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pMRE151

Plasmid

#118501

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Expression

Bacterial

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pEY79

Plasmid

#191074

Purpose

flp-12 fluorescent neural reporter driving nuclear T-Sapphire expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-12 (flp-12 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEY79

Plasmid

#191074

Purpose

flp-12 fluorescent neural reporter driving nuclear T-Sapphire expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-12 (flp-12 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEY62

Plasmid

#191020

Purpose

acr-5 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

acr-5 (acr-5 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEY62

Plasmid

#191020

Purpose

acr-5 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

acr-5 (acr-5 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ2240 - [1-2] ENTR - Fluor - ce-tagRFP-T (1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159855

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-tagRFP-T (1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ2240 - [1-2] ENTR - Fluor - ce-tagRFP-T (1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159855

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-tagRFP-T (1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBstSK+IRES-V5-IPpoI

Plasmid

#206321

Purpose

A plasmid that facilitates production of mRNA encoding V5 epitope tagged I-PpoI

Depositor

Brian McStay

Article

van Sluis et al Genes Dev. 2015 Jun 1;29(11):1151-63. do

Insert

IRES element-V5 epitope tag- IPpoI open reading frame

Use

Mrna production

Tags

V5-IPpoI fusion protein

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBstSK+IRES-V5-IPpoI

Plasmid

#206321

Purpose

A plasmid that facilitates production of mRNA encoding V5 epitope tagged I-PpoI

Depositor

Brian McStay

Article

van Sluis et al Genes Dev. 2015 Jun 1;29(11):1151-63. do

Insert

IRES element-V5 epitope tag- IPpoI open reading frame

Use

Mrna production

Tags

V5-IPpoI fusion protein

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pMG

Plasmid

#138264

Purpose

Yeast intergration vector for integration of dual reporter system at the URA3 locus. Dual reporter system contains constitutive mCherry and constitutive eGFP flanked by iCas9 sites.

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Inserts

eGFP flanked by iCas9 sites
mCherry

Use

CRISPR

Expression

Yeast

Promoter

Tef1

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pMG

Plasmid

#138264

Purpose

Yeast intergration vector for integration of dual reporter system at the URA3 locus. Dual reporter system contains constitutive mCherry and constitutive eGFP flanked by iCas9 sites.

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Inserts

eGFP flanked by iCas9 sites
mCherry

Use

CRISPR

Expression

Yeast

Promoter

Tef1

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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R10-3 /pQE-30

Plasmid

#79859

Purpose

The first mutant of the Aequorea victoria green fluorescent protein that forms a red chromophore.

Depositor

Konstantin Lukyanov

Article

Mishin et al Biochemistry. 2008 Apr 22;47(16):4666-73

Insert

R10-3

Tags

HisTag

Expression

Bacterial

Mutation

Aequorea victoria green fluorescent protein (avGF…

Promoter

T5 promoter / lac operator

Available Since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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R10-3 /pQE-30

Plasmid

#79859

Purpose

The first mutant of the Aequorea victoria green fluorescent protein that forms a red chromophore.

Depositor

Konstantin Lukyanov

Article

Mishin et al Biochemistry. 2008 Apr 22;47(16):4666-73

Insert

R10-3

Tags

HisTag

Expression

Bacterial

Mutation

Aequorea victoria green fluorescent protein (avGF…

Promoter

T5 promoter / lac operator

Available Since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pEF-DEST51 HA-PNRC1

Plasmid

#123294

Purpose

Mammalian expression of PNRC1 N-HA

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

PNRC1 (PNRC1 Human)

Tags

HA

Expression

Mammalian

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pEF-DEST51 HA-PNRC1

Plasmid

#123294

Purpose

Mammalian expression of PNRC1 N-HA

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

PNRC1 (PNRC1 Human)

Tags

HA

Expression

Mammalian

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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deltaMyc-HA

Plasmid

#16556

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

c-MYC (MYC Human)

Tags

HA

Expression

Mammalian

Mutation

Deletion of aa 40-178

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
deltaMyc-HA

Plasmid

#16556

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

c-MYC (MYC Human)

Tags

HA

Expression

Mammalian

Mutation

Deletion of aa 40-178

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3 Flag Rab1a N124I

Plasmid

#46778

Purpose

expression of N124I Rab1a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab1a (Rab1a Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

N124I cannot stably bind guanine nucleotide

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3 Flag Rab1a N124I

Plasmid

#46778

Purpose

expression of N124I Rab1a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab1a (Rab1a Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

N124I cannot stably bind guanine nucleotide

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
IBMc071

Plasmid

#161943

Purpose

Contains Type IIS restriction site free elements for expressing protein to be split in IBM. ECF20 expression plasmid

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20-T

Expression

Bacterial

Promoter

PBAD

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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