We narrowed to 32,009 results for: Eng

1. CMV_Npu-BE3.9max C-terminal

   Plasmid

   #137172

   Purpose

   Expresses the C-terminal split-intein fragment of BE3.9max from the CMV promoter

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   Npu-BE3.9max_C-terminal
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. amilGFP chromoprotein promoter-less

   Plasmid

   #118006

   Purpose

   For course in "Synthetic Biology: A Lab Manual"

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   RBS, amilGFP
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMV_Npu-ABEmax C-terminal

   Plasmid

   #137174

   Purpose

   Expresses the C-terminal split-intein fragment of ABEmax from the CMV promoter

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   Npu-ABEmax_C-terminal
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPPC011

   Plasmid

   #171143

   Purpose

   Expression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Insert

   Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   SoxS has R93A and S101A mutations

   Promoter

   Sp.pCas9, BBa_J23107

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. tsPurple chromoprotein promoter-less

   Plasmid

   #118007

   Purpose

   For course in "Synthetic Biology: A Lab Manual"

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   RBS, tsPurple
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. ku70 insUP+pPGI+NAT+tNOS+ku70 insD (SBE217)

   Plasmid

   #195049

   Purpose

   integration cassette for ku70 deletion and expression of NAT gene under pPGI for characterization

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   NAT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pPGI

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. promoter J23110 (upstream of RBS, mRFP1)

   Plasmid

   #117852

   Purpose

   For course in "Synthetic Biology: A Lab Manual"

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   medium-high promoter, RBS, mRFP1
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPPC001

   Plasmid

   #171138

   Purpose

   For integration of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Insert

   Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   SoxS has R93A and S101A mutations

   Promoter

   Sp.pCas9, BBa_J23107

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. H446 EF1a RA-ABE6.3 p2A Puro

   Plasmid

   #139104

   Purpose

   Lentiviral expression of ABE6.3.

   Depositor

   Wen Xue

   Article

   Song et al Nat Biomed Eng. 2019 Feb 25. pii: 10.103

   Insert

   ABE RA6.3
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. (K) tNOS (S)_T3 (SBE120)

    Plasmid

    #195010

    Purpose

    terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T3 position (K/S)

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (K) tNOS (S)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. (H) tNOS (I) _T2 (SBE119)

    Plasmid

    #195006

    Purpose

    terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T2 position (H/I)

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (H) tNOS (I)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. (B) HYG marker(R) (SBE264)

    Plasmid

    #195013

    Purpose

    hygromycin (HYG) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    pXYL+HYG+tGPD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pXYL

    Available since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. (E) tNOS (F) _T1 (SBE118)

    Plasmid

    #194997

    Purpose

    terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T1 position (E/F)

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (E) tNOS (F)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. (B) BLE marker(R) (SBE262)

    Plasmid

    #195012

    Purpose

    bleomycin (BLE) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    pXYL+BLE+tGPD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pXYL

    Available since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZ_lacO-cP-AOX1-eGFP

    Plasmid

    #126720

    Purpose

    yeast plasmid overexpressing eGFP

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    egfp
    

    Use

    Tags

    6xHis,Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. p1585588

    Plasmid

    #62620

    Purpose

    ePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLAR (1:3:3 Ratio)

    Depositor

    Mattheos Koffas

    Article

    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

    Inserts

    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    dihydroflavonol 4-reductase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    leucoanthocyanidin reductase
    

    Use

    Synthetic Biology

    Tags

    GBD, PDZ, and SH3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCHC025 (∆CBBp)

    Plasmid

    #226203

    Purpose

    Electroporation knockout plasmid (pK18sB) to delete the C. necator megaplasmid copy of the CBB operon (deltaCBBp).

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for megaplasmid copy of the CBB operonthe CBB operon (deltaCBBp).
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    ∆cbbR ∆cbbLpSpXpYpEpFpPpTpZpGpKpAp

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pCHC024 (∆SH)

    Plasmid

    #226202

    Purpose

    Electroporation knockout plasmid (pK18sB) to delete the C. necator soluble hydrogenase operon (deltaSH).

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for soluble hydrogenase operon
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    ∆hoxFUYHWI ∆hypA2B2F2

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pCHC023 (∆MBH)

    Plasmid

    #226201

    Purpose

    Electroporation knockout plasmid (pK18sB) to delete the C. necator membrane-bound hydrogenase operon (deltaMBH).

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for membrane bound hydrogenase operon
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    ∆hoxKGZMLOQRTV ∆hypA1B1F1CDEX ∆hoxABCJ

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pCHC027 (∆pemK)

    Plasmid

    #226204

    Purpose

    Conjugation knockout plasmid (pK18msB) to delete the C. necator addiction system toxin PemK, to enable deletion of the pHG1 megaplasmid.

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Upstream/downstream homology arms for addiction system toxinPemK.
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 14, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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