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Plasmid Kit#1000000037PurposeSets of specific constitutive bacterial promoters that vary in strength, followed by specific Bi-Cistronic Design elements varying in effectiveness to drive E. coli expression of a gene of interestDepositorAvailable sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only
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Yeast Secrete and Detect
Plasmid Kit#1000000166PurposeYeast Golden Gate cloning for protein secretion in Saccharomyces cerevisiae and Pichia pastoris. Methanol utilization (MUT) promoters, signal peptides, C-terminal tags, episomal and integrative.DepositorAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
MetClo DNA Assembly Kit
Plasmid Kit#1000000153PurposeMetClo DNA Assembly Kit allows modular, hierarchical assembly of DNA fragments using IIS restriction enzyme. Includes 3 E.coli strains for BsaI, BpiI and LguI-based MetClo assembly.DepositorAvailable sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
CRIMPkit
Plasmid Kit#1000000225PurposeThe CRISPR/Cas9 Insertional Mutagenesis Protocol (CRIMP) and toolkit (CRIMPkit) is a universal and highly efficient targeted insertional mutagenesis system designed for use in zebrafish.DepositorAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
openCIDAR MoClo Toolkit
Plasmid Kit#1000000227PurposeThis collection includes broad host range, pBBR1-based vectors compatible with the CIDAR MoClo cloning standard. These vectors replicate in many bacteria across Pseudomonadota.DepositorAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
4G-Cloning Plasmid Kit
Plasmid Kit#1000000252PurposeThe 4G (Golden-Gate-guided Gibson) cloning kit provides elements for rapid one-day assembly of multi-gene expression vectors in bacterial and eukaryotic expression hosts.DepositorAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
MXS Chaining Kit
Plasmid Kit#1000000064PurposeMXS-chaining system for assembly of constructs optimized for synthetic biology applications in mammalian systems. 14 different fluorescent proteins, 10 mammalian promoters/enhancers, 3 polyA signalsDepositorAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ubiquiton Human Kit
Plasmid Kit#1000000244PurposeThe Ubiquiton system allows the inducible, linkage-specific polyubiquitylation of a protein of interest, accommodating for linear (M1-), K48-, and K63-linkage.DepositorAvailable sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
MoClo Yeast Toolkit
Plasmid Kit#1000000061Purpose96 standardized parts used for bottom-up hierarchical assembly of single and multi-gene constructs for S. cerevisiae expression. Includes promoters, terminators, peptide tags, selection cassettesDepositorAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
MoClo Tool Kit
Plasmid Kit#1000000044PurposeEukaryotic multigene construct assembly in 3 steps using modular cloning (MoClo); Has 95 empty standardized genetic modules: promoters, 5' UTRs, signal peptides, coding sequences, and terminatorsDepositorAvailable sinceApril 8, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
MoClo CRISPR/Cas Toolkit for Plants
Plasmid Kit#1000000159PurposeThe MoClo CRISPR/Cas Toolkit for Plants includes 95 plasmids consisting of CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots and dicots.DepositorAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
CIDAR MoClo Extension, Volume I
Plasmid Kit#1000000161PurposeThis kit contains 110 plasmids designed to quickly and efficiently build a wide variety of gene expression constructs using the CIDAR-standard MoClo system.DepositorAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
Joint Modular Cloning (JMC) Toolkit
Plasmid Kit#1000000233PurposeThe Joint Modular Cloning (JMC) Toolkit includes a modular cloning chassis and set of functional parts for assembling plant transformation vectors.DepositorAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
CIDAR MoClo Parts Kit
Plasmid Kit#1000000059PurposeCIDAR Modular Cloning (MoClo) kit uses modular DNA parts and enhanced MoClo protocols to enable rapid one-pot, multipart assembly, combinatorial design, and expression tuning in E. coliDepositorAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
EcoFlex MoClo Kit
Plasmid Kit#1000000080PurposeGolden Gate kit for E. coli synthetic biology; consists of a modular library that uses MoClo assembly standard for a variety of uses, e.g. recombinant protein purification, cell-free circuit designDepositorAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Zinc Finger Consortium Expression Vector and Modular Assembly Kit
Plasmid Kit#1000000005PurposeCan be used to efficiently assemble zinc finger arrays via modular assembly (Sangamo, Barbas, and Toolgen). Also includes the bacterial two-hybrid (B2H) system used to screen arrays for activity.DepositorAvailable sinceJuly 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
Shen Lab DdCBE assembly kit
Plasmid Kit#1000000212PurposeThe DddA-derived cytosine base editor (DdCBE) assembly kit is a collection of 192 modules harboring repeat variable di-residue (RVD) arrays for any desired target.DepositorAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
FusX TALEN assembly system
Plasmid Kit#1000000063PurposeGolden Gate assembly of 15-16 RVD TALEN inserts in a single cloning step; designed to be used with high throughput roboticsDepositorAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Winslow Lab Retroviral Barcoding Library
Plasmid Kit#1000000097PurposeBarcoding library of retroviral plasmids with unique 6 nucleotide barcodes, for in vivo phenotypic screening of small-molecule libraries.DepositorAvailable sinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
Musunuru-Cowan TALEN Kit
Plasmid Kit#1000000034PurposeUsed to efficiently assemble TALEN constructs with custom repeat arrays, each containing 15 repeats. Contains pre-assembled trimer and tetramer RVD domains designed for rapid TALEN assembly.DepositorAvailable sinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDSM-de
Plasmid#127720PurposeLevel 1 destination vector for a transcription unit, pathway position 5. Clone a transcription unit in with BsaI.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes7/Imdes7
Plasmid#107115Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des7DepositorInsertcolEdes7/Imdes7
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes8/Imdes8
Plasmid#107116Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des8DepositorInsertcolEdes8/Imdes8
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes9/Imdes9
Plasmid#107117Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des9DepositorInsertcolEdes9/Imdes9
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes10/Imdes10
Plasmid#107118Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des10DepositorInsertcolEdes10/Imdes10
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes11/Imdes11
Plasmid#107119Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des11DepositorInsertcolEdes11/Imdes11
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes12/Imdes12
Plasmid#107120Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des12DepositorInsertcolEdes12/Imdes12
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes13/Imdes13
Plasmid#107121Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des13DepositorInsertcolEdes13/Imdes13
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes14/Imdes14
Plasmid#107122Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des14DepositorInsertcolEdes14/Imdes14
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes15/Imdes15
Plasmid#107123Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des15DepositorInsertcolEdes15/Imdes15
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes16/Imdes16
Plasmid#107124Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des16DepositorInsertcolEdes16/Imdes16
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes17/Imdes17
Plasmid#107125Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des17DepositorInsertcolEdes17/Imdes17
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes18/Imdes18
Plasmid#107126Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des18DepositorInsertcolEdes18/Imdes18
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes1/Imdes1
Plasmid#107108Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des1DepositorInsertcolEdes1/Imdes1
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes2/Imdes2
Plasmid#107109Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des2DepositorInsertcolEdes2/Imdes2
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes3/Imdes3
Plasmid#107110Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des3DepositorInsertcolEdes3/Imdes3
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes3.5/Imdes3.5
Plasmid#107111Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des3.5DepositorInsertcolEdes3.5/Imdes3.5
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes4/Imdes4
Plasmid#107112Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des4DepositorInsertcolEdes4/Imdes4
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes5/Imdes5
Plasmid#107113Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des5DepositorInsertcolEdes5/Imdes5
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes6/Imdes6
Plasmid#107114Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des6DepositorInsertcolEdes6/Imdes6
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only