Addgene

We narrowed to 1,188 results for: Aspa

Showing: 981 - 1000 of 1188 results

Results per page:

Sort results by:

pSP64T acvr1ba*

Plasmid

#185409

Purpose

Constitutively active form of acvr1ba (Nodal receptor). For in vitro transcription (SP6)

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

acvr1ba (acvr1ba Zebrafish)

Use

In vitro synthesis of mrna

Tags

Expression

Mammalian

Mutation

changed Threonine 206 to Aspartic acid

Promoter

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-Rab8a-TN

Plasmid

#86080

Purpose

A bacterial expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus GST.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

hRab8A (RAB8A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Changed threonine 22 to aspargine

Promoter

tac

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
RF-118-UB-BRCA1

Plasmid

#78960

Purpose

Expression of the C-terminal fragment of BRCA1 that is endogenously generated by caspase cleavage in mammalian cells

Depositor

Richard Fahlman

Article

Xu et al J Biol Chem. 2012 Mar 2;287(10):7495-502

Insert

BRCA1 (BRCA1 Human)

Use

Tags

3x Flag and ubiquitin

Expression

Mammalian

Mutation

delta 1-1155

Promoter

CMV

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMALp2x-SCO5461(E164D)(43-204)

Plasmid

#42508

Depositor

Koji Okamoto

Article

Nakano et al Toxicon. 2012 Dec 2;63C:55-63. doi: 10.1

Insert

guanosine ADP-ribosyltransferase (SCO5461 Streptomyces coelicolor A3(2))

Use

Tags

maltose-binding protein

Expression

Bacterial

Mutation

Deleted amino acids 1-42 (nucleotide 1-126); chan…

Promoter

tac

Available since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
pTG-mTDGa.1

Plasmid

#81049

Purpose

bacterial expression of catalytically dead murine TDG

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TDG (Tdg Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

asparagine 151 changed to alanine

Promoter

T7

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CITED2-T166N

Plasmid

#47749

Purpose

Introduces a T166N point mutation in the CITED2 gene

Depositor

Shoumo Bhattacharya

Article

Chen et al PLoS One. 2012;7(10):e46256. doi: 10.137

Insert

CITED2 (CITED2 Human)

Use

Tags

Expression

Mammalian

Mutation

Threonine 166 to Asparagine

Promoter

CMV

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pRN3P-HA-Suv4-20h2mut

Plasmid

#86692

Purpose

For transcription of of Suv4-20h2mut mRNA preceded by an HA-tag in 5'

Depositor

Maria-Elena Torres-Padilla

Article

Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

Insert

histone-lysine N-methyltransferase KMT5C mutant (Kmt5c Mouse)

Use

Tags

HA

Expression

Mutation

Mutated asparagine 273 to cysteine 276 (NHDC) int…

Promoter

T3

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PHB2 S39D-mCherry

Plasmid

#232787

Purpose

Encodes an mCherry-tagged Prohibitin2/PHB2 constitutively phosphorylated on Ser39

Depositor

Giulia Bertolin

Article

Bertolin et al Life Sci Alliance. 2021 Apr 5;4(6):e2020

Insert

PHB2 (PHB2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Changed Serine 39 to Aspartate

Promoter

CMV

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Shim_AspRS_3212.91_TP_GFP_pK7fWG2_IVD_FP611

Plasmid

#228374

Purpose

Expresses N-term transit peptide from Sapria himalayana Aspartate aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 3212.91; determination of S. himalayana AspRS target localization

Depositor

Daniel Sloan

Article

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from S. himalayana AspRS evm.model.fragScaff scaffold 3212.91

Use

Tags

Green Florescent Protien

Expression

Plant

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGEX-parkin K161N

Plasmid

#227365

Purpose

For bacterial expression of parkin with a mutated pUbl/pUb binding site on RING0.

Depositor

Kalle Gehring

Article

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin K161N (PRKN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJExpress401-KDAC8 D101R

Plasmid

#224349

Purpose

Expresses human KDAC8 (HDAC8) D101R in E. coli

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

Insert

KDAC8 (HDAC8 Human)

Use

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Aspartate 101 mutated to arginine

Promoter

T5

Available since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pJExpress401-KDAC8 D101A

Plasmid

#224347

Purpose

Expresses human KDAC8 (HDAC8) D101A in E. coli

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

Insert

KDAC8 (HDAC8 Human)

Use

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Aspartate 101 mutated to alanine

Promoter

T5

Available since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA3-RIPK3[V458D][Q459D][V460D][G461D]-FLAG

Plasmid

#199327

Purpose

mutated RHIM motive (interaction active)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed V458, Q459,V460 and G461 to aspartic acid

Promoter

Available since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
DNMT2Δ191-237

Plasmid

#198382

Purpose

Bacterial expression plasmid for human DNMT2 deletion mutant; for binding assays

Depositor

Tanja Schirmeister

Article

Schwickert et al ACS Med Chem Lett. 2023 May 9;14(6):777-

Insert

DNMT2 (TRDMT1 Human)

Use

Tags

His-tag

Expression

Bacterial

Mutation

deleted amino acids 191-237

Promoter

T7

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Sandgrouse+R111Q–insD

Plasmid

#191229

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

Inserts

ATP1A1
ATP1B1

Use

Tags

Expression

Insect

Mutation

removed aspartic acid at 121 and replaced arginin…

Promoter

PH and p10

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS313_ATP3mut

Plasmid

#120256

Purpose

Expression of ATP3-67 in yeast

Depositor

Markus Ralser

Article

Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

Insert

ATP3 (ATP3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Isoleucine 304 changed to Asparagine; Alanine 307…

Promoter

ATP3

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET151-GST-PP-RPN13 (19-132) R104D

Plasmid

#125827

Purpose

Expresses GST-RPN13 (19-132) R104D in E.coli

Depositor

Chris Hill

Article

Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

Insert

RPN13 (ADRM1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

changed arginine 104 to aspartate

Promoter

T7

Available since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCu-GFP-Atg17(416) (L313D I314K)

Plasmid

#121409

Purpose

Expressing GFP-Atg17

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

CUP1p-GFP-ATG17 ORF-CYC1 terminator

Use

Tags

Expression

Yeast

Mutation

Leucine 313 to Aspartic acid; Isoleucine 314 to …

Promoter

CUP1 promoter

Available since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKP110 [pRS416-YBR139Wp-YBR139W(N163,242Q)-PA-ADH1t]

Plasmid

#106462

Purpose

Expresses Atg42/Ybr139w(N163,242Q) with a C-terminal PA tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(N163,242Q)

Use

Tags

PA

Expression

Bacterial and Yeast

Mutation

changed Asparagines at positions 163 and 242 to G…

Promoter

YBR139W

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pKP133 [pRS406-YBR139Wp-YBR139W(D415A)-GFP-ADH1t]

Plasmid

#106468

Purpose

Expresses Atg42/Ybr139w(D415A) with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(D415A)

Use

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Changed Aspartate 415 to Alanine

Promoter

YBR139W

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge