We narrowed to 15,730 results for: NOL

1. pPhiC31-actIIORF4

   Plasmid

   #190785

   Purpose

   The PhiC31-phage based integration vector containing the actII-ORF4 gene under the control of strong kasOp* promoter

   Depositor

   Jan Kormanec

   Article

   Novakova et al Appl Microbiol Biotechnol. 2022 Nov;106(

   Inserts

   T5 terminator
   actII-4
   kasOp*
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNB_EA_R1CiM_4EGFP_R3EGFPi2

   Plasmid

   #185968

   Purpose

   Molecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.

   Depositor

   Sangram Bagh

   Article

   Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

   Insert

   Molecular IMPLY gate
   

   Use

   Synthetic Biology

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. 2xNLS-NLP-cMyc AspCas12a

   Plasmid

   #182120

   Purpose

   pET21a protein expression vector for 2xNLS-NLP-cMyc AspCas12a in bacteria

   Depositor

   Scot Wolfe

   Article

   Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

   Insert

   2xNLS-NLP-cMyc AspCas12a
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIALD2HMGr

   Plasmid

   #185867

   Purpose

   Knocking out ALD6; expressing acetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP and L. reuteri EutE) and an NADH-preferring HMG-CoA reductase (Delftia acidovorans mvaA) in S. cerevisiae

   Depositor

   Claudia Vickers

   Article

   Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

   Insert

   ALD6(-125, 40)> PSk.GAL1>Da.mvaA>TPGK1-PADH1> Lr.pduP> TPDC1-PGAL2>Lr.EutE-ALD6(1054, 1749)
   

   Expression

   Yeast

   Mutation

   None

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNB_EA_R3tdTomatoi2

   Plasmid

   #185969

   Purpose

   Expresses tdTomato from PR promoter with optimized RBS (i2) in E.coli Dh5alpha cell. The construct is in reverse direction network brick.

   Depositor

   Sangram Bagh

   Article

   Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

   Insert

   Pr-i2rbs-tdTomato cassette in reverse direction in Network Brick
   

   Use

   Synthetic Biology

   Promoter

   PR

   Available Since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pv6_CBX7_Chromo-W35A_TAF3_Phd

   Plasmid

   #179401

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo(W35A) - TAF3_Phd

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   CBX7_Chromo(W35A) - TAF3_Phd
   

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Mutation

   Chromodomain W35A

   Promoter

   CAGGS

   Available Since

   Aug. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIG-742pUC19-tNGFR-P2A-Caspase8

   Plasmid

   #186099

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   CASPASE8
   

   Use

   CRISPR

   Mutation

   WT with tNGFR sequence

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-U6-UL29-U3-UL8

   Plasmid

   #166685

   Purpose

   To express gRNA expression cassette simultaneously targeting two genes: UL8 and UL29 (non-EGFP version).

   Depositor

   Yujia Cai

   Article

   Yin et al Nat Biotechnol. 2021 Jan 11. pii: 10.103

   Insert

   UL8 and UL29 gRNA (UL29, UL8 HSV-1)
   

   Use

   Lentiviral

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJV126

   Plasmid

   #124250

   Purpose

   In vitro transcription plasmid for T7 transcription of wildtype Her2 sequence targeted to the endosome. Linearize with SapI.

   Depositor

   Stanley Riddell

   Article

   Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92

   Insert

   Minigene containing Her2 sequences fused to endosomal targeting sequence of class I MHC
   

   Use

   In vitro transcription template

   Promoter

   T7

   Available Since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJV127

    Plasmid

    #124251

    Purpose

    In vitro transcription plasmid for T7 transcription of Her2 ITD sequence targeted to the endosome. Linearize with SapI.

    Depositor

    Stanley Riddell

    Article

    Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92

    Insert

    Minigene containing Her2 sequences fused to endosomal targeting sequence of class I MHC
    

    Use

    In vitro transcription template

    Promoter

    T7

    Available Since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJV128

    Plasmid

    #124252

    Purpose

    In vitro transcription plasmid for T7 transcription of KRAS WT sequence targeted to the endosome. Linearize with SapI.

    Depositor

    Stanley Riddell

    Article

    Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92

    Insert

    Minigene containing KRAS sequences fused to endosomal targeting sequence of class I MHC
    

    Use

    In vitro transcription template

    Promoter

    T7

    Available Since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJV129

    Plasmid

    #124253

    Purpose

    In vitro transcription plasmid for T7 transcription of KRAS G12V sequence targeted to the endosome. Linearize with SapI.

    Depositor

    Stanley Riddell

    Article

    Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92

    Insert

    Minigene containing KRAS sequences fused to endosomal targeting sequence of class I MHC
    

    Use

    In vitro transcription template

    Promoter

    T7

    Available Since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV_eA3A T31A-nCas9

    Plasmid

    #163538

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    eA3A T31A-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)eA3A T31A

    Available Since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV_UdgX-EE-UdgX-nCas9

    Plasmid

    #163564

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)

    Available Since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-BRCA1-RNF169UBD

    Plasmid

    #119009

    Purpose

    Mammalian expression of a fusion protein of human BRCA1 with the human RNF169 Ubiquitin binding domain and BFP expression

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    BRCA1 (BRCA1 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-BRCA1-Rad18UBD

    Plasmid

    #119008

    Purpose

    Mammalian expression of a fusion protein of human BRCA1 with human Rad18 Ubiquitin binding domain and BFP expression

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    BRCA1 (BRCA1 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLCHKO_HPRT1_exon3_1_As

    Plasmid

    #155057

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and AsCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of HPRT1 exon 3
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLCHKO_HPRT1_exon3_2_As

    Plasmid

    #155058

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and AsCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of HPRT1 exon 3
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLCHKO_HPRT1_exon2_2_As

    Plasmid

    #155056

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and AsCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of HPRT1 exon 2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLCHKO_HPRT1_exon2_1_As

    Plasmid

    #155055

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and AsCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of HPRT1 exon 2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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