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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 35,752 results for: lat

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Showing: 9981 - 10000 of 35752 results

  4. pCC_06 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139091
    Purpose
    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-NG-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139091-plasmid-map-sequence-id-273888

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  7. EBA140-bio

    Plasmid
    #47742
    Purpose
    Expresses enzymatically monobiotinylated full-length EBA140 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised EBA140
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47742-plasmid-map-sequence-id-68750

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  8. PTEX150-bio

    Plasmid
    #47788
    Purpose
    Expresses enzymatically monobiotinylated full-length PTEX150 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised PTEX150
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47788-plasmid-map-sequence-id-68825

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  10. hNTo2-qgRNA-pYJA5

    Plasmid
    #217782
    Purpose
    Non-targeting control 2 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Adriano Aguzzi
    Article
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene ablation
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    217782-plasmid-map-sequence-id-466020

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  13. XLone-Puro beta-catenin P2A eGFP-NLS

    Plasmid
    #155189
    Purpose
    PiggybacTransposon-based tunable and temporal expression control of mutated beta-catenin and nuclear eGFP
    Depositor
    Xiaoping Bao
    Article
    Chang et al Biomaterials. 2022 May 6;285:121569. doi
    Insert
    beta-catenin (Ctnnb1 Mouse)
    Use
    Unspecified; Piggybac transposon
    Tags
    P2A eGFP NLS
    Expression
    Mammalian
    Mutation
    Sites phosphorylated by GSK-3beta have been mutat…
    Promoter
    TRE3G
    Available Since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    155189-plasmid-map-sequence-id-307963

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  14. pEN527 - Rad21-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct

    Plasmid
    #156452
    Purpose
    Targeting vector to introduce an AID-eGFP cassette at the mouse RAD21 (SCC1) locus using BLASTICIDIN selection. Auxin-inducible degron system. Designed to be used with sgRNA CCACGGTTCCATATTATCTG
    Depositor
    Elphege Nora
    Article
    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:
    Insert
    RAD21, AID (Rad21 Mouse)
    Use
    Mouse Targeting
    Expression
    Mammalian
    Mutation
    none
    Available Since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    156452-plasmid-map-sequence-id-310171

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  17. HA-hDAGLa-V5

    Plasmid
    #87674
    Purpose
    Expresses human DAGLa protein with a HA tag inserted in the first extracellular loop, and intracellular V5 tag located on the C-terminal
    Depositor
    Pat Doherty
    Article
    Zhou et al Mol Cell Neurosci. 2016 Oct;76:76-86. do
    Insert
    Human Diacylglycerol Lipase Alpha (DAGLA Human)
    Tags
    HA and V5 tag
    Expression
    Mammalian
    Mutation
    Hemagglutinin (HA) peptide sequence (YPYDVPDYA) f…
    Promoter
    T7
    Available Since
    March 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    87674-plasmid-map-sequence-id-173603

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  18. GFP-DroshaS300E/S302D

    Plasmid
    #62531
    Purpose
    Serine 300 of Drosha is mutated to glutamic acid and serine 302 is mutated to aspartic acid
    Depositor
    Bharat Ramratnam
    Article
    Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-
    Insert
    human Drosha with serine 300 changed to glutamic acid and serine 302 changed to aspartic acid (DROSHA Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    changed serine 300 to glutamic acid and serine 3…
    Available Since
    March 20, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 9981 - 10000 of 35752 results