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pPB-CAG-shControl-mCherry-CAAX

Plasmid

#227689

Purpose

Membrane tethered mCherry and scramble of shRNA targeting Atg7

Depositor

Cagla Eroglu

Article

Wang et al eLife 2025 [Reviewed Preprint] 14:RP1075

Insert

scrambled shRNA control

Expression

Mammalian

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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Stx3-mCherry

Plasmid

#190890

Purpose

Expresses syntaxin 3 fused to mCherry in mammalian cells

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

Stx3 (Stx3 Rat)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-BbsI-Torso

Plasmid

#244933

Purpose

CRISPR gRNA targeting the C-terminal end of Torso for cleavage.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

gRNA targeting Torso C terminus

Use

CRISPR

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-BbsI-Btl

Plasmid

#244934

Purpose

CRISPR gRNA targeting the C-terminal end of Breathless for cleavage.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

gRNA targeting Btl C terminus

Use

CRISPR

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-BbsI-EGFR

Plasmid

#244932

Purpose

CRISPR gRNA targeting the C-terminal end of EGFR for cleavage.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

gRNA targeting EGFR C terminus

Use

CRISPR

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCK660

Plasmid

#242914

Purpose

Constitutive expression of dCas9, MCP-SoxS, and PspF-λN22

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

PspF-λN22 (pspF Synthetic)

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCK320

Plasmid

#242906

Purpose

aTc-inducible expression of dCas9-AsiA

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

AsiA (asiA Synthetic)

Use

CRISPR and Synthetic Biology

Promoter

TetR-Ptet

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1089

Plasmid

#242497

Purpose

Bacterial expression of GST-ScAtg38 Δ(123 to 226)

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

deletion of aa 123 to 226

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pOP768

Plasmid

#242496

Purpose

Bacterial expression of GST-ScAtg38 (87-226)

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

aa 87-226 only

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pYO780

Plasmid

#242481

Purpose

Expression of ScATG38 (FL)-2xEGFP under its own promoter

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

2xEGFP

Expression

Yeast

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1091

Plasmid

#242499

Purpose

Bacterial expression of GST-ScAtg38 Δ(123 to 181)

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

deletion of aa 123 to 181

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pYO785

Plasmid

#242488

Purpose

Expression of ScAtg38 (FL)-3xFLAG under its own promoter

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

3xFLAG

Expression

Yeast

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1093

Plasmid

#242501

Purpose

Bacterial expression of GST-ScAtg38 Δ(123 to 209)

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

deletion of aa 123 to 209

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pOP775

Plasmid

#242495

Purpose

Bacterial expression of GST-ScAtg38 (1-78)

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

aa 1-78 only

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1092

Plasmid

#242500

Purpose

Bacterial expression of GST-ScAtg38 Δ(182 to 209)

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

deletion of aa 182 to 209

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1090

Plasmid

#242498

Purpose

Bacterial expression of GST-ScAtg38 Δ(182 to 226)

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

deletion of aa 182 to 226

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1094

Plasmid

#242502

Purpose

Bacterial expression of GST-ScAtg38 Δ(210 to 226)

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

deletion of aa 210 to 226

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet_6xHis-TEV-mCherry

Plasmid

#227710

Purpose

Expression of recombinant protein for purification (used as control)

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

mCherry

Expression

Bacterial

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pEvol-chKlacRS-WT

Plasmid

#232159

Purpose

A chimeric PylRS for site-specific incorporation of L-lactyl-lysine into target proteins in E.coli cells.

Depositor

Nanxi Wang

Article

Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

Insert

chKlacRS-WT

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-EGFP-V5

Plasmid

#240240

Purpose

Expression of EGFP-V5 under control of copper-inducible promoter.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

EGFP-V5

Expression

Insect

Promoter

MT

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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