We narrowed to 269,596 results for: addgene

1. integrin beta6 Y762F pBabe puro

   Plasmid

   #13597

   Depositor

   Dean Sheppard

   Insert

   integrin beta 6 Y762F (ITGB6 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Y762F

   Promoter

   Available since

   Jan. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pAAVTRE3_tdTomato

   Plasmid

   #135178

   Purpose

   AAV expression of TRE(TRE3G)-driven, tdTomato expression for fluorescent labling

   Depositor

   Tetsuo Yamamori

   Article

   Abe et al Front Neuroanat. 2018 Oct 30;12:89. doi:

   Inserts

   tdTomato
   TRE3G
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   a variant of DsRed

   Promoter

   TRE3G

   Available since

   Jan. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. FOR human NANOS3 TALEN

   Plasmid

   #59722

   Purpose

   TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)

   Depositor

   Jacob Hanna

   Article

   Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

   Insert

   HD HD NG NH HD NG HD NG HD HD HD NG HD HD NI NG
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Dec. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

4. REV human NANOS3 TALEN

   Plasmid

   #59723

   Purpose

   TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)

   Depositor

   Jacob Hanna

   Article

   Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

   Insert

   NG NH HD HD HD NI HD HD NG NH NG NI NH NH HD NI
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Dec. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

5. VVT1

   Plasmid

   #65779

   Purpose

   The Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   SaCas9 gRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pL90-Nat-2xHO

   Plasmid

   #139069

   Purpose

   CRISPR/Cas9 engineering of the HO endonuclease gene with nourseothricin selection

   Depositor

   Gianni Liti

   Article

   Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

   Insert

   Two guide RNAs targeting the HO endonuclease
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   TDH3

   Available since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLG1-puro-sgATL1-5

   Plasmid

   #109007

   Purpose

   CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   ATL1-sgRNA #5 (ATL1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6 promoter

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330_gRNA_H19-DMR-2

   Plasmid

   #159930

   Purpose

   Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse H19-DMR (H19-icr Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, CBh

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330_gRNA_H19-DMR-1

   Plasmid

   #159929

   Purpose

   Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse H19-DMR (H19-icr Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, CBh

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLG1-puro-sgATL1-2

    Plasmid

    #109006

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL1-sgRNA #2 (ATL1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19

    Plasmid

    #50005

    Purpose

    pUC cloning vector

    Depositor

    Joachim Messing

    Article

    Norrander et al Gene. 1983 Dec;26(1):101-6.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    lac

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. mu-FLT3Lv1-pTRIP

    Plasmid

    #163519

    Purpose

    Used to construct lentivirus to express murine FLT3 ligand (variant-1) in mammalian cells

    Depositor

    Thomas Weber

    Insert

    murine-FLT3L variant 1 (Flt3l Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-MetGFP

    Plasmid

    #37560

    Depositor

    David Rimm

    Insert

    MET (MET Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

    Plasmid

    #118156

    Purpose

    Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-P2A-EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9_human_ADAR1_ccC

    Plasmid

    #158115

    Purpose

    deletion hADAR1

    Depositor

    Emanuela Felley-Bosco

    Insert

    Adar1 (ADAR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSpCas9_human_ADAR1_ccB

    Plasmid

    #158116

    Purpose

    deletion hADAR1

    Depositor

    Emanuela Felley-Bosco

    Insert

    Adar1 (ADAR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV pCAG-FLEX-mScarlet-WPRE

    Plasmid

    #99280

    Purpose

    Can be used to generate AAV virus that will express mScarlet in the presence of Cre

    Depositor

    Rylan Larsen

    Insert

    mScarlet
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CMV-FLEXfrt-SaCas9-U6-sgRosa26

    Plasmid

    #159915

    Purpose

    Mutagenesis of Rosa26

    Depositor

    Larry Zweifel

    Insert

    Rosa26 (Gt(ROSA)26Sor Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. 7TFP

    Plasmid

    #24308

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    7xTcf-FFluc // SV40-PuroR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNR4A1.1.0-gDNA

    Plasmid

    #112391

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR4A1

    Depositor

    Kevin White

    Insert

    NR4A1 (NR4A1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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