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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,126 results for: LEA

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Showing: 9981 - 10000 of 12126 results

  5. pET21b-hHNF4aORF-1

    Plasmid
    #206959
    Purpose
    The ORF of HNF4α (variant 2) cDNA amplified from the RT human liver RNA was cloned at the NheI/NotI sites of the pET21b(+), the bacterial expression vector.
    Depositor
    Catharine Ross, Reza Zolfaghari
    Insert
    HNF4a (Hepatocyte Nuclear Factor alpha4 Variant 2) (HNF4A Human)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    206959-plasmid-map-sequence-id-422372

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  6. pETM11-6xHis-TEV-hORF1p_StammerAAA

    Plasmid
    #202587
    Purpose
    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A, E92A, and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    ORF1p
    Tags
    6x His and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…
    Available Since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    202587-plasmid-map-sequence-id-415633

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  10. pRegev_S1.34C>A_46G>U

    Plasmid
    #204161
    Purpose
    pRegev_S1 with double mutations that restore secondary structure.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    S1 exon with 34C>A and 46G>U substitutions in beta globin splicing minigene
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    34C>A and 46G>U mutations
    Promoter
    Truncated CAG
    Available Since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204161-plasmid-map-sequence-id-405452

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  11. pRegev_S2.39U>G.51A>C

    Plasmid
    #204165
    Purpose
    pRegev_S2 with double mutations that restore secondary structure.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    Exon S2 with 39U>G and 51A>C mutations in beta globin splicing minigene
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    39U>G and 51A>C mutations
    Promoter
    Truncated CAG
    Available Since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204165-plasmid-map-sequence-id-405443

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  12. pRegev_S5.49C>A.59G>U

    Plasmid
    #204177
    Purpose
    pRegev_S5 with double mutations that restore secondary structure.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    Exon S5 with 49C>A and 59G>U mutations in beta globin splicing minigene
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    49C>A and 59G>U mutations
    Promoter
    Truncated CAG
    Available Since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204177-plasmid-map-sequence-id-404686

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  13. pENeCFPCNAL2 pc922

    Plasmid
    #167563
    Purpose
    For construction of CFP-tagged human PCNA, the GFP coding sequence in the pENeGFPCNAL2 vector was replaced by the ECFP coding sequence from the pECFP-C1 vector (Clontech Laboratories, Inc., CA).
    Depositor
    Cristina Cardoso
    Article
    Zhang et al Nucleic Acids Res. 2017 Apr 25. doi: 10.
    Inserts
    Proliferating Cell Nuclear Antigen (PCNA Human)
    CFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    167563-plasmid-map-sequence-id-403652

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  17. pCAGGS-C-TEV-HAT

    Plasmid
    #202597
    Purpose
    Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Type
    Empty backbone
    Tags
    TEV cleavable site (ENLYFQG) and poly-histidine a…
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202597-plasmid-map-sequence-id-399552

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Showing: 9981 - 10000 of 12126 results