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We narrowed to 10,083 results for: pET

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Showing: 9921 - 9940 of 10083 results

  1. SUMO CRYGS 16-177 Q16C

    Plasmid
    #217673
    Purpose
    N-terminally truncated human gamma S crystallin residues 16-177 with Q16C mutation for native chemical ligation
    Depositor
    Larry David
    Insert
    CRYGS (CRYGS Human)
    Tags
    6XHis and SUMO
    Expression
    Bacterial
    Mutation
    Glutamine 16 changed to Cysteine, and amino acids…
    Promoter
    T7
    Available since
    May 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    217673-plasmid-map-sequence-id-433254

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  3. hTln1-R1R2-17b

    Plasmid
    #191427
    Purpose
    Expresses a variant of the human TLN1 R1R2 domains containing a 17AA insert into R2 (exon 17b) in bacteria
    Depositor
    Ben Goult
    Publication
    Gallego-Paez et al J Cell Biol. 2023 May 1;222(5):e20220901
    Insert
    Human Talin1 R1R2 domains (TLN1 Human)
    Tags
    His-tag, TEV cleavage site
    Expression
    Bacterial
    Mutation
    contains 17AA insertion from inclusion of exon 17b
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    191427-plasmid-map-sequence-id-380511

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  6. Δ1-60MEK1C222

    Plasmid
    #164641
    Purpose
    Bacterial expression plasmid for His6-ΔMEK1C222
    Depositor
    Ben Davis
    Publication
    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00
    Insert
    N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
    Tags
    His6-tag
    Expression
    Bacterial
    Mutation
    S222C/C277S/C376S; residues 1-60 at N-terminus we…
    Promoter
    T7
    Available since
    May 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    164641-plasmid-map-sequence-id-328776

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  7. Δ1-60MEK1C218

    Plasmid
    #164643
    Purpose
    Bacterial expression plasmid for His6-ΔMEK1C218
    Depositor
    Ben Davis
    Publication
    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00
    Insert
    N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
    Tags
    His6-tag
    Expression
    Bacterial
    Mutation
    S218C/C277S/C376S; residues 1-60 at N-terminus we…
    Promoter
    T7
    Available since
    May 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    164643-plasmid-map-sequence-id-329215

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  12. pNC2

    Plasmid
    #72438
    Purpose
    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1); NCAIR synthetase (EcPurK)
    Depositor
    T. J. Kappock, JoAnne Stubbe
    Publication
    Meyer et al Biochemistry. 1999 Mar 9;38(10):3012-8.
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Expression
    Bacterial
    Mutation
    Pro64 to Arg; Asp65 to His; Gln206 to Arg
    Promoter
    T7 and n/a
    Available since
    May 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72438-plasmid-map-sequence-id-140433

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  13. pJK599

    Plasmid
    #72524
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, and associated hypothetical protein (AaPurS, AaOrfY, AaPurQ, AaPurLH6)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    72524-plasmid-map-sequence-id-132609

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  14. pJK598

    Plasmid
    #72523
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with C-terminal His6 tag, and associated hypothetical protein (AaPurS, AaOrfY, AaPurQ, AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    72523-plasmid-map-sequence-id-132608

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  18. pcDNA5/FRT/TO_darkmCherry-TGWETWV-IRES-HaloTag7

    Plasmid
    #263106
    Purpose
    For mammalian expression of non-fluorescent (dark) mCherry C-terminally tagged with the TGWETWV peptide, and HaloTag7.
    Depositor
    Peter Dedecker
    Publication
    Denis et al bioRxiv 2025.10.28.685015
    Inserts
    dark mCherry
    HaloTag7
    Tags
    TGWETWV peptide
    Expression
    Mammalian
    Mutation
    mCherry with mutation Y72N, which inactivates the…
    Promoter
    CMV/TO
    Available since
    Oct. 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    263106-plasmid-map-sequence-id-524817

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  19. PssaB-sfGFP-mRuby2

    Plasmid
    #257838
    Purpose
    Transcriptional reporter for SPI-2 (PssaB-sfGFP - no degradation tag) with constitutive mRuby2
    Depositor
    Keara Lane
    Publication
    Spratt et al Current Biology, Volume 36, Issue 13, 20
    Inserts
    PssaB-BBa_B0034-sfGFP-L3S2P21
    SJM901-PET3a_RBS-mRuby2-Bba_B0015
    Use
    Golden gate (ecoflex)
    Expression
    Bacterial
    Promoter
    PssaB and SJM901
    Available since
    Aug. 18, 2026
    Availability
    Academic Institutions and Nonprofits only
    257838-plasmid-map-sequence-id-515746

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  20. PssaG-sfGFP-mRuby2

    Plasmid
    #257840
    Purpose
    Transcriptional reporter for SPI-2 (PssaG-sfGFP - no degradation tag) with constitutive mRuby2
    Depositor
    Keara Lane
    Publication
    Spratt et al Current Biology, Volume 36, Issue 13, 20
    Inserts
    PssaG-BBa_B0034-sfGFP-L3S2P21
    SJM901-PET3a_RBS-mRuby2-Bba_B0015
    Use
    Golden gate (ecoflex)
    Expression
    Bacterial
    Promoter
    SJM901 and ssaG
    Available since
    Aug. 18, 2026
    Availability
    Academic Institutions and Nonprofits only
    257840-plasmid-map-sequence-id-515747

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Showing: 9921 - 9940 of 10083 results