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pAV-U6+27-tCorn

Plasmid

#106233

Purpose

Express tRNA-Corn as a reporter of U6 promoter activity in mammalian cells

Depositor

Samie Jaffrey

Article

Song et al Nat Chem Biol. 2017 Nov;13(11):1187-1194

Insert

tRNA-Corn

Expression

Mammalian

Promoter

U6

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pKH116_keima-YIPF3

Plasmid

#214970

Purpose

Golgiphagy flux reporter keima-YIPF3

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

keima-YIPF3 (YIPF3 Human)

Use

Lentiviral

Available Since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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pPUR-mU6-sgRNA-Sirius-8XPP7

Plasmid

#121943

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-8XPP7

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

mouse U6 promoter

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Tubulin-hfYFP

Plasmid

#186515

Purpose

Localization of hyperfolder YFP fluorescent protein to tubulin

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Hyperfolder YFP

Tags

Tubulin

Expression

Mammalian

Mutation

mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-H-Luc_cyto

Plasmid

#113929

Purpose

Plasmid encoding the cytosolic localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells for BRET

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

H-Luc

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pUBC-SiT-EGFP-MS2

Plasmid

#247294

Purpose

Expresses a secretome mRNA reporter: MS2 mRNA encoding 45aa of the Type II membrane protein, sialyltransferase (a Golgi enzyme), fused with EGFP.

Depositor

Jennifer Lippincott-Schwartz

Article

Choi et al Nature. 2025 Nov 5. doi: 10.1038/s41586-

Insert

SiT-EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

UbC

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-hfYFP

Plasmid

#191201

Purpose

Constitutive CAG-driven expression of hfYFP in animals using AAV

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Hyperfolder YFP

Use

AAV

Expression

Mammalian

Mutation

mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

Promoter

CAG

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pTC217

Plasmid

#70018

Purpose

Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b

Depositor

Daniel Voytas

Article

Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

Insert

Nuclease (Cas9/sgRNA) + Donor + GVR

Use

CRISPR

Expression

Plant

Available Since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

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PCMV-intron myc Rab2WT

Plasmid

#46779

Purpose

expression of WT Rab2a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

RAB2 (RAB2A Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pGEX6P1-hPREP

Plasmid

#179387

Purpose

Express human prolyl oligopeptidase (tagged) in bacterial cells

Depositor

Christian Gruber

Article

Retzl et al J Nat Prod. 2020 Nov 25;83(11):3305-3314

Insert

human prolyl oligopeptidase (PREP Human)

Tags

GST

Expression

Bacterial

Promoter

TAC

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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