We narrowed to 4,567 results for: organization Addgene

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pHR-PGK-ABI-dCas9-P2A-mcherry

Plasmid

#121513

Purpose

expression of AB1-fused-dcas9 plasmids

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

dCas9

Use

Lentiviral

Tags

AB1 and P2A-mcherry

Expression

Mammalian

Promoter

PGK

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-4031NES

Plasmid

#105241

Purpose

A Förster resonance energy transfer (FRET) biosensor for AMPK activity with cyan and yellow fluorescent protein.

Depositor

Michiyuki Matsuda

Article

Konagaya et al Cell Rep. 2017 Nov 28;21(9):2628-2638. d

Insert

AMPK-EV

Expression

Mammalian

Promoter

CAGGS

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD miniSOG

Plasmid

#91774

Purpose

Bacterial expression of a fluorescent protein, mini Singlet Oxygen Generator (miniSOG)

Depositor

Erik Rodriguez, Roger Tsien

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

mini Singlet Oxygen Generator

Tags

Hexa-Histidine tag

Expression

Bacterial

Promoter

araBAD

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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CTB

Plasmid

#141166

Purpose

Expresses recombinant Cholera Toxin B Subunit

Depositor

Wayne Lencer

Article

Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

Insert

CTB

Expression

Bacterial

Mutation

No secretion signal

Promoter

T7

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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pSEMS-Halo7Tag-hFis

Plasmid

#111136

Purpose

hFis with selflabeling tag, e.g. for fluorescence microscopy

Depositor

Karin Busch

Article

Appelhans et al Nano Lett. 2012 Feb 8;12(2):610-6. doi:

Insert

human fission factor1 (FIS1 Human)

Tags

Halo7-tag

Expression

Mammalian

Mutation

*

Promoter

CMV

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pETduet+sfGFP-TetR-p300HAT

Plasmid

#157795

Purpose

Expression of sfGFP-TetR-p300HAT fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

sfGFP-TetR-p300 (EP300 Human)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-Cdc42BPB

Plasmid

#50759

Purpose

expression of EGFP tagged Cdc42BPB

Depositor

Naoki Mochizuki

Article

Ando et al J Cell Biol. 2013 Sep 16;202(6):901-16.

Insert

Cdc42BPB (CDC42BPB Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pSEMS-Tom20-Halo7Tag

Plasmid

#111135

Purpose

Tom20 with selflabeling tag, e.g. for fluorescence microscopy

Depositor

Karin Busch

Article

Appelhans et al Nano Lett. 2012 Feb 8;12(2):610-6. doi:

Insert

Receptor of tranlocase of outer mitochondrial membrane Tom20 (TOMM20 Human)

Tags

Halo7 Tag

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+(Zeo)-FLAG-muGFP

Plasmid

#166726

Purpose

Expresses FLAG-tagged monomeric ultrastable GFP (muGFP) in mammalian cells

Depositor

Daniel Scott

Article

Scott et al Sci Rep. 2018 Jan 12;8(1):667. doi: 10.1

Insert

muGFP

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pJJR82

Plasmid

#75027

Purpose

EGFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Mike Boxem

Article

Ramalho et al Development. 2020 Jul 22;147(14):dev1880

Insert

EGFP^SEC^3xFlag

Use

CRISPR

Tags

3xFLAG and C. elegans codon optimized EGFP

Expression

Worm

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ML1Nmut_in_pEGFP-C1

Plasmid

#67796

Purpose

Mammalian expression vector driving Mouse ML1Nwt (2xML1N domain 7Q mutant version) fused to EGFP

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

ML1N*2(7Q)-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pJJR83

Plasmid

#75028

Purpose

mCherry^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Mike Boxem

Article

Ramalho et al Development. 2020 Jul 22;147(14):dev1880

Insert

mCherry^SEC^3xFlag

Use

CRISPR

Tags

3xFLAG and C. elegans codon optimized mCherry

Expression

Worm

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAM-DCA-muGFP

Plasmid

#166727

Purpose

AAV, encodes monomeric ultrastable GFP (muGFP)

Depositor

Daniel Scott

Article

Scott et al Sci Rep. 2018 Jan 12;8(1):667. doi: 10.1

Insert

muGFP

Use

AAV

Promoter

Chicken Beta Actin

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FKBP-ICUE3-NLS

Plasmid

#138255

Purpose

FRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.

Depositor

Jin Zhang

Article

Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89

Insert

FKBP-ICUE3-NLS

Tags

FKBP, Nuclear localization signal (NLS), cpVenus[…

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO CBX1-APEX2

Plasmid

#129642

Purpose

Express heterochromatin-localized CBX1-APEX2 fusion in mammalian cells

Depositor

Nicholas Ingolia

Article

Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

Insert

CBX1 (CBX1 Human)

Tags

1xFLAG and APEX2

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-Lifeact-mRuby

Plasmid

#194339

Purpose

Expresses Lifeact with mRuby which labels F-actin in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Article

Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

Insert

Lifeact

Tags

mRuby

Expression

Mammalian

Promoter

CMV IE94

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-DCX-2A-mCherry

Plasmid

#118290

Purpose

Expresses human DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells.

Depositor

Vance Lemmon

Article

Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

Insert

doublecortin (DCX Human)

Use

AAV

Tags

2A peptide and mCherry

Expression

Mammalian

Promoter

CMV with beta global intron

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-hSHH

Plasmid

#179586

Purpose

ePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette with SHH expression (Red module-dsRed/Green module-NeonGreen T2A SHH)

Depositor

Ali Hemmati Brivanlou

Article

De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

Insert

NLS-NG-T2A-hSHH

Use

Piggybac

Tags

NeonGreen

Promoter

TRE

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pUBC_HA_PP7-mVenus

Plasmid

#111268

Purpose

Transient expression of non-aggregating delta-FG mutant, fused to mVenus in mammalian cells, under a hUBC promoter, HA tag

Depositor

John Rinn

Article

Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

Insert

PP7-mVenus

Use

Synthetic Biology

Tags

HA, SV40 NLS, and mVenus

Expression

Mammalian

Mutation

PP7: C68S, S69G [delta]70-75 (non-aggregating)

Promoter

hUBC

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-ML1N*2

Plasmid

#67678

Purpose

Multisite gateway entry clone for adding C-terminal fusions of ML1N*2

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

ML1N*2

Use

Gateway multisite 3' entry clone

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

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