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pLV-EF1A-ZCCHV-594-684-Hygro

Plasmid

#238102

Purpose

Expression of structurally predicted WWE domain of ZCCHV with EGFP on C terminal

Depositor

Robert Sobol

Publication

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

ZCCHV

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 594-684 only

Promoter

EF1alpha

Available since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-RNF146-45-182-EGFP-Hygro

Plasmid

#238091

Purpose

Expression of structurally predicted WWE domain of RNF146 with EGFP on C terminal

Depositor

Robert Sobol

Publication

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

RNF146 (RNF146 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 45-182 only

Promoter

EF1alpha

Available since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-TRIP12-391-938-EGFP-Hygro

Plasmid

#238092

Purpose

Expression of structurally predicted WWE domain of TRIP12 with EGFP on C terminal

Depositor

Robert Sobol

Publication

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

TRIP12 (TRIP12 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 319-938 only

Promoter

EF1alpha

Available since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-DTX1-1-175-EGFP-Hygro

Plasmid

#238093

Purpose

Expression of structurally predicted WWE domain of DTX1 with EGFP on C terminal

Depositor

Robert Sobol

Publication

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

DTX1 (DTX1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-175 only

Promoter

EF1alpha

Available since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-DTX4-1-166-EGFP-Hygro

Plasmid

#238095

Purpose

Expression of structurally predicted WWE domain of DTX4 with EGFP on C terminal

Depositor

Robert Sobol

Publication

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

DTX4 (DTX4 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-166 only

Promoter

EF1alpha

Available since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-HUWE1-1503-1780_EGFP-Hygro

Plasmid

#238096

Purpose

Expression of structurally predicted WWE domain of HUWE1 with EGFP on C terminal

Depositor

Robert Sobol

Publication

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

HUWE1 (HUWE1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-166 only

Promoter

EF1alpha

Available since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pET-29_GSS-6xHis-SUMO-VidC-EGFP-FLAG

Plasmid

#241009

Purpose

VidC-EGFP construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

Depositor

Cole DeForest

Publication

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

GSS-6xHis-SUMO-VidC-EGFP-FLAG

Tags

FLAG-tag, His-tag, and SUMO-tag

Expression

Bacterial

Promoter

T7

Available since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pET-29_GSS-6xHis-SUMO-VidC-mCherry-FLAG

Plasmid

#241010

Purpose

VidC-mCherry construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

Depositor

Cole DeForest

Publication

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

GSS-6xHis-SUMO-VidC-mCherry-FLAG

Tags

FLAG-tag, His-tag, and SUMO-tag

Expression

Bacterial

Promoter

T7

Available since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pET-21_GSS-6xHis-SUMO-VidC-bla-FLAG

Plasmid

#241012

Purpose

VidC-bla construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

Depositor

Cole DeForest

Publication

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

GSS-6xHis-SUMO-VidC-bla-FLAG

Tags

FLAG-tag, His-tag, and SUMO-tag

Expression

Bacterial

Promoter

T7

Available since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pET-21_GSS-6xHis-SUMO-VidC-EGF-FLAG

Plasmid

#241011

Purpose

VidC-EGF construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

Depositor

Cole DeForest

Publication

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

GSS-6xHis-SUMO-VidC-EGF-FLAG

Tags

FLAG-tag, His-tag, and SUMO-tag

Expression

Bacterial

Promoter

T7

Available since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCAD183_gCAD_pET

Plasmid

#239119

Purpose

Gorilla ACOD1 in E.coli expression vector

Depositor

Konrad Buessow

Publication

Chen et al Sci Rep. 2023 Jun 26;13(1):10360. doi: 1

Insert

ACOD1

Tags

StrepTag-II and TEV site

Expression

Bacterial

Promoter

T7

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCAD123_hIrg1_N152K,M154I_pvp008

Plasmid

#239118

Purpose

hACOD1 with N152K and M154I mutations in E.coli expression vector

Depositor

Konrad Buessow

Publication

Chen et al Sci Rep. 2023 Jun 26;13(1):10360. doi: 1

Insert

cis-aconitate decarboxylase (ACOD1 Human)

Tags

StrepTag and TEV site

Expression

Bacterial

Mutation

Deleted amino acids 1-3 and 462-end. Mutated N152…

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCAD130_hIrg1_M154I_pvp008

Plasmid

#239117

Purpose

hACOD1 with M154I mutation in E.coli expression vector

Depositor

Konrad Buessow

Publication

Chen et al Sci Rep. 2023 Jun 26;13(1):10360. doi: 1

Insert

cis-aconitate decarboxylase (ACOD1 Human)

Tags

StrepTag-II

Expression

Bacterial

Mutation

M154I

Promoter

T7

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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MIGR1-U6gRNA2-Filler

Plasmid

#237401

Purpose

For the sequential insertion of two guide RNAs into two U6gRNA cassettes.

Depositor

Zuoming Sun

Publication

Zhong et al STAR Protoc. 2025 Jun 20;6(2):103831. do

Inserts

U6gRNA-1
U6gRNA-2
PGK-TagBFP

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

PGK promoter, U6 promoter, and U6, PGK

Available since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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pCAD182_gCAD_pCMV6-entry

Plasmid

#239122

Purpose

Gorilla ACOD1 in mammalian expression vector

Depositor

Konrad Buessow

Publication

Chen et al Sci Rep. 2023 Jun 26;13(1):10360. doi: 1

Insert

ACOD1

Tags

FLAG and myc

Expression

Mammalian

Promoter

CMV

Available since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HRP-TM

Plasmid

#231497

Purpose

Expressing HRP on the cell surface of mammalian cells

Depositor

Baljit Khakh

Publication

Wu et al Neuron. 2025 Jun 5:S0896-6273(25)00366-6

Insert

HRP

Tags

Igk, HA, Myc, PDGFR-beta transmembrane domain

Expression

Mammalian

Promoter

CMV

Available since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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NLS-Cherry-PCNA-P2A-Venus-E2F3

Plasmid

#236448

Purpose

insect expression of E2F3 for protein purification

Depositor

Michele Pagano

Publication

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F3 (E2f3 Mouse)

Expression

Insect

Available since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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NLS-Cherry-PCNA-P2A-Venus-E2F1

Plasmid

#236446

Purpose

insect expression of E2F1 for protein purification

Depositor

Michele Pagano

Publication

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F1 (E2F1 Human)

Expression

Insect

Available since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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NLS-Cherry-PCNA-P2A-Venus-E2F2

Plasmid

#236447

Purpose

insect expression of E2F2 for protein purification

Depositor

Michele Pagano

Publication

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F2 (E2F2 Human)

Expression

Insect

Available since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Dot1LCD-CatMut

Plasmid

#235591

Purpose

Dox-inducible expression of control catalytic-mutant Dot1l CD fused with scFV

Depositor

Jamie Hackett

Publication

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Dot1l (Dot1l Mouse)

Use

CRISPR

Mutation

GS163-164RC

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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