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pAAV-nEF-Coff/Fon-ChR2-mCherry

Plasmid

#137144

Purpose

Intersectional viral expression of ChR2-mCherry in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Publication

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has service

AAV8

Insert

Coff/Fon-ChR2-mCherry

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

H134R

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGP-CMV-jGCaMP7c variant 1513

Plasmid

#105320

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Publication

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Insert

jGCaMP7c variant 1513

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

CMV

Available since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGIPZ-PD-L1-EGFP

Plasmid

#120933

Purpose

Express PD-L1-EGFP fusion protein in mammalian cells

Depositor

Mien-Chie Hung

Publication

Yang et al Cell Res. 2018 Aug;28(8):862-864. doi: 1

Insert

PD-L1-EGFP

Expression

Bacterial

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pMXs-hLIN28A

Plasmid

#47902

Purpose

Expresses human LIN28A in mammalian cells using retrovirus system.

Depositor

Shinya Yamanaka

Publication

Tanabe et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

human LIN28A (LIN28A Human)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available since

Oct. 2, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAJ075_hsCRBN

Plasmid

#124214

Purpose

Insect cell expression vector for His6 tagged hsCRBN

Depositor

Eric Fischer

Publication

Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

Insert

Cereblon (CRBN Human)

Tags

His6-TEV

Expression

Insect

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP

Plasmid

#137141

Purpose

Intersectional viral expression of ChR2(ET/TC)-EYFP in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Publication

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has service

AAV8

Insert

Coff/Fon-ChR2(ET/TC)-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E123T, T159C

Promoter

nEF

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Human DNA Binding Domain-Focused CRISPR Knockout Library

Pooled library

#123334

Purpose

Designed for genomic deletion screening of transcription factors by targeting the DNA binding domains.

Depositor

Christopher Vakoc

Publication

Huang et al Genes Dev. 2018 Jul 1;32(13-14):915-928.

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pAAV-Ef1a-Coff/Fon-sRGECO

Plasmid

#137128

Purpose

Intersectional viral expression of sRGECO in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Publication

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has service

AAV8

Insert

Coff/Fon-sRGECO

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E217D

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-CAG-SomaGCaMP6f2 (AAV9)

Viral prep

#158757-AAV9

Purpose

Ready-to-use AAV9 particles produced from pAAV-CAG-SomaGCaMP6f2 (#158757). In addition to the viral particles, you will also receive purified pAAV-CAG-SomaGCaMP6f2 plasmid DNA. CAG-driven expression of soma-targeting GCaMP6f2. These AAV preparations are suitable purity for injection into animals.

Depositor

Edward Boyden

Publication

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Promoter

CAG promoter

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only
pSL0527 (pDonor)

Plasmid

#130634

Purpose

Encodes a mini-transposon derived from V. cholerae CAST system, with CmR cargo gene. Total transposon size = 977 bp.

Depositor

Samuel H. Sternberg

Publication

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchCAST donor DNA (Mini-Tn)

Use

CRISPR; Transposon

Expression

Bacterial

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMRE-Tn7-145

Plasmid

#118561

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Minitn7 delivery vector

Expression

Bacterial

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-hSyn1-mNeonGreen

Plasmid

#99135

Purpose

An AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the hSyn1 promoter

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mNeonGreen

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-Ef1a-Con/Foff 2.0-BFP

Plasmid

#137130

Purpose

Intersectional viral expression of BFP in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Publication

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has service

AAV8

Insert

Con/Foff 2.0-BFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCMV-PfV-Sapphire-IRES-DSRed

Plasmid

#116934

Purpose

Mammalian lenti vector for expression of the 40nm GEM and DSRed

Depositor

Liam Holt

Publication

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

Pfv

Use

Lentiviral

Tags

Sapphire

Promoter

CMV

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-CAG-tTA

Plasmid

#99118

Purpose

An AAV genome that ubiquitously expresses tTA from the CAG promoter

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Has service

AAV2

Insert

tTA

Use

AAV

Expression

Mammalian

Mutation

*(see below)

Promoter

CAG

Available since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mRuby2

Plasmid

#99123

Purpose

An AAV genome that ubiquitously expresses the fluorescent protein mRuby2 from the CAG promoter

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mRuby2

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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DRH313: FCK-CheRiff-eGFP

Plasmid

#51693

Purpose

Channelrhodopsin with improved sensitivity and minimal red light photocurrent

Depositor

Adam Cohen

Publication

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

CheRiff-eGFP

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

CaMKIIa

Available since

June 23, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pLX304-YAP1(S94A)

Plasmid

#59145

Purpose

YAP1 serine 94 o alanine mutants which disrupts binding to TEAD transcription factors

Depositor

William Hahn

Publication

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

S94A

Promoter

CMV

Available since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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DRH229: FCK-QuasAr1-mO2

Plasmid

#51629

Purpose

Fluorescent reporter for membrane voltage with improved brightness

Depositor

Adam Cohen

Publication

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

QuasAr1-mOrange

Use

Lentiviral

Tags

mOrange2

Expression

Mammalian

Promoter

CaMKIIa

Available since

June 23, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSR657

Plasmid

#65214

Purpose

Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cells

Depositor

Robert Kotin

Publication

Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

Inserts

modified AAV-2 rep78
modified AAV-2 cap

Use

AAV

Expression

Insect

Promoter

p10 and polyhedrin

Available since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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