We narrowed to 2,547 results for: organization Addgene

1. AICSDP-4:TUBA1B-mEGFP

   Plasmid

   #87421

   Purpose

   Homology arms and linker-mEGFP sequence for N-terminus (second exon) tagging of human TUBA1B

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   TUBA1B Homology Arms with mEGFP-linker (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   March 15, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCAG-SpyTag-Flag-SRSF1-V5-mCherry

   Plasmid

   #235089

   Purpose

   SRSF1 mCherry fusion protein without MCP domain

   Depositor

   Mitchell Guttman

   Article

   Bhat et al Nature. 2024 May;629(8014):1165-1173. do

   Insert

   pCAG-SpyTag-Flag-SRSF1-V5-mCherry (SRSF1 Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-MCP-SRSF1-V5-mCherry

   Plasmid

   #235088

   Purpose

   SRSF1 mCherry fusion protein with MCP domain

   Depositor

   Mitchell Guttman

   Article

   Bhat et al Nature. 2024 May;629(8014):1165-1173. do

   Insert

   pCAG-MCP-SRSF1-V5-mCherry (SRSF1 Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti GW V5 Eco/Dam hum LaminB1

   Plasmid

   #182671

   Purpose

   Eukaryotic expression vector with E.Coli Dam methylation fused to human Lamin B1. Used in DamID experiments to methylate DNA that interacts in the proximity of Lamin B1.

   Depositor

   Bas van Steensel

   Article

   Guelen et al Nature. 2008 Jun 12;453(7197):948-51. do

   Insert

   Lamin B1 (LMNB1 Human)
   

   Use

   Lentiviral

   Tags

   E. Coli Dam and V5

   Expression

   Mammalian

   Promoter

   HSP

   Available Since

   April 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. AICSDP-13:FBL-mEGFP

   Plasmid

   #87427

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human FBL

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   FBL Homology Arms with linker-mEGFP (FBL Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   March 15, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pCAG-SpyTag-Flag-Lbr-V5-mCherry

   Plasmid

   #235094

   Purpose

   Lbr mCherry fusion protein without MCP domain

   Depositor

   Mitchell Guttman

   Article

   Bhat et al Nature. 2024 May;629(8014):1165-1173. do

   Insert

   pCAG-SpyTag-Flag-Lbr-V5-mCherry (LBR Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHOT-P2Y2R

   Plasmid

   #159108

   Purpose

   entiviral vector encoding P2Y purinoceptor 2 (P2Y2R), a high affinity G-protein coupled receptor that mobilizes Ca2+ from intracellular stores upon binding extracellular ATP.48,49 By using the Gibson

   Depositor

   Fabio Mammano

   Article

   Mazzarda et al Lab Chip. 2020 Jul 23. doi: 10.1039/d0lc

   Insert

   P2Y2R
   

   Use

   Lentiviral

   Promoter

   UBC

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Tol2-pA-GI-TagRFP-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP

   Plasmid

   #135812

   Purpose

   This plasmid can be used to express independently eGFP and TagRFP from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.

   Depositor

   Nadia Mercader Huber

   Article

   Sanz-Morejon et al Cell Rep. 2019 Jul 30;28(5):1296-1306.e6

   Insert

   TagRFP
   

   Use

   Zebrafish

   Promoter

   UAS

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEN-L1-K2-L2

   Plasmid

   #80684

   Purpose

   Gateway Entry vector containing a temperature-sensitive mouse DHFR

   Depositor

   Nico Dissmeyer

   Article

   Faden et al Nat Commun. 2016 Jul 22;7:12202. doi: 10

   Inserts

   DHFR (Dhfr Mouse)
   DHFR (Dhfr Mouse, Mustard Weed)
   

   Use

   Synthetic Biology

   Tags

   3xHA

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   mouse DHFR with N-terminal Ubiquitin

   Available Since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Tbg-CES2A-ΔC-S227A

    Plasmid

    #200560

    Purpose

    Secreted mouse CES2A S227A mutant driven by heptaocyte-specific promoter in AAV viral vector

