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Showing: 2001 - 2040 of 11184 results

  1. pBP-P_vanR-PVanCC

    Plasmid
    #204078
    Purpose
    Type 0 promoter part
    Depositor
    Mikhail Shapiro
    Insert
    vanR repressor and PVanCC vanillic acid-inducible promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204078-plasmid-map-sequence-id-413893

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  2. pTU2-a_Sv1KA_amilCP

    Plasmid
    #204054
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101-var1 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204054-plasmid-map-sequence-id-418176

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  3. pTU2-a_Sv2KA_amilCP

    Plasmid
    #204053
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101-var2 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204053-plasmid-map-sequence-id-418175

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  4. C79m

    Plasmid
    #120991
    Purpose
    MoClo golden gate assembly CD part for ccdA (Antitoxin to ccdB/C39m. Sequence taken from pMAR7 plasmid sequence). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ccdA antitoxin
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120991-plasmid-map-sequence-id-236074

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  5. V37m

    Plasmid
    #121043
    Purpose
    MoClo golden gate assembly AE destination vector DVCK-AE-mScar (ColE1/kan resistance destination vector with constitutive mScarlet expressing for screening.
    Depositor
    Richard Murray
    Insert
    Kan destination vector with ColE1 origin and mScarlet selection
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121043-plasmid-map-sequence-id-236355

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  6. C27m

    Plasmid
    #120965
    Purpose
    MoClo golden gate assembly CD part for TetR (tet repressor; identical to C2d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    tetR (no deg tags)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120965-plasmid-map-sequence-id-236387

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  7. C98m

    Plasmid
    #121002
    Purpose
    MoClo golden gate assembly CD part for Gp2 (RNA polymerase inhibitor from T7 phage; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Gp2 toxin
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121002-plasmid-map-sequence-id-236135

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  8. C51m

    Plasmid
    #120975
    Purpose
    MoClo golden gate assembly CD part for sfYFP (superfolder YFP; variant of https://www.fpbase.org/protein/yfp/ - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    sfYFP (has BbsI site)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120975-plasmid-map-sequence-id-236029

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  9. C33m

    Plasmid
    #120968
    Purpose
    MoClo golden gate assembly CD part for AiiA (AHL degradase; see doi: [10.1126/science.aaa3794]). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    AiiA degradase (Bennett, no ssra tag)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120968-plasmid-map-sequence-id-238044

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  10. pQdCas12a.empty

    Plasmid
    #236036
    Purpose
    The plasmid pQdCas12a.empty expresses the dCas12a endonuclease.
    Depositor
    Radhakrishnan Mahadevan
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    236036-plasmid-map-sequence-id-474357

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  11. pBP-P_nahR-PSalTTC

    Plasmid
    #204074
    Purpose
    Type 0 promoter part
    Depositor
    Mikhail Shapiro
    Insert
    nahR repressor and PSalTTC sodium salicylate-inducible promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    June 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    204074-plasmid-map-sequence-id-437326

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  12. pTU2-a_Sv5KA_amilCP

    Plasmid
    #204050
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101-var5 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    204050-plasmid-map-sequence-id-437162

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  13. pBP-T_BBa-B1006-U10

    Plasmid
    #204122
    Purpose
    Type 0 terminator part
    Depositor
    Mikhail Shapiro
    Insert
    BBa-B1006-U10 strong terminator
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204122-plasmid-map-sequence-id-418253

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  14. pTU2-a_Sv3KA_amilCP

    Plasmid
    #204052
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101-var3 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204052-plasmid-map-sequence-id-418172

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  15. pTU2-a_Sv6KA_amilCP

    Plasmid
    #204049
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101-var6 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204049-plasmid-map-sequence-id-418169

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  16. pTU2-a_Sv7KA_amilCP

    Plasmid
    #204048
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101-var7 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204048-plasmid-map-sequence-id-418164

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  17. pBP-P_luxR-PLuxB

    Plasmid
    #204073
    Purpose
    Type 0 promoter part
    Depositor
    Mikhail Shapiro
    Insert
    luxR-PLuxB repressor and PLuxB 3OC6-AHL-inducible promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204073-plasmid-map-sequence-id-413889

    Enlarge

  18. pTU2-a_Sv4KA_amilCP

    Plasmid
    #204051
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101-var4 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204051-plasmid-map-sequence-id-413880

    Enlarge

  19. T18m

    Plasmid
    #121037
    Purpose
    MoClo golden gate assembly DE part for L3S2P21 (382x-strength synthetic terminator with BsaI cut site removed; see DOI: 10.1038/nmeth.2515).
    Depositor
    Richard Murray
    Insert
    modified L3S2P21 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121037-plasmid-map-sequence-id-236351

    Enlarge

  20. V35m

    Plasmid
    #121042
    Purpose
    MoClo golden gate assembly AE destination vector DVCK-AE-GFP (ColE1/kan resistance destination vector with constitutive GFP expression for screening.
    Depositor
    Richard Murray
    Insert
    Kan destination vector with ColE1 origin and GFP selection
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121042-plasmid-map-sequence-id-247704

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  21. U17m

    Plasmid
    #121023
    Purpose
    MoClo golden gate assembly BC part for Bujard RBS (strong RBS from https://doi.org/10.1093/nar/25.6.1203). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Bujard RBS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121023-plasmid-map-sequence-id-245898

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  22. U19m

    Plasmid
    #121024
    Purpose
    MoClo golden gate assembly BC part for MG-aptamer (fluorescent RNA aptamer of malachite green; see DOI: 10.1021/ja0529788) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    MG-apt
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121024-plasmid-map-sequence-id-238066

