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p2xFlag CMV2-YAP2-2nd WW mutant

Plasmid

#19047

Depositor

Marius Sudol

Publication

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Tryptophan 258 changed to Alanine, and Proline 2…

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
integrin beta6 pcDNA1 neo

Plasmid

#13580

Depositor

Dean Sheppard

Publication

Weinacker et al J Biol Chem. 1994 Mar 4. 269(9):6940-8.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

integrin beta 6 (ITGB6 Human)

Expression

Mammalian

Available since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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MyoD-pCLBabe

Plasmid

#20917

Depositor

Stephen Tapscott

Publication

Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

Insert

MyoD (Myod1 Mouse)

Use

Retroviral

Expression

Mammalian

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
pAK405

Plasmid

#37114

Depositor

Julia Vorholt

Publication

Kaczmarczyk et al Appl Environ Microbiol. 2012 May;78(10):

Type

Empty backbone

Expression

Bacterial

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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HRV-B3 IRES

Plasmid

#189969

Purpose

Part 2 plasmid to introduce the HRV-B3 IRES into circRNAs

Depositor

Howard Chang

Publication

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

HRV-B3 IRES

Use

Cloning

Promoter

N/A

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTEK-4S-0X

Plasmid

#17974

Depositor

Sabine Ehrt

Publication

Guo et al J Bacteriol. 2007 Jul . 189(13):4614-23.

Insert

tetR(B)r1.7

Use

Mycobacteria expression

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only

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PGKneolox2DTA

Plasmid

#13443

Depositor

Philippe Soriano

Publication

Soriano et al Development. 1997 Jul . 124(14):2691-700

Insert

PGKneolox2DTA

Use

Cre/Lox

Expression

Mammalian

Available since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pJL1-mTagBFP2

Plasmid

#102638

Purpose

In vitro expression of mTagBFP2 from the T7 promoter

Depositor

Michael Jewett

Publication

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mTagBFP2

Expression

Bacterial

Promoter

T7

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pYPQ146

Plasmid

#69298

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 6 gRNAs

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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piggyBAC-H2B FR-MQV

Plasmid

#194079

Purpose

Expresses nuclear-localized FR-MQV in mammalian cells

Depositor

Ralph Jimenez

Publication

Mukherjee et al Biochemistry. 2020 Oct 6;59(39):3669-368

Insert

FR-MQV

Tags

Histone H2B

Expression

Mammalian

Mutation

Methionine 42 to Glutamine, Cystiene 159 to Valin…

Promoter

CMV7

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-BioID-FAM21

Plasmid

#121046

Purpose

Expresses GFP tagged BioID and human FAM21C in mammalian cells

Depositor

Gia Voeltz

Publication

Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

BioID
FAM21C (FAM21C Human)

Tags

GFP

Expression

Mammalian

Mutation

R118G (highly promiscuous form)

Promoter

CMV

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-W

Plasmid

#67975

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/ZsG markers

Depositor

Kosuke Yusa

Publication

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2AZsG cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pX462-hPRKAA1-gRNA_B

Plasmid

#74375

Purpose

gRNA_B to knockout human AMPK alpha 1 using Cas9n

Depositor

Reuben Shaw

Publication

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

human AMPK alpha 1 exon1 gRNA (PRKAA1 Human)

Use

CRISPR

Promoter

U6

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLVE-hTGFB3-IRES-Red

Plasmid

#52580

Purpose

2nd generation lentiviral transfer vector driving expression of human TGF-beta3 and RFP in mammalian cells

Depositor

Charles Gersbach

Publication

Brunger et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

Insert

human transforming growth factor-beta3 (TGFB3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1alpha

Available since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CAV1-K*R-mEGFP

Plasmid

#27766

Depositor

Ari Helenius

Publication

Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

caveolin-1, lysine null mutant (CAV1 Human)

Tags

monomeric EGFP

Expression

Mammalian

Mutation

All lysines mutated to arginines (K*R)

Available since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-NPY-ClopHensor

Plasmid

#25939

Depositor

Daniele Arosio

Publication

Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NPY-ClopHensor (NPY Aq. Victoria and Discosoma sp)

Tags

NPY and Strep-II

Expression

Mammalian

Mutation

Neuropeptide Y localization signal

Available since

Sept. 13, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFP C3-Lats1-PY1+2 mutant

Plasmid

#19054

Depositor

Marius Sudol

Publication

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Large tumor suppressor kinase 1, N mutant (LATS1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

PY1 mutant, (Tyrosine 376 changed to Alanine) and…

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pIB166

Plasmid

#90189

Purpose

E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter

Depositor

Indranil Biswas

Publication

Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

Type

Empty backbone

Tags

None

Expression

Bacterial

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GPR55-Tango

Plasmid

#66363

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Publication

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GPR55 (GPR55 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pDR123

Plasmid

#37149

Depositor

Dale Ramsden

Insert

XRCC5 (XRCC5 Human)

Expression

Insect

Available since

July 18, 2012

Availability

Academic Institutions and Nonprofits only