Addgene

We narrowed to 23,396 results for: CHI;

Showing: 1001 - 1020 of 23396 results

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His-tagged FL Rec8

Plasmid

#45243

Purpose

raise antibody against Rec8

Depositor

Michael Lampson

Article

Chiang et al Curr Biol. 2010 Sep 14;20(17):1522-8. do

Insert

Rec8 (Rec8 Mouse)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-XNS

Plasmid

#196655

Purpose

Promega's psiCHECK-2 Dual-Luciferase Reporter vector modified to allow the insertion of the target in opposite directions

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pSP64-membrane-mCherry-LOV

Plasmid

#135599

Purpose

Expression of membrane-mCherry-LOV mRNA for microinjection for use of optogenetics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

membrane-mCherry-LOV

Use

In vitro transcription (mrna synthesis)

Tags

LOV and mCherry

Expression

Mutation

Promoter

Unknown

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Actinin-sstFRET-GR

Plasmid

#83416

Purpose

FRET-based actinin tension sensor

Depositor

Tetsuya Kitaguchi

Article

Wang et al J Biomech Sci Eng (2016). Vol.11, No.4

Insert

Actinin-sstFRET-GR

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only
negActinin-sstFRET-GR

Plasmid

#83417

Purpose

FRET-based actinin tension sensor (negative control)

Depositor

Tetsuya Kitaguchi

Article

Wang et al J Biomech Sci Eng (2016). Vol.11, No.4

Insert

negActinin-sstFRET

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-CA

Plasmid

#69616

Purpose

To express gene strongly with AAV

Depositor

Naoshige Uchida

Article

Menegas et al Elife. 2015 Aug 31;4. doi: 10.7554/eLife

Type

Empty backbone

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-SpSuc1

Plasmid

#162558

Purpose

Expresses GST-tagged S.pombe Suc1 in E.coli

Depositor

Iain Hagan

Article

Singh et al Methods Mol Biol. 2021;2329:123-142. doi

Insert

Schizosaccharomyces pombe Suc1 (suc1 Fission Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGG>nls-hCas9-nls

Plasmid

#99138

Purpose

Expresses Cas9 via the chicken beta actin promoter for electroporation in chicken embryos

Depositor

Marianne Bronner

Article

Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

Insert

humanized Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Chicken beta actin (CAG)

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-6xHis-BglII

Plasmid

#196654

Purpose

Derived from pGLO (BioRad) by the insertion of a 6xHis Tag and a BglII site

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Type

Empty backbone

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pBT264

Plasmid

#27438

Depositor

Liqun Luo

Article

Miyamichi et al Nature. 2011 Apr 14;472(7342):191-6. doi

Insert

TRE-tdTomato-myc flanked by insulators

Use

Mouse Targeting; Tta dependent

Tags

Myc

Expression

Mutation

Promoter

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pBT140

Plasmid

#27493

Depositor

Liqun Luo

Article

Miyamichi et al Nature. 2011 Apr 14;472(7342):191-6. doi

Insert

Nuclear localization signal-Cre

Use

Tags

nuclear localization signal

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only

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pBSKΔB

Plasmid

#177268

Purpose

Backbone vector for STREAMING-tag knockin vector

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pWalium-ChtVis-Tomato

Plasmid

#67756

Purpose

Expresses a fluorescent chitin reporter under Gal4 control in Drosophila.

Depositor

Paul Adler

Article

Sobala et al Development. 2015 Nov 15;142(22):3974-81

Insert

dyl - chitin binding domain - tdTomato - dyl

Use

Tags

td-tomato

Expression

Insect

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2-H2B-mCherry

Plasmid

#135604

Purpose

Expression of H2B-mCherry mRNA for microinjection to visualize Histone 2B protein dynamics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

H2B-mCherry

Use

In vitro transcription (mrna synthesis)

Tags

mCherry

Expression

Mutation

Promoter

Unknown

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLR5-CAG-NLS-tdiRFP670

Plasmid

#82621

Purpose

Expresses NLS-tdiRFP670 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

Insert

NLS-tdiRFP670

Use

Piggybac vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pSP64-H2B-GFP

Plasmid

#135605

Purpose

Expression of H2B-GFP mRNA for microinjection to visualize Histone 2B protein dynamics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

H2B-GFP

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Expression

Mutation

Promoter

Unknown

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pBSKΔB-CBh-Cas9n-pA

Plasmid

#82617

Purpose

Expresses Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

Insert

SpCas9n

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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psiCHECK-EGFP

Plasmid

#196606

Purpose

Dual-Luciferase Reporter vector carrying an EGFP direct sequence

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pTS2401-Tier1-PhCMV-HygroR

Plasmid

#169493

Purpose

Tier-1 vector encoding PhCMV-driven hygromycin resistance gene HygroR (PhCMV-HygroR-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven hph

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSP64-EMTB-GFP-ePDZ

Plasmid

#135600

Purpose

Expression of EMTB-GFP-ePDZ mRNA for microinjection for use of optogenetics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

EMTB-GFP-ePDZ

Use

In vitro transcription (mrna synthesis)

Tags

EGFP and ePDZ

Expression

Mutation

Promoter

Unknown

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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