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TH1150-pOCC177-FUS(Nhe)-PLD_YtoF_RGG_RtoK

Plasmid

#221889

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

FUS (FUS Human)

Tags

6xHis-MBP-PreScission and TEV-SNAP-tag

Expression

Insect

Mutation

In PLD all Tyr (Y) changed to Phe (F), in RGG (aa…

Promoter

PH promoter

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST (E44A L47A D49A A50K Q51A; deltaWIPI2)

Plasmid

#223777

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

BNIP3 soluble part (BNIP3 Human)

Tags

Thrombin cleavage-GST

Expression

Insect

Mutation

E44A L47A D49A A50K Q51A; disrupts WIPI2 binding

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pBIG1a_StrepII-Hook2_FTS_FHIP2A

Plasmid

#222302

Purpose

Co-expresses full-length human FHF (FTS, StrepII-HOOK2 and FHIP2A) in a pBIG1a vector

Depositor

Andrew Carter

Article

Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

Inserts

HOOK2 (HOOK2 Human)
FTS (AKTIP Human)
FHIP2A (FHIP2A Human)

Tags

StrepII-Psc

Expression

Insect

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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TH1274-054_Jie_EWSR1_pOCC177_pOEM1-N-HIS-MBP-PS-NotI-AscI-TEV-SNAP

Plasmid

#221896

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

EWSR1 (EWSR1 Human)

Tags

6xHis-MBP-PreScission and TEV-SNAP-tag

Expression

Insect

Mutation

wt

Promoter

PH promoter

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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TH0994-pOCC177-Fus-wt_(1-211)

Plasmid

#221886

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

FUS (FUS Human)

Tags

6xHis-MBP-PreScission and TEV-SNAP-tag

Expression

Insect

Mutation

PLD domain (aa 1-211)

Promoter

PH promoter

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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TH1148-pOCC177-FUS(Nhe)-PLD_YtoF

Plasmid

#221887

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

FUS (FUS Human)

Tags

6xHis-MBP-PreScission and TEV-SNAP-tag

Expression

Insect

Mutation

In PLD all Tyr (Y) changed to Phe (F)

Promoter

PH promoter

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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TH1149-pOCC177-FUS(Nhe)-RGG_RtoK

Plasmid

#221888

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

FUS (FUS Human)

Tags

6xHis-MBP-PreScission and TEV-SNAP-tag

Expression

Insect

Mutation

In RGG (aa 212-526) most of Arg (R) changed to Ly…

Promoter

PH promoter

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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TH1202-pOCC175_pOEM1-N-HIS-MBP-PS-mGFP-TEV-HNRNPA3_opt

Plasmid

#221890

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

HNRNPA3 (HNRNPA3 Human)

Tags

6xHis-MBP-PreScission-mGFP-TEV

Expression

Insect

Mutation

wt

Promoter

PH promoter

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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FUW-hSNCA(D2R)-NE

Plasmid

#215487

Purpose

Lentiviral expression of mutated alpha-synuclein (2nd amino acid aspartate mutated to arginine) in mammalian cells

Depositor

Ophir Shalem

Article

Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

Insert

alpha-synuclein (SNCA Human)

Use

Lentiviral

Tags

NE tag

Expression

Mammalian

Mutation

D (second aa) mutated to R

Promoter

Ubc

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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FUW-hSNCA(D2E)-NE

Plasmid

#215488

Purpose

Lentiviral expression of mutated alpha-synuclein (2nd amino acid aspartate mutated to glutamate) in mammalian cells

Depositor

Ophir Shalem

Article

Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

Insert

alpha-synuclein (SNCA Human)

Use

Lentiviral

Tags

NE tag

Expression

Mammalian

Mutation

D (second aa) mutated to E

Promoter

Ubc

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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FUW-hSNCA(D2R)-GFP

Plasmid

#215490

Purpose

Lentiviral expression and easy visualization of mutated alpha-synuclein (2nd amino acid aspartate mutated to arginine) in mammalian cells

Depositor

Ophir Shalem

Article

Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

Insert

alpha-synuclein (SNCA Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

D (second aa) mutated to R

Promoter

Ubc

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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FUW-hSNCA(D2E)-GFP

Plasmid

#215491

Purpose

Lentiviral expression and easy visualization of mutated alpha-synuclein (2nd amino acid aspartate mutated to glutamate) in mammalian cells

Depositor

Ophir Shalem

Article

Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

Insert

alpha-synuclein (SNCA Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

D (second aa) mutated to E

Promoter

Ubc

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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SHIP2-C2

Plasmid

#214906

Purpose

expression of the truncated variants of the SHIP2 that retains second proline-rich domain

Depositor

Pavel Krejčí

Article

Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608

Insert

human SHIP2 aa 740-1189 (INPPL1 Human)

Tags

V5/His

Expression

Mammalian

Mutation

delta 1-739, 1190-1258aa

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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SHIP2-C1

Plasmid

#214905

Purpose

expression of the truncated variants of the SHIP2 that retains second proline-rich domain and C-terminal sterile alpha motif

Depositor

Pavel Krejčí

Article

Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608

Insert

human SHIP2 aa 740-1258 (INPPL1 Human)

Tags

V5/His

Expression

Mammalian

Mutation

delta 1-739aa

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pUb FLAG-Mts D85N

Plasmid

#208403

Purpose

Expresses FLAG-tagged Drosophila mts protein with D85N mutation

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

microtubule star (mts Fly)

Tags

3xFLAG

Expression

Insect

Mutation

D85N

Promoter

Ubi-p63e

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pUb FLAG-Mts H59Q

Plasmid

#208402

Purpose

Expresses FLAG-tagged Drosophila mts protein with H59Q mutation

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

microtubule star (mts Fly)

Tags

3xFLAG

Expression

Insect

Mutation

H59Q

Promoter

Ubi-p63e

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pUb FLAG-Mts H241A

Plasmid

#208404

Purpose

Expresses FLAG-tagged Drosophila mts protein with H241A mutation

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

microtubule star (mts Fly)

Tags

3xFLAG

Expression

Insect

Mutation

H241A

Promoter

Ubi-p63e

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-FAS-COMP5AP-AviTag-9xHis

Plasmid

#157106

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

FAS (FAS Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-DSCAML1(Trunc1)-COMP5AP-AviTag-9xHis

Plasmid

#157607

Purpose

Mammalian expression of cell-surface protein partial extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

DSCAML1 (DSCAML1 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-DSCAM(Trunc1)-COMP5AP-AviTag-9xHis

Plasmid

#157608

Purpose

Mammalian expression of cell-surface protein partial extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

DSCAM (DSCAM Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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