165,839 results

1. pZH81 pET21b Rat1-His

   Plasmid

   #231650

   Purpose

   Bacterial expression vector expressing yeast S. cerevisiae rat1 with a C-terminal his tag

   Depositor

   Jesper Svejstrup

   Article

   Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

   Insert

   Rat1 (RAT1 Budding Yeast)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZH82 pET28a-Rai1

   Plasmid

   #231651

   Purpose

   Bacterial expression vector expressing yeast S. cerevisiae rai1, no tag

   Depositor

   Jesper Svejstrup

   Article

   Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

   Insert

   rai1 (RAI1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZH23 Dual G-less containing ySSA1 polyA

   Plasmid

   #231647

   Purpose

   yeast S.cerevisiae SSA1 polyA termination region inserted between the dual G-less cassette

   Depositor

   Jesper Svejstrup

   Article

   Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

   Insert

   SSA1 polyA termination region
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZH20 Dual G-less containing yCYC1 polyA

   Plasmid

   #231646

   Purpose

   yeast S.cerevisiae CYC1 polyA termination region inserted between the dual G-less cassette

   Depositor

   Jesper Svejstrup

   Article

   Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

   Insert

   CYC1 polyA termination region
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. 4XCLEAR-luciferase reporter

   Plasmid

   #66800

   Purpose

   Reporter for activity of the CLEAR network

   Depositor

   Albert La Spada

   Article

   Cortes et al Nat Neurosci. 2014 Sep;17(9):1180-9. doi

   Insert

   4XCLEAR
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Four CLEAR elements (GTCACGTGAC) in tandem derive…

   Available Since

   Nov. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5-HA-Ubiquitin-K33

   Plasmid

   #17607

   Purpose

   Mammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to arginines

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K33 only, other lysines mutated to arginines; P19…

   Promoter

   Available Since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFlp-Km

   Plasmid

   #222334

   Purpose

   Expresses Flp and kanamycin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-neo-pBBR1-ELT3

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Inserts

   sacB (sacB B. subtilis)
   flp (flp Budding Yeast)
   cI (cI )
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   cl, flp, and sacB

   Available Since

   Aug. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBHRSF184

   Plasmid

   #89482

   Purpose

   Express asparaginyl endopeptidase 1 from O. affinis (OaAEP1)

   Depositor

   Hideo Iwai

   Article

   Mikula et al FEBS Lett. 2017 Mar 30. doi: 10.1002/187

   Insert

   Asparaginyl endopeptidase from Oldenlandia affinis
   

   Use

   Tags

   Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tet-O-FUW-ZEB2-P2A-mCherry

   Plasmid

   #203857

   Purpose

   Tet-inducible lentiviral plasmid expressing ZEB2-P2A-mCherry, with ORF specific barcode close to 3'LTR site

   Depositor

   Andrea Califano

   Article

   Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

   Insert

   ZEB2 (ZEB2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-All-in-one-hCascade

    Plasmid

    #134919

    Purpose

    Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cascade factors (Cse1(Cas8), Cse2(Cas11), Cas5, Cas6, Cas7) with bpNLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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