_Blast_10N barcode

Pooled library

#238547

Purpose

Lentiviral expression of a Blasticidin resistance cassette liked to a random 10N barcode

Depositor

Jonathan Weissman

Publication

Koblan et al Science. 2025 Jul 24:eadx3800. doi: 10.1

Expression

Mammalian

Use

Lentiviral

Available since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only
pSaPIPLC His

Plasmid

#173810

Purpose

Expression and purification of mature, His-tagged Staphylococcus aureus (strain FPR3757) phosphaatidyl inositol specific phospholipase C (PI-PLC) from E. coli BL21 CodonPlus (DE3) RIL cells

Depositor

Anne Gershenson, Mary F Roberts

Publication

Goldstein et al Biochemistry. 2012 Mar 27;51(12):2579-87

Insert

Staphylococcus aureus plc

Tags

6XHis

Expression

Bacterial

Mutation

none

Promoter

T7

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pQCXIZ GFP PODXL B WT

Plasmid

#202414

Purpose

Expression of GFP-tagged PODXL (isoform B) WT

Depositor

David Bryant

Publication

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

PODXL (PODXL Human)

Use

Lentiviral

Tags

GFP

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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