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pCAG-MBP-ULK1-1-290-mcherrry

Plasmid

#249732

Purpose

Expressing human ULK1-1-290 with a MBP tag in N-terminal and a mcherry tag in C-terminal

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ULK1 (ULK1 Human)

Tags

MBP and Mcherry

Expression

Mammalian

Mutation

1-290

Promoter

CAG

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pIF1-GFP

Plasmid

#206925

Purpose

Plasmid expressing human IF1 fused in C-terminal with GFP

Depositor

José Cuezva

Article

Romero-Carraminana et al Commun Biol. 2023 Aug 12;6(1):836. doi:

Insert

human IF1 (ATPase inhibitory factor 1) (ATP5IF1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-BLAST-CMV-LiCre-WPRE (JDW 1495)

Plasmid

#242594

Purpose

Lentiviral vector with a CMV promoter driving a light inducible cre (LiCre).

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LiCre

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-Rps7-meGFP

Plasmid

#241318

Purpose

Mouse Rps7 fused to meGFP for expression in mammalian cells

Depositor

Stavros Lomvardas

Insert

Rps7 (Rps7 Mouse)

Tags

meGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 SLC31A1_sg2

Plasmid

#244870

Purpose

Knockout of human SLC31A1

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

SLC31A1 sgRNA (SLC31A1 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 SLC31A1_sg1

Plasmid

#244869

Purpose

Knockout of human SLC31A1

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

SLC31A1 sgRNA (SLC31A1 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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p3E-IRES-Luciferase-WPRE-bGH-pA (JDW 1509)

Plasmid

#242572

Purpose

Gateway 3' entry clone containing an IRES Luciferase

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

IRES-Luciferase-WPRE-bGH-pA

Use

Gateway Cloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pME-EGFP-miR-MCS (JDW 1231)

Plasmid

#242562

Purpose

Gateway middle entry clone containing an EGFP fused to a microRNA multiple cloning site.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EGFP with artifial intron and MCS

Use

Gateway Cloning

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pBabe-Hygro-RAS

Plasmid

#242639

Purpose

RAS expression

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

HRASG12V (HRAS Human)

Expression

Mammalian

Mutation

G12V

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AiP11580 - pAAV-hsA2-AiE0086m-minRho-SYFP2-WPRE3-BGHpA (Alias: CN1580)

Plasmid

#230099

Purpose

AiE0086m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minRho

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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AiP15291 - pAAV-AiE0710m-minBG-iCre(R297T)-BGHpA (Alias: CN5291)

Plasmid

#230510

Purpose

AiE0710m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minBG

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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AiP13787 - pAAV-AiE0140h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3787)

Plasmid

#230468

Purpose

AiE0140h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minBG

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Step-FLAG FBXO21

Plasmid

#236458

Purpose

transient overexpression of FBXO21 in mammalian cells

Depositor

Michele Pagano

Article

Donato et al Nat Cell Biol. 2017 Apr;19(4):341-351. d

Insert

FBXO21 (FBXO21 Human)

Tags

Strep-FLAG

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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AiP11598 - pAAV-hsA2-AiE0104m-minRho-SYFP2-WPRE3-BGHpA (Alias: CN1598)

Plasmid

#230178

Purpose

AiE0104m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minRho

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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AiP15285 - pAAV-AiE0170h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN5285)

Plasmid

#230509

Purpose

AiE0170h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minBG

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-hM3D(Gq)-mCherry-shLacZ-CWB

Plasmid

#223660

Purpose

Expresses hM3D(Gq) and a control shRNA (targetting LacZ) in a Cre-dependent manner

Depositor

Oscar Marin

Article

Selten et al Nature. 2025 Apr 30. doi: 10.1038/s41586

Insert

hM3D(Gq)-mCherry and shLacZ

Use

AAV and RNAi

Tags

mCherry

Expression

Mammalian

Promoter

hSyn

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-PDGFRA-V5/HIS

Plasmid

#234754

Purpose

Expression of human PDGFRA receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

PDGFRA receptor tyrosine kinase, full length, wildtype (PDGFRA Human)

Tags

V5/His

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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GB4080

Plasmid

#215249

Purpose

Module for the constitutive expression of EGFP, p19 and the N. nambi luminescence pathway genes fused to a SF.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

SF-EGFP-p19-LUZ-H3H-HISPS-CPH

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-DEST-Hsp70-zCreI-mTagBFP2d-2xins (JDW 1002)

Plasmid

#229817

Purpose

A gateway compatible Tol2 destination vector containing the Hsp70 promoter driving Cre and TagBFP followed by 2 cHS4 insulator cores.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

Tol2 destination vector

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12328 - pAAV-3xSP10ins-AiE0354h_3xC2-minRho-SYFP2-WPRE3-BGHpA (Alias: CN2328)

Plasmid

#230361

Purpose

AiE0354h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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