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pCas9n-Nanog-L

Plasmid

#122302

Purpose

Expresses sgRNA targeting mouse Nanog and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Publication

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Nanog (Nanog Synthetic)

Expression

Mammalian

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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Prham-M.AvaIII-p15A-aadA

Plasmid

#149342

Purpose

expresses M.AvaIII DNA methyltransferase (M.NsiI ortholog) in E. coli

Depositor

Ichiro Matsumura

Publication

Matsumura et al PeerJ. 2020 Sep 11;8:e9841. doi: 10.7717

Insert

M.AvaIII

Expression

Bacterial

Promoter

rhamnose

Available since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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pXF90

Plasmid

#139044

Purpose

MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

Depositor

Erik Griffin

Publication

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

halotag::HDEL (includes STOP codon, PATC introns)

Expression

Worm

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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DYNLRB2

Plasmid

#132534

Purpose

Human Rbl-2, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Publication

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNLRB2 (DYNLRB2 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCas9FnCpf1GG

Plasmid

#131013

Purpose

Backbone plasmid for generating CRISPR arrays for composite array utilized by SpCas9 and FnCas12a using CRATES. Contains a GFP-dropout cassette and two direct repeats.

Depositor

Chase Beisel

Publication

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Inserts

direct repeat of SpCas9
GFP expression cassette
direct repeat of FnCas12a

Use

CRISPR

Expression

Bacterial

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2(+)-XSmurf1C699A

Plasmid

#117245

Purpose

mRNA synthesis

Depositor

Gerald Thomsen

Publication

Zhu et al Nature. 1999 Aug 12. 400(6745):687-93.

Insert

Smurf1-C699A mutant

Use

Xenopus expression

Mutation

mutated cysteine 699 to alanine

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI161

Plasmid

#126211

Purpose

Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11 and expression in plant cell

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

ccdB Cassette (BsaI)
GFP11

Use

Binary, module 1

Expression

Plant

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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MRLC-DD-pIRES-EGFP-AP4-mito

Plasmid

#101287

Purpose

To define the role of Mena/VASP in tension-sensitive actin assembly

Depositor

Alpha Yap

Publication

Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

Insert

MRLC-DD and AP4-Mito

Expression

Mammalian

Promoter

CMV

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pIVT/Acrosin-GFP-GPI

Plasmid

#122141

Purpose

For making acrosin-GFP-GPI RNA

Depositor

Carmen Williams

Publication

Winuthayanon et al Elife. 2015 Dec 1;4:e10453. doi: 10.7554

Insert

acrosin-GFP-GPI

Use

For in vitro transcription

Promoter

T7

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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LI A-B phrpB

Plasmid

#104514

Purpose

LI promoter module

Depositor

Thomas Lahaye

Publication

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

hrpB promoter

Use

Cloning vector

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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LI A-B pEPS

Plasmid

#104515

Purpose

LI promoter module

Depositor

Thomas Lahaye

Publication

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

eps promoter

Use

Cloning vector

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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LI A-B pGala7

Plasmid

#104516

Purpose

LI promoter module

Depositor

Thomas Lahaye

Publication

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

Gala7 promoter

Use

Cloning vector

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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LI E-F Tfd

Plasmid

#104520

Purpose

LI terminator module

Depositor

Thomas Lahaye

Publication

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

Tfd terminator

Use

Cloning vector

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-clbH-C-A2-PCP

Plasmid

#114157

Purpose

Expresses ClbH-C-A2-PCP

Depositor

Emily Balskus

Publication

Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

Insert

clbH

Tags

6xHis-thrombin-T7 tag

Expression

Bacterial

Mutation

Only contains the C-A2-PCP domains of ClbH

Available since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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YCe2748 HC_Kan_5'ITR-PB_p1

Plasmid

#100700

Purpose

part designed to occupy position 1 of EMMA. Functional category: Inverted Terminal Repeat

Depositor

Yizhi Cai

Publication

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

Piggybac 5' ITR

Use

Synthetic Biology

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2750 HC_Kan_3'ITR-PB_p24

Plasmid

#100701

Purpose

part designed to occupy position 24 of EMMA. Functional category: Inverted Terminal Repeat

Depositor

Yizhi Cai

Publication

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

Piggybac 3' ITR

Use

Synthetic Biology

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pAM-PAT-ProCDKA;1 GW

Plasmid

#79750

Purpose

binary DEST vector for plant expression

Depositor

Nico Dissmeyer, Arp Schnittger

Publication

Nowack et al Nat Genet. 2006 Jan;38(1):63-7. Epub 200

Type

Empty backbone

Tags

none

Expression

Plant

Promoter

ProCDKA;1

Available since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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AP553‐1

Plasmid

#87240

Purpose

gtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)

Depositor

Geraldine Seydoux

Publication

Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12

Insert

gtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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B0015_DE

Plasmid

#66035

Purpose

MoClo Basic Part: Double terminator (B0010:B0012) [D:B0015:E]

Depositor

Douglas Densmore

Publication

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Double Terminator

Use

Synthetic Biology

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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B0015_DF

Plasmid

#66036

Purpose

MoClo Basic Part: Double terminator (B0010:B0012) [D:B0015:F]

Depositor

Douglas Densmore

Publication

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Double Terminator

Use

Synthetic Biology

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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