    Depositor

    Jonathan Long

    Article

    Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

    Insert

    Mouse Carboxylesterase 2A S227A mutant delta C-terminal (Ces2a Mouse)
    

    Use

    AAV

    Tags

    Flag

    Promoter

    CMV promoter

    Available Since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUAS-hhN-HA

    Plasmid

    #240236

    Purpose

    Expression of hhN-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

    Depositor

    Justin Bosch, Norbert Perrimon

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Insert

    hedgehog N-terminal fragment (hh Fly)
    

    Tags

    HA-spGFP11

    Expression

    Insect

    Mutation

    aa 1-257

    Promoter

    UAS

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR_hhN-HA

    Plasmid

    #240225

    Purpose

    Gateway entry clone with N-terminal hedgehog (hhN) tagged with HA (contains stop codon)

    Depositor

    Justin Bosch, Norbert Perrimon

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Insert

    hedgehog N-terminal fragment (hh Fly)
    

    Use

    Gateway shuttle vector

    Tags

    HA-spGFP11

    Mutation

    aa 1-257

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-MCP-Lbr-V5-mCherry

    Plasmid

    #235093

    Purpose

    Lbr mCherry fusion protein with MCP domain

    Depositor

    Mitchell Guttman

    Article

    Bhat et al Nature. 2024 May;629(8014):1165-1173. do

    Insert

    pCAG-MCP-Lbr-V5-mCherry (LBR Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Tbg-CES2C-ΔC-S230A

    Plasmid

    #200561

    Purpose

    Secreted mouse CES2C S230A mutant driven by heptaocyte-specific promoter in AAV viral vector

    Depositor

    Jonathan Long

    Article

    Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

    Insert

    Mouse Carboxylesterase 2C S230A mutant delta C-terminal (Ces2c Mouse)
    

    Use

    AAV

    Tags

    Flag

    Promoter

    TBG promoter

    Available Since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2-CIB1-mRuby-Rab35

    Plasmid

    #194334

    Purpose

    For use in light-induced Rab35 protein inactivation through the interaction with CRY2

    Depositor

    Heidi Hehnly

    Article

    Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

    Inserts

    CIB1 (CIB1 Mustard Weed)
    Rab35 (RAB35 Human)
    

    Tags

    mRuby

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. AICSDP-15:ACTB-mEGFP

    Plasmid

    #87425

    Purpose

    Homology arms and linker-mEGFP sequence for N-terminus tagging of human ACTB

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    ACTB Homology Arms with mEGFP-linker (ACTB Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    March 21, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pCAG-MCP-dNS-SRRM1-V5-mCherry

    Plasmid

    #235095

    Purpose

    SRRM1 lacking Nuclear Speckle domain (dNS-SRRM1) mCherry fusion protein with MCP domain

    Depositor

    Mitchell Guttman

    Article

    Bhat et al Nature. 2024 May;629(8014):1165-1173. do

    Insert

    pCAG-MCP-dNS-SRRM1-V5-mCherry (SRRM1 Human)
    

    Expression

    Mammalian

    Mutation

    The SRRM1 entry clone from DNASU was modified usi…

    Promoter

    CAG

    Available Since

    May 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-SpyTag-Flag-dNS-SRRM1-V5-mCherry

    Plasmid

    #235096

    Purpose

    SRRM1 lacking Nuclear Speckle domain (dNS-SRRM1) mCherry fusion protein without MCP domain

    Depositor

    Mitchell Guttman

    Article

    Bhat et al Nature. 2024 May;629(8014):1165-1173. do

    Insert

    pCAG-SpyTag-Flag-dNS-SRRM1-V5-mCherry (SRRM1 Human)
    

    Expression

    Mammalian

    Mutation

    The SRRM1 entry clone from DNASU was modified usi…

    Promoter

    CAG

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV_msfGFP-SEPT7

    Plasmid

    #180315

    Purpose

    mammalian expression of human SEPT7 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Insert

    SEPTIN7 (SEPTIN7 Human)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV_SEPT2-msfGFP

    Plasmid

    #180318

    Purpose

    mammalian expression of human SEPT2 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Insert

    SEPTIN2 (SEPTIN2 Human)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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