We narrowed to 1,101 results for: control gRNA

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pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

Plasmid

#129048

Purpose

inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA8 targeting human TSDR

Depositor

Julia K Polansky

Publication

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-hudTET1CD, SgRNA8 (huTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A), catalytic domain huTET1 inact…

Promoter

CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

Plasmid

#129041

Purpose

inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA1 targeting human TSDR

Depositor

Julia K Polansky

Publication

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-hudTET1CD, SgRNA1 (huTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A), catalytic domain huTET1 inact…

Promoter

CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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mU6-CR1-CRISPRiv2_hU6-CR3-NT-ctrl-guide_EF1a-PuroR-T2A-BFP

Pooled library

#197348

Purpose

To allow for genome-wide genetic modifier screening, the library has a second fixed non-targeting control guide.

Depositor

Rebecca Voorhees, Jonathan Weissman

Publication

Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

Species

Homo sapiens

Use

Lentiviral

Available since

May 25, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pLCV2-AAVS1(hU6-sg1-mU6-sg2)-Blast

Plasmid

#208343

Purpose

pLentiCRISPRv2-Blast backbone with two separate sgRNAs against AAVS1. Serves as a negative control by targeting a safe harbor locus.

Depositor

Peter S Nelson

Publication

Frank et al eLife 2025

Insert

AAVS1 (AAVS1 Human)

Use

CRISPR and Lentiviral

Promoter

hU6, mU6

Available since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-CRISPRv2-sgNTC13-hygro

Plasmid

#231988

Purpose

Knockout non-targeting control

Depositor

Brian Ruffell

Publication

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 and hygromycin resistance

Use

CRISPR and Lentiviral

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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AA290

Plasmid

#215949

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control cell surface

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

U6_v1; sgCD55_v4; trRNA_v4 [Sp]

Use

CRISPR; Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA286

Plasmid

#215948

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control cell surface

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

U6_v1; sgCD47_v6; trRNA_v4 [Sp]

Use

CRISPR; Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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sgCTRL-hygro

Plasmid

#199639

Purpose

sgRNA control; induces CAS9 cutting in an intergenic region

Depositor

William Hahn

Publication

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A

Use

Lentiviral

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLV.U6.gLacZ.Cas9-T2A-GFP

Plasmid

#170361

Purpose

Negative control plasmid used for Crispr/cas9 based disruption.

Depositor

Johan Jakobsson

Publication

Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

Insert

Cas9-T2A-GFP

Use

Lentiviral

Promoter

U6

Available since

June 27, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMCB320-sgNC.ACOCA

Plasmid

#169836

Purpose

Expresses a negative control sgRNA

Depositor

Laura Attardi

Publication

Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

Insert

safe harbor sgRNA

Use

CRISPR, Lentiviral, and Mouse Targeting

Expression

Mammalian

Promoter

mU6

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pKDcas9 delta cerulean

Plasmid

#230939

Purpose

non-targeting Reference Control; expresses mCerulean

Depositor

Andrew Griffiths

Publication

Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

Insert

dCas9

Use

CRISPR

Mutation

additional tetO

Available since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pKDcas9 delta mcherry

Plasmid

#230940

Purpose

non-targeting Reference Control; expresses mCherry

Depositor

Andrew Griffiths

Publication

Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

Insert

dCas9

Use

CRISPR

Mutation

additional tetO

Available since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pRSITEP-puro-shWRN2-C911

Plasmid

#125786

Purpose

a seed-matched control for pRSITEP-puro-shWRN2

Depositor

Francisca Vazquez

Publication

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shWRN2 (WRN Human)

Use

RNAi

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-U6-sgCTG-CMV-GFP

Plasmid

#216734

Purpose

AAV plasmid containing sgCTG (target sequence: (CUG)6) driven by a U6 promoter and eGFP under the control of the CMV promoter. Used for in vivo experiments.

Depositor

Vincent Dion

Publication

Alvaro et al bioRxiv

Insert

sgCTG

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6 and CMV

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-SwitchON-sgCh2-2

Plasmid

#199637

Purpose

Tamoxifen-inducible expression of sgRNA control targeting intergenic region

Depositor

William Hahn

Publication

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A

Use

Lentiviral

Available since

June 2, 2023

Availability

Academic Institutions and Nonprofits only

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LentiCas9-sgCh2-2-Blast

Plasmid

#199645

Purpose

Expresses Cas9 and sgRNA control guide targeting intergenic region

Depositor

William Hahn

Publication

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A

Use

Lentiviral

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSpyB-Tn7sg

Plasmid

#248694

Purpose

sgRNA expression vector - pBG35 promoter with Tn7sg negative control sgRNA

Depositor

Larry Halverson

Publication

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

Tn7sg

Expression

Bacterial

Promoter

BG35

Available since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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Geph UTR3m shRNA

Plasmid

#121071

Purpose

Negative control shRNA for plasmid 121070: shRNA targeting the 3'-untranslated region (UTR) of the gephyrin mRNA

Depositor

Angel de Blas

Publication

Yu et al Mol Cell Neurosci. 2007 Dec;36(4):484-50

Insert

GPHN shRNA (Gphn Rat)

Use

RNAi

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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plentiCRISPRv2 Scramble

Plasmid

#258450

Purpose

Control for CRISPR-mediated knock-out

Depositor

Matthew Kutys

Publication

White et al J Cell Biol. 2023 Dec 4;222(12):e2023030

Insert

Scramble sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available since

July 22, 2026

Availability

Academic Institutions and Nonprofits only

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csr-1_qa-2_dsimpα_bar

Plasmid

#251402

Purpose

Enables inducible expression of double-stranded RNA targeting importin α (NCU01249) under the control of the qa-2 promoter, with the expression cassette inserted at the csr-1 locus.

Depositor

Jay C Dunlap

Publication

Wang et al Genetics. 2026 May 18:iyag126. doi: 10.1

Insert

importin alpha

Use

RNAi

Available since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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