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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 17,254 results for: REV

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Showing: 9881 - 9900 of 17254 results

  3. pEFEX3-A2aGH

    Plasmid
    #160545
    Purpose
    pYES2 derivative reporter plasmid encoding PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.
    Depositor
    Michelle O'Malley
    Article
    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:
    Insert
    PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)
    Expression
    Yeast
    Mutation
    N/A
    Available Since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    160545-plasmid-map-sequence-id-317846

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  4. pCFEX3-A2aGH

    Plasmid
    #160548
    Purpose
    pYC2/CT derivative reporter plasmid encoding PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.
    Depositor
    Michelle O'Malley
    Article
    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:
    Insert
    PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)
    Expression
    Yeast
    Mutation
    N/A
    Available Since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    160548-plasmid-map-sequence-id-317848

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  9. pGU06

    Plasmid
    #212148
    Purpose
    Plasmid expressing alanine-glyoxylate aminotransferase, glutamate-pyruvate aminotransferase and tyrosine aminotransferase under constitutive promoter PgyrA control.
    Depositor
    Kang Zhou
    Insert
    alanine-glyoxylate aminotransferase, glutamate-pyruvate aminotransferase, tyrosine aminotransferase (AGX1, alaA, tyrB Agx1: S. cerevisiae; alaA, tyrB: E. coli, Budding Yeast)
    Expression
    Bacterial
    Promoter
    PgyrA
    Available Since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212148-plasmid-map-sequence-id-420636

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  10. kanMX6-ins1

    Plasmid
    #195038
    Purpose
    pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tDEG1), allows genome modification without disruption of insertion neighboring genes by transcription interference
    Depositor
    Gloria Brar
    Article
    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75
    Insert
    KanR
    Use
    Yeast genomic targeting
    Tags
    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…
    Available Since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    195038-plasmid-map-sequence-id-384240

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  11. Fibronectin-human-mutated N-terminal (N), V1 and V2 Glycosylation sites

    Plasmid
    #120407
    Purpose
    enables eukaryotic expression of human plasma fibronectin in which all three O-glycosylation sites were mutated from threonine to serine
    Depositor
    Inaam Nakchbandi
    Article
    Sens et al J Bone Miner Res. 2017 Jan;32(1):70-81.
    Insert
    Fibronectin (FN1 Human)
    Expression
    Mammalian
    Mutation
    Contains complete variable region with mutations …
    Promoter
    CMV
    Available Since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    120407-plasmid-map-sequence-id-232461

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  13. pBY011-AMN1ko

    Plasmid
    #183099
    Purpose
    S. cerevisiae Sigma1278b AMN1 gene knock out plasmid with KanMX selection marker.
    Depositor
    Gabor Balazsi
    Article
    Guinn et al Commun Biol. 2022 Mar 31;5(1):292. doi:
    Insert
    AMN1 (left homology arm) - KanMX - AMN1 (right homology arm) (AMN1 Budding Yeast)
    Tags
    KanMX
    Expression
    Bacterial and Yeast
    Mutation
    only includes external sequences (504 bp each) as…
    Promoter
    URA3, TEF1
    Available Since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183099-plasmid-map-sequence-id-361230

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  14. B9-COMP-blac-flag-his

    Plasmid
    #111020
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    6-cysteine protein (B9) (PF3D7_0317100 )
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    111020-plasmid-map-sequence-id-224595

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  15. CPN60-COMP-blac-flag-his

    Plasmid
    #111004
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    CPN60 (PF3D7_1232100 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    111004-plasmid-map-sequence-id-224554

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  16. CHT1-COMP-blac-flag-his

    Plasmid
    #110995
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    chitinase (CHT1) (PF3D7_1252200 )
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    110995-plasmid-map-sequence-id-224486

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  17. PF3D7_1350600-COMP-blac-flag-his

    Plasmid
    #110968
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    PF3D7_1350600 (PF3D7_1350600 )
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    110968-plasmid-map-sequence-id-224245

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  18. GPR180-COMP-blac-flag-his

    Plasmid
    #110955
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    PF3D7_1213500 (PF3D7_1213500 )
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Sept. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    110955-plasmid-map-sequence-id-221974

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  19. pJTB115

    Plasmid
    #248909
    Purpose
    Expresses yeast Smk1 and Ssp2 fused to glutathione-S-transferase in bacteria
    Depositor
    Edward Winter
    Article
    Tio et al Mol Cell Biol. 2017 May 2;37(10):e00607-
    Inserts
    SMK1 Mitogen-activated Protein Kinase (SMK1 Sequence is from the SK1 isolate of S. cerevisiae and will differ by 1 non-synonomous/several synonymous changes, Budding Yeast)
    Ssp2 (SSP2 Sequence is from the SK1 isolate of S. cerevisiae and may differ by synonymous changes from S288C form, Budding Yeast)
    Tags
    glutathione-S-transferase
    Expression
    Bacterial
    Mutation
    NdeI and NcoI sites were removed for cloning purp…
    Promoter
    T7
    Available Since
    Jan. 16, 2026
    Availability
    Academic Institutions and Nonprofits only
    248909-plasmid-map-sequence-id-497710

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  20. PGL4.11-COL1A1double mutants

    Plasmid
    #230918
    Purpose
    Double mutation of TEAD and SMAD binding elements in COL1A1 promoter; check the promoter activity.
    Depositor
    Avi Rosenberg
    Article
    Yang et al Lab Invest. 2024 May;104(5):100336. doi:
    Insert
    Human CLO1A1 promoter including SMAD and TEAD mutant elements (COL1A1 Human)
    Use
    Luciferase
    Tags
    Luciferase-luc2p
    Expression
    Bacterial and Mammalian
    Mutation
    COL1A1 promoter with mutant SMAD and TEAD binding…
    Promoter
    COL1A1 with mutant SMAD and TEAD elements
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230918-plasmid-map-sequence-id-460669

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Showing: 9881 - 9900 of 17254 results