We narrowed to 1,151 results for: ELP

1. pcDNA3.1-FLAG-Dicer-delPAZ

   Plasmid

   #51367

   Depositor

   Narry Kim

   Article

   Lee et al EMBO J. 2006 Feb 8;25(3):522-32. Epub 20

   Insert

   Dicer (DICER1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   From N terminus to PAZ domain deletion

   Promoter

   CMV

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQE80L-SpyCatcher-ELP-GFP

   Plasmid

   #69835

   Purpose

   Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do

   Insert

   SpyCatcher-ELP-GFP
   

   Use

   Tags

   6x His Tag, GFP, and TEV Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T5 promoter/lac operator element

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMS20: pHelper(PmcCAST)_PmcPSP1_ΔTnsD

   Plasmid

   #168153

   Purpose

   Inducible expression of PmcCAST proteins (except TnsD) with crRNA targeting PmcPSP1.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   PmcCAST minimal CRISPR array (with spacer for PmcPSP1)
   PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   PmcCAST Tns proteins (TnsAB and TnsC)
   PmcTniQ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMS1: pHelper(AvCAST)_AvPSP1

   Plasmid

   #168133

   Purpose

   Inducible expression of AvCAST proteins with crRNA targeting AvPSP1.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   AvCAST minimal CRISPR array (with spacer for AvPSP1)
   AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
   AvTnsD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMS18: pHelper(PmcCAST)_PmcPSP1

   Plasmid

   #168151

   Purpose

   Inducible expression of PmcCAST proteins with crRNA targeting PmcPSP1.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   PmcCAST minimal CRISPR array (with spacer for PmcPSP1)
   PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
   PmcTniQ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMS23: pHelper(PmcCAST)_entry_ΔTnsD

   Plasmid

   #168156

   Purpose

   Inducible expression of PmcCAST proteins (except TnsD). Two BsaI sites for spacer cloning.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   PmcCAST minimal CRISPR array
   PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   PmcCAST Tns proteins (TnsAB and TnsC)
   PmcTniQ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMS21: pHelper(PmcCAST)_entry

   Plasmid

   #168154

   Purpose

   Inducible expression of PmcCAST proteins. Two BsaI sites for spacer cloning.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   PmcCAST minimal CRISPR array
   PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
   PmcTniQ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMS3: pHelper(AvCAST)_AvPSP1_ΔTnsD

   Plasmid

   #168135

   Purpose

   Inducible expression of AvCAST proteins (except TnsD) with crRNA targeting AvPSP1.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   AvCAST minimal CRISPR array (with spacer for AvPSP1)
   AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMS6: pHelper(AvCAST)_entry_ΔTnsD

   Plasmid

   #168139

   Purpose

   Inducible expression of AvCAST proteins (except TnsD). Two BsaI sites for spacer cloning.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   AvCAST minimal CRISPR array
   AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMAL-c5X-ELP[V5A2G3-60]

    Plasmid

    #67009

    Purpose

    Expresses malE-ELP[V5A2G3-60] in E. coli

    Depositor

    Ashutosh Chilkoti

    Article

    Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

    Insert

    malE-ELP[V5A2G3-60]
    

    Use

    Tags

    His6 and malE-Factor Xa

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQE80L SpyTag-ELP-SpyTag DK

    Plasmid

    #112635

    Purpose

    SpyTag linked by an elastin-like polypeptide to non-reactive SpyTag DK

    Depositor

    Mark Howarth

    Article

    Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

    Insert

    SpyTag-ELP-SpyTag DK
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Contains central cysteine. Reactive aspartic acid…

    Promoter

    T5

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMS22: pHelper(PmcCAST)_entry_ΔTniQ

    Plasmid

    #168155

    Purpose

    Inducible expression of PmcCAST proteins (except TniQ). Two BsaI sites for spacer cloning.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    PmcCAST minimal CRISPR array
    PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMS19: pHelper(PmcCAST)_PmcPSP1_ΔTniQ

    Plasmid

    #168152

    Purpose

    Inducible expression of PmcCAST proteins (except TniQ) with crRNA targeting PmcPSP1.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    PmcCAST minimal CRISPR array (with spacer for PmcPSP1)
    PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMS5: pHelper(AvCAST)_entry_ΔTniQ

    Plasmid

    #168138

    Purpose

    Inducible expression of AvCAST proteins (except TniQ). Two BsaI sites for spacer cloning.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    AvCAST minimal CRISPR array
    AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    AvCAST Tns proteins (TnsA, TnsB and TnsC)
    AvTnsD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMS4: pHelper(AvCAST)_entry

    Plasmid

    #168137

    Purpose

    Inducible expression of AvCAST proteins. Two BsaI sites for spacer cloning.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    AvCAST minimal CRISPR array
    AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
    AvTnsD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMS2: pHelper(AvCAST)_AvPSP1_ΔTniQ

    Plasmid

    #168134

    Purpose

    Inducible expression of AvCAST proteins (except TniQ) with crRNA targeting AvPSP1.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    AvCAST minimal CRISPR array (with spacer for AvPSP1)
    AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    AvCAST Tns proteins (TnsA, TnsB and TnsC)
    AvTnsD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mini-pHelper-1.0

    Plasmid

    #230933

    Purpose

    The mini verison (8.4 kb) of Ad helper plasmid to provide E2A, E4orf6, and VA RNA for AAV production

    Depositor

    Qizhao Wang

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. rAAV2-retro helper

    Plasmid

    #81070

    Purpose

    retrograde AAV helper vector, carrying rep and modified capsid gene

    Depositor

    Alla Karpova, David Schaffer

    Article

    Tervo et al Neuron. 2016 Oct 6. pii: S0896-6273(16)3

    Inserts

    rep
    rAAV2-retro cap
    

    Use

    AAV and Unspecified; Helper vector

    Tags

    Expression

    Mutation

    Pro2Ala, possibly Asp613Gly and changes made to A…

    Promoter

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ELP-TEMP/pcDNA3.1(-)

    Plasmid

    #178434

    Purpose

    Genetically encoded ratiometric fluorescent thermometer

    Depositor

    Takeharu Nagai

    Article

    Vu et al Sci Rep. 2021 Aug 13;11(1):16519. doi: 1

    Insert

    ELP-TEMP
    

    Use

    Tags

    No tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Tn7 helper plasmid

    Plasmid

    #141161

    Purpose

    Temperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoter

    Depositor

    Shimon Bershtein

    Article

    Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

    Insert

    Tn7 transposon machinery
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Arabinose-inducible pBAD

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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