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pDONR221_MFSD14A

Plasmid

#131869

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

MFSD14A (HIAT1 Human)

Expression

Mammalian

Available Since

Oct. 9, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-CLN3_STOP

Plasmid

#161034

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

CLN3 (CLN3 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC3A2_STOP

Plasmid

#161379

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC3A2 (SLC3A2 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Neurog2 x2)

Plasmid

#171100

Purpose

CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Neurog2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9 (Neurog2 Mouse, S. pyogenes)

Use

CRISPR

Promoter

Ef1a

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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LgBiT-hACE2

Plasmid

#173431

Purpose

protein expression plasmid of LgBiT-hACE2-IgG1 Fc

Depositor

David Nemazee

Article

Huang et al J Immunol. 2021 Jul 1;207(1):344-351. do

Insert

LgBiT-hACE2-IgG1 Fc (ACE2 Human)

Use

Luciferase

Tags

IgG1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC25A13

Plasmid

#132038

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A13 (SLC25A13 Human)

Expression

Mammalian

Available Since

Nov. 1, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-MFSD14A_STOP

Plasmid

#161035

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

MFSD14A (HIAT1 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC1A5_STOP

Plasmid

#161140

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC1A5 (SLC1A5 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-EF1a-CD90.2-Rluc_miR

Plasmid

#163360

Purpose

Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase, with expression of a CD90.2 surface marker.

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Insert

CD90.2 (Thy1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

EF1

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-SRPK1

Plasmid

#23582

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SRPK1 (SRPK1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR221_CLN3

Plasmid

#131868

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

CLN3 (CLN3 Human)

Expression

Mammalian

Available Since

Oct. 9, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223-NME2

Plasmid

#23392

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NME2 (NME2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-WNK1

Plasmid

#23670

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

WNK1 (WNK1 Human)

Use

Gateway donor vector

Mutation

G4C

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PAK4

Plasmid

#23713

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PAK4 (PAK4 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-TRIB3

Plasmid

#23677

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TRIB3 (TRIB3 Human)

Use

Gateway donor vector

Mutation

P5T

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PRKCB2

Plasmid

#23764

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKCB2 (PRKCB Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-PRKAR1B

Plasmid

#23376

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKAR1B (PRKAR1B Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
P932_CLYBL-CBX3-Cbh-Zeo-TetO-NEUROG2

Plasmid

#202762

Purpose

Encodes one forebrain neuron specific transcription factor under the control of a TetOn promoter at the CLYBL safe harbor

Depositor

Karl Wahlin

Article

Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

Insert

NEUROG2 (NEUROG2 Human)

Expression

Mammalian

Promoter

TetO

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 N-term FBL

Plasmid

#187577

Purpose

Express FLAG epitope and APEX2-tagged FBL fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

FBL (FBL Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-SNAP-hCB2_IRES_EGFP

Plasmid

#223506

Purpose

Expression of CB2 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection marker

Depositor

James Frank

Article

Kosar et al J Am Chem Soc. 2023 Jul 19;145(28):15094

Insert

SNAP-tag-CNR2 and EGFP (CNR2 Synthetic, Human)

Tags

SNAP-tag

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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