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Plasmid#177179PurposePEAR-GFP control plasmid with split EGFP and intact 5' splice siteDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBFv-UAS3-GFP1-10
Plasmid#138398PurposeExpresses split GFP fragment (GFP1-10)DepositorInsertGFP1-10
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_mNG2(11)_Clathrin light chain
Plasmid#82608PurposeExpresses mNG2(11) tagged clathrin light chain in mammalian cellsDepositorInsertmNG2(11)_Clathrin light chain
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHRm-GFP(1-10)-VP64-NLS
Plasmid#70228PurposeExpresses GFP(1-10)-VP64-NLS in mammalian cellsDepositorInsertGFP(1-10)-VP64-NLS
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-3xGFP11-mem-3xControlgRNA
Plasmid#224583PurposeUbiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.DepositorInsert3xGFP11
UseCRISPRTagsMembrane Localization SignalExpressionMutationCodon optimizedPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAT9651-BEAR-GFP
Plasmid#162989PurposeBEAR target plasmid with split EGFP and disrupted 5' splice siteDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACUH-GFP1-10 sec
Plasmid#172062PurposeUAS vector to express GFP1-10 in the ER lumenDepositorInsertGFP1-10 sec
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nucGFP11-3xPax7gRNA
Plasmid#224570PurposeUbiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.DepositorInsertGFP11-H2B
UseCRISPRTagsHistone H2B (nuclear localization)ExpressionMutationCodon optimizedPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-LgBiT-MYPT1(1-314)(KAKA)-Flag
Plasmid#224894PurposeMammalian expression of LgBiT-MYPT1(1--314)DepositorInsertLgBiT-MYPT1(1-314)KAKA-Flag
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
OMMGFP 1-10
Plasmid#164119PurposeExpresses the non-fluorescent GFP1-10 moiety on the cytosolic face of the OMMDepositorInsertOMMGFP 1-10
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACUH-GFP11x7-mCherry-α-tubulin
Plasmid#70218PurposeExpresses GFP11x7-mCherry-α-tubulin in insect cells. 5 a. a. linker lengths.DepositorInsertGFP11x7-mCherry-α-tubulin
UseTagsGFP11x7 and mCherryExpressionInsectMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Cas13d-T2A-GFP(1-10)
Plasmid#224566PurposeUbiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and split GFP(1-10) reporter.DepositorInsertCas13d-T2A-GFP(1-10)
UseCRISPRTagsT2A-split GFP(1-10)ExpressionMutationPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAT9650-BEAR-GFP-preedited
Plasmid#162992PurposeBEAR control plasmid with split EGFP and intact 5' splice siteDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACUH-GFP1-10 mitochondrial matrix
Plasmid#172063PurposeUAS vector to express GFP1-10 in the mitochondrial matrixDepositorInsertGFP1-10 mitochondrial matrix
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-3xGFP11-mem-3xPax7gRNA
Plasmid#224571PurposeUbiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.DepositorInsert3xGFP11
UseCRISPRTagsMembrane Localization SignalExpressionMutationCodon optimizedPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRabies-EGFP-rtTAn
Plasmid#172119PurposeExpression of EGFP and rtTA-InteinN in Rabies virus infected cellsDepositorInsertEGFP, rtTAn-InteinN
UseRabies virusTagsExpressionMutationPromoterCMVAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pfNL43-dE-EGFP
Plasmid#36865DepositorInsertfNL43-dE-EGFP
UseLentiviral and RetroviralTagsExpressionMammalianMutationinserted EGFP after 6350 nucleotide of original N…PromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
HJP-224-actin5C^Syn21Gal80^IVS-Syn21myr-tdTomato-10UAS-mCD8GFP-p10w
Plasmid#162481PurposeA bicistronic construct - FloxGal80 and UAS-mCD8GFPDepositorInsertCre80Tom-GFP
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
L2179-FRB-GPA-6-GFPS
Plasmid#222893PurposeBiosensor chain detecting rapamycin and responding with split GFP reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 6 GS linker, split GFP fragment small.DepositorInsertFRB-GPA-6GS-GFPS
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
EGFP-ST-P2A-pSC-CAAX
Plasmid#207637PurposeA plasmid encoding EGFP fused to SpyTag (ST) and photocaged SpyCatcher (pSC) localized to the cell membrane via CAAX tag.DepositorInsertEGFP-SpyTag-P2A-photocaged SpyCatcher-CAAX
UseTagsExpressionMammalianMutationAmber stop codon at SC's critical lysinePromoterCMVAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only