We narrowed to 1,258 results for: grna cloning vector

1. pDGE510

   Plasmid

   #153282

   Purpose

   End-linker for cloning 4 sgRNA arrays into any recipient vector

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Insert

   linker
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-U6-CNCB

   Plasmid

   #236763

   Purpose

   A piggybac-based cloning vector containing mouse U6 promoter-driven sgRNA for spCas9 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

   Depositor

   Kazutoshi Takahashi

   Article

   Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

   Type

   Empty backbone

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6 promoter, CAG promoter

   Available since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCV_hU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-Thy1.1

   Plasmid

   #187925

   Purpose

   sgRNA vector backbone expressing puromycin resistance and CD90.1 as selection markers

   Depositor

   Martin Turner

   Article

   Turner et al eLife. 2022 Apr 22;11. pii: 72313. doi:

   Insert

   hU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-CD90.1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-UCOE-SFFV-dCas9-P2A-EGFP

   Plasmid

   #188898

   Purpose

   Human lentiviral vector for expression of dCas9 with a C-terminal HA-2xNLS and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and P2A-GFP

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPC1000

   Plasmid

   #174829

   Purpose

   Lentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 prime edit site

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Inserts

   CRISPRi SpCas9 sgRNA cassette
   PuroR-P2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Detailed in manuscript

   Promoter

   EF1a and mouse U6

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. MultiMate-HITI-2c ACTB

   Plasmid

   #206267

   Purpose

   All in one recombinant baculovirus production vector. Encodes Cas9, sgRNA and donor for homology independent targeted integration in the ACTB locus.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   SpCas9 (S. Pyogenes), hACTB HITI-2c donor, mCherry, Puro-R, hU6 hACTB sgRNA, AcrIIA4, eGFP
   

   Use

   CRISPR; Recombinant baculovirus production

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEIF3D-WT-sgNT

   Plasmid

   #222120

   Purpose

   EIF3D overexpression vector with non-targeting sgRNA

   Depositor

   Jonathan Weissman

   Article

   She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

   Insert

   eIF3D (EIF3D Human)
   

   Use

   Lentiviral

   Tags

   BFP-P2A

   Expression

   Mutation

   Promoter

   Available since

   March 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-Cas13X.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

   Plasmid

   #171379

   Purpose

   Expression vector for encoding a human codon-optimized Cas13X.1 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

   Depositor

   Hui Yang

   Article

   Xu et al Nat Methods. 2021 May;18(5):499-506. doi

   Insert

   humanized Cas13X.1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, U6

   Available since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-PGK-DIO-mCherry-nega miR

   Plasmid

   #137732

   Purpose

   Lentivirus vector for control miR in a Cre-dependent manner

   Depositor

   Tomoyuki Furuyashiki

   Article

   Nie et al Neuron. 2018 Aug 8;99(3):464-479.e7. doi

   Insert

   control microRNA
   

   Use

   Lentiviral

   Tags

   mChherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSIREN-RetroQ-HSPE-sh1

    Plasmid

    #92033

    Purpose

    pSIREN-RetroQ vector containing shRNA sequence to HPSE

    Depositor

    Joe Landry

    Article

    Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

    Insert

    shRNA #1 to HPSE (Hpse Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pSIREN-RetroQ-HSPE-sh2

    Plasmid

    #92034

    Purpose

    pSIREN-RetroQ vector containing shRNA sequence to HPSE

    Depositor

    Joe Landry

    Article

    Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

    Insert

    shRNA #2 to HPSE (Hpse Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. c3GIC9n

    Plasmid

    #62192

    Purpose

    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes

    Depositor

    Lukas Dow

    Article

    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

    Insert

    humanized S. Pyogenes Cas9 (D10A)
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mutation

    D10A substitution (Cas9n)

    Promoter

    TRE3G

    Available since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDGE Dicot Genome Editing Kit

    Plasmid Kit

    #1000000084

    Purpose

    Golden Gate cloning-based assembly of constructs for CRISPR genome editing in dicotyledonous plants. Consists of “genome editing recipient” and “sgRNA shuttle” vectors.

    Depositor

    Johannes Stuttmann

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pCW-IRF4-Puro

    Plasmid

    #123323

    Purpose

    Lentiviral vector for expression of IRF4 under a Doxycycline inducible promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

    Insert

    IRF4 (IRF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    several silent mutations to disrupt sgRNA targeti…

    Promoter

    Doxycycline inducible minimal CMV

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU6-(BsaI)_CBh-Cas9

    Plasmid

    #135010

    Purpose

    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9

    Depositor

    Yosef Shaul

    Article

    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

    Insert

    humanized S. pyogenes Cas9
    

    Use

    Tags

    3xFLAG (N terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEIF3D-RNAgateDel-sgEIF3D

    Plasmid

    #222137

    Purpose

    EIF3D variant overexpression vector with sgRNA targeting eIF3D

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    eIF3D (EIF3D Human)
    

    Use

    Lentiviral

    Tags

    BFP-P2A

    Expression

    Mutation

    V291-F306 deletion

    Promoter

    Available since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEIF3D-NtermDel-sgEIF3D

    Plasmid

    #222123

    Purpose

    EIF3D variant overexpression vector with sgRNA targeting eIF3D

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    eIF3D (EIF3D Human)
    

    Use

    Lentiviral

    Tags

    BFP-P2A

    Expression

    Mutation

    S161 truncation

    Promoter

    Available since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEIF3D-a11helixDel-sgEIF3D

    Plasmid

    #222135

    Purpose

    EIF3D variant overexpression vector with sgRNA targeting eIF3D

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    eIF3D (EIF3D Human)
    

    Use

    Lentiviral

    Tags

    BFP-P2A

    Expression

    Mutation

    T317E, N320E, H321A

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEIF3D-CtermDel-sgEIF3D

    Plasmid

    #222125

    Purpose

    EIF3D variant overexpression vector with sgRNA targeting eIF3D

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    eIF3D (EIF3D Human)
    

    Use

    Lentiviral

    Tags

    BFP-P2A

    Expression

    Mutation

    F527 truncation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEIF3D-a5helixDel-sgEIF3D

    Plasmid

    #222133

    Purpose

    EIF3D variant overexpression vector with sgRNA targeting eIF3D

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    eIF3D (EIF3D Human)
    

    Use

    Lentiviral

    Tags

    BFP-P2A

    Expression

    Mutation

    D249Q, V262I, Y263A

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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