    Enlarge

  23. C85m

    Plasmid
    #120996
    Purpose
    MoClo golden gate assembly CD part for sigmaX (Bacillus subtilis RNA polymerase involved in heat response and biofilm formation). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Sigma X (ECF20_992)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120996-plasmid-map-sequence-id-236125

    Enlarge

  24. C91m

    Plasmid
    #120999
    Purpose
    MoClo golden gate assembly CD part for sfCFP (superfolder CFP developed by Ye Chen in Matthew Bennet's lab) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    sfCFP (Chen, Bennett)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120999-plasmid-map-sequence-id-236129

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  25. C99m

    Plasmid
    #121003
    Purpose
    MoClo golden gate assembly CD part for codon-optimized mScarlet-I (fast-folding mScarlet variant, codon-optimized for E. coli; see https://www.fpbase.org/protein/mscarlet-i/).
    Depositor
    Richard Murray
    Insert
    mScarlet-I codon optimized
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121003-plasmid-map-sequence-id-236141

    Enlarge

  26. C21m

    Plasmid
    #120962
    Purpose
    MoClo golden gate assembly CD part for MG-aptamer (fluorescent RNA aptamer of malachite green; see DOI: 10.1021/ja0529788) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    MG-apt
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120962-plasmid-map-sequence-id-238043

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  27. p3m

    Plasmid
    #120935
    Purpose
    MoClo golden gate assembly AB part for J23150 (strength ~400x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    J23150
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120935-plasmid-map-sequence-id-235944

    Enlarge

  28. p4m

    Plasmid
    #120936
    Purpose
    MoClo golden gate assembly AB part for J23151 (strength ~900x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    J23151
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120936-plasmid-map-sequence-id-235945

    Enlarge

  29. pAAV-hSyn-GRAB_NTS1.0

    Plasmid
    #180006
    Purpose
    Expresses the neurotensin sensor GRAB_NTS1.0 in neurons
    Depositor
    Yulong Li
    Insert
    neurotensin sensor GRAB_NTS1.0
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available since
    April 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    180006-plasmid-map-sequence-id-358494

    Enlarge

  30. EcMscL

    Plasmid
    #92418
    Purpose
    E. coli Mechanosensitive Channel of Large Conductance
    Depositor
    Douglas Rees
    Insert
    MscL (mscL E. coli)
    Use
    Tags
    6x Histidine Tag and Thrombin Cleavage Site betwe…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    92418-plasmid-map-sequence-id-183401

    Enlarge

  31. C83m

    Plasmid
    #120994
    Purpose
    MoClo golden gate assembly CD part for pir (Initiation factor required for replication of R6K origin plasmids). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    pir protein required for R6K ori
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120994-plasmid-map-sequence-id-236103

    Enlarge

  32. U25m

    Plasmid
    #121027
    Purpose
    MoClo golden gate assembly BC part for RiboJ-UTR1 (very strong RBS, prefixed by self-cleaving RiboJ for upstream context independence). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    RiboJ + UTR1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121027-plasmid-map-sequence-id-238073

    Enlarge

  33. T16m

    Plasmid
    #121035
    Purpose
    MoClo golden gate assembly DE part for L3S2P55 (256x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    L3S2P55 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121035-plasmid-map-sequence-id-245903

    Enlarge

  34. T17m

    Plasmid
    #121036
    Purpose
    MoClo golden gate assembly DE part L3S2P11 (262x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    L3S2P11 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121036-plasmid-map-sequence-id-245428

    Enlarge

  35. pBP-P_TALEsp1_PUPsp1-phlF_PPhlF

    Plasmid
    #204080
    Purpose
    Type 0 promoter part
    Depositor
    Mikhail Shapiro
    Insert
    TALEsp1-stabilized promoter driving phlF repressor; PPhlF DAPG-inducible promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204080-plasmid-map-sequence-id-413895

    Enlarge

  36. pBP-P_lacI-Ptac-lacO

    Plasmid
    #204072
    Purpose
    Type 0 promoter part
    Depositor
    Mikhail Shapiro
    Insert
    lacI repressor and Ptac-lacO IPTG-inducible promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204072-plasmid-map-sequence-id-413888

    Enlarge

  37. U24m

    Plasmid
    #121026
    Purpose
    MoClo golden gate assembly BC part for RiboJ-BCD2 (low expression bi-cistronic RBS, engineered for context-independence, prefixed by self-cleaving RiboJ; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    RiboJ + BCD2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121026-plasmid-map-sequence-id-238071

    Enlarge

  38. T14m

    Plasmid
    #121033
    Purpose
    MoClo golden gate assembly DE part for L3S1P13 (178x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    L3S1P13 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121033-plasmid-map-sequence-id-236356

    Enlarge

  39. T15m

    Plasmid
    #121034
    Purpose
    MoClo golden gate assembly DE part for L3S3P11 (173x-strength synthetic terminator, modified to remove BsaI cut site; see DOI: 10.1038/nmeth.2515).
    Depositor
    Richard Murray
    Insert
    modified L3S3P11 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121034-plasmid-map-sequence-id-245426

    Enlarge

  40. C90m

    Plasmid
    #120998
    Purpose
    MoClo golden gate assembly CD part for NahR-sfGFP fusion (Adam Meyer improved NahR (C75m) fused to superfolder GFP (C3m)). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    NahRsfGFP fusion
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120998-plasmid-map-sequence-id-236128

    Enlarge

Showing: 2001 - 2040 of 11184 results