We narrowed to 106,694 results for: cell;

1. pSJ2039 (pRS316-NOP1pr-GFP1-10-SCS2TM)

   Plasmid

   #86418

   Purpose

   ONM/ER split GFP1-10

   Depositor

   Sue Jaspersen

   Article

   Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

   Insert

   NOP1pr-GFP1-10-Scs2TM
   

   Tags

   GFP1-10

   Expression

   Yeast

   Promoter

   NOP1

   Available Since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pG2J

   Plasmid

   #102882

   Purpose

   Expresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.

   Depositor

   Matthias Heinemann

   Article

   Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

   Inserts

   OsTIR1
   mGFP-AID
   

   Tags

   AID(71-114)

   Expression

   Yeast

   Promoter

   ADH1 and TEF1

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Flag p21 T145A

   Plasmid

   #16241

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2001 Mar . 3(3):245-52.

   Insert

   p21 (CDKN1A Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   T145A

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

4. 5HT6-YFP-Inp54p(D281A)

   Plasmid

   #96813

   Purpose

   phosphatase-dead control for 5HT6-YFP-Inp54p

   Depositor

   Takanari Inoue

   Article

   Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

   Insert

   5HT6-YFP-Inp54p(D281A)
   

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   D281A inactivates phosphatase activity

   Available Since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Flag p21 T145D

   Plasmid

   #16242

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2001 Mar . 3(3):245-52.

   Insert

   p21 (CDKN1A Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   T145D

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

6. ML1Nmut_in_pEGFP-C1

   Plasmid

   #67796

   Purpose

   Mammalian expression vector driving Mouse ML1Nwt (2xML1N domain 7Q mutant version) fused to EGFP

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   ML1N*2(7Q)-EGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRcCMV myc-Plk1 wt (Nigg RG6)

   Plasmid

   #41160

   Depositor

   Erich Nigg

   Article

   Golsteyn et al J Cell Sci. 1994 Jun;107 ( Pt 6):1509-17

   Insert

   Plk1 (PLK1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroR

   Plasmid

   #162334

   Purpose

   Lentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistance

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   dSaCas9-KRAB
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   hUbC

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-Rab21-CCSS

   Plasmid

   #83424

   Purpose

   Mouse Rab21, mutation of the putative COOH-terminal prenylation motif (residues 218 and 219 from C to S). Generated from pEGFP-Rab21 by site-directed mutagenesis.

   Depositor

   Johanna Ivaska

   Article

   Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

   Insert

   Rab21 (Rab21 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Residues 218 and 219 from C to S (CCSS: TGCTGT -&…

   Promoter

   CMV

   Available Since

   Nov. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR-JNKKTRmRuby2

    Plasmid

    #59148

    Purpose

    ORF encoding JNK KTR mRuby2 flanked by Gateway sequences

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    JNK Kinase Translocation Reporter (MAPK8 Human, Mouse)
    

    Tags

    mRuby2

    Available Since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. Bmi1 shRNA1

    Plasmid

    #21576

    Depositor

    Sally Temple

    Article

    Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

    Insert

    Bmi-1 (Bmi1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. ETS1_pet28a

    Plasmid

    #131663

    Purpose

    Bacterial expression of ETS gene ETS1

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ETS1 (ETS1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR-PPP2R2B

    Plasmid

    #16181

    Depositor

    Huda Zoghbi

    Article

    Lim et al Cell. 2006 May 19. 125(4):801-14.

    Insert

    PP2A regulatory subunit B, beta isoform (PPP2R2B Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pLUT7 HA-GLI1

    Plasmid

    #62970

    Purpose

    Expresses HA-tagged Gli1

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    GLI1 (GLI1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK5 DN-Fyn

    Plasmid

    #16033

    Depositor

    Filippo Giancotti

    Article

    Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

    Insert

    c-Fyn (FYN Human)
    

    Expression

    Mammalian

    Mutation

    Dominant negative: K299M

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

16. CAG-DDdCas9VP192-T2A-EGFP-ires-puro

    Plasmid

    #69534

    Purpose

    DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

    Insert

    DD-dCas9VP192-T2A-EGFP
    

    Tags

    C-term T2A-EGFP and DHFR Destabilised Domain

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pC-(loxP2-attB2-SA(2)-T2A-Gal4-Hsp70)

    Plasmid

    #62956

    Purpose

    Singlet donor (phase 2) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4-Hsp70
    

    Available Since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. CAG-Cremyc-2A-GFP

    Plasmid

    #116879

    Purpose

    Express Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.

    Depositor

    Qiang Lu

    Article

    Geng et al Nat Commun. 2018 Jul 13;9(1):2707. doi:

    Insert

    Cremyc-2A-GFP
    

    Expression

    Mammalian

    Available Since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. 5HT6-YFP-PIPK(D253A)

    Plasmid

    #96811

    Purpose

    kinase-dead control for 5HT6-YFP-PIPK

    Depositor

    Takanari Inoue

    Article

    Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

    Insert

    5HT6-YFP-PIPK(D253A)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    D253A inactivates kinase activity of PIPK

    Available Since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. ETV4_pet28a

    Plasmid

    #131645

    Purpose

    Bacterial expression of ETS gene ETV4

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ETV4 (ETV4 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Oct. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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