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pCVD404

Plasmid

#66149

Purpose

to make a probe to detect Heat stable enterotoxin gene (porcine)

Depositor

James Kaper

Publication

Nishibuchi et al J Clin Microbiol. 1988 Apr;26(4):784-6.

Insert

STp

Use

Cloning vector

Promoter

none

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pGL3-PDE6B-146

Plasmid

#239098

Purpose

pGL3 luciferase vector with 146bp PDE6B promoter (containing NRL response element).

Depositor

David Cobrinik

Publication

Shayler et al eLife 13:RP101918

Insert

PDE6B promoter (PDE6B Human)

Use

Luciferase

Available since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRSF-coSnaA-TEV-His_His-TEV-SnaC

Plasmid

#225059

Purpose

recombinant expression of SnaA in E. coli

Depositor

Wilfred van der Donk

Publication

Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

Insert

SnaA

Expression

Bacterial

Promoter

T7

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pet15b _yein

Plasmid

#220226

Purpose

Bacterial expression of YeiN in E. coli BL21_DE3 (pLys) or similar

Depositor

Bernd Nidetzky

Insert

psug (psuG E.coli)

Tags

Histag

Expression

Bacterial

Promoter

T7

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pMDC123-SUVH9-ZF

Plasmid

#200923

Purpose

Express SUVH9-ZF108 in Arabidopsis target FWA gene

Depositor

Steven Jacobsen

Publication

Wang et al Nat Plants. 2023 Mar 6. doi: 10.1038/s41

Insert

SUVH9 (SUVH9 Mustard Weed)

Expression

Plant

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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SF3B3

Plasmid

#156003

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SF3B3 (SF3B3 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

S6L

Promoter

EF-1a

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pUC_hU6-shRKIP_BFP

Plasmid

#199216

Purpose

Expression plasmid with shRNAmiR targeting RKIP (PEBP1), with a BFP reporter on the backbone.

Depositor

Gabor Balazsi

Publication

Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

Insert

shBACH1 (BACH1 Synthetic)

Use

RNAi

Promoter

human U6

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (E60amb)-His

Plasmid

#168043

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding E60 changed to amber codon (TAG).

Depositor

Michael Nash

Publication

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Glu 60 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (S87amb)-His

Plasmid

#168044

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding S87 changed to amber codon (TAG).

Depositor

Michael Nash

Publication

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Ser 87 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (N21amb)-His

Plasmid

#168041

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding N21 changed to amber codon (TAG).

Depositor

Michael Nash

Publication

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Asn 21 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (I55amb)-His

Plasmid

#168042

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding I55 changed to amber codon (TAG).

Depositor

Michael Nash

Publication

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Ile 55 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pBA cMYC-AIP2

Plasmid

#118667

Purpose

Plant overexpression

Depositor

Kristiina Himanen

Publication

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

AIP2 (AT5G20910 Mustard Weed)

Tags

Myc

Expression

Plant

Promoter

CaMV35S

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pZA17

Plasmid

#158495

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1. Km plant selection marker.

Depositor

Sophien Kamoun

Publication

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Expression

Plant

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pSB819-PKR-tam

Plasmid

#20036

Depositor

Harmit Malik

Publication

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R

Expression

Yeast

Available since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA4-C-EF1α-FL-NRL

Plasmid

#239095

Purpose

Expresses full length NRL, driven by EF1α promoter.

Depositor

David Cobrinik

Publication

Shayler et al eLife 13:RP101918

Insert

Neural Retina Leucine Zipper (NRL Human)

Expression

Mammalian

Available since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p35S-FHA-SpAGO4a_gDNA

Plasmid

#216841

Purpose

N-term Flag-HA tagged Spirodela polyrhiza (Sp9509) AGO4a gDNA under regulation of CaMV 35S promoter

Depositor

Arturo Marí-Ordóñez

Publication

Dombey et al Genome Res. 2025 Mar 18;35(3):522-544. d

Insert

ARGONAUTE4

Tags

Flag-HA

Expression

Plant

Promoter

CaMV 35S

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p35S-FHA-SpAGO4a_cDNA

Plasmid

#216842

Purpose

N-term Flag-HA tagged Spirodela polyrhiza (Sp9509) AGO4a cDNA under regulation of CaMV 35S promoter

Depositor

Arturo Marí-Ordóñez

Publication

Dombey et al Genome Res. 2025 Mar 18;35(3):522-544. d

Insert

ARGONAUTE4

Tags

Flag-HA

Expression

Plant

Promoter

CaMV 35S

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEG302-CPL2-ZF

Plasmid

#200918

Purpose

Express CPL2-ZF108 in Arabidopsis target FWA gene

Depositor

Steven Jacobsen

Publication

Wang et al Nat Plants. 2023 Mar 6. doi: 10.1038/s41

Insert

CPL2 (CPL2 Mustard Weed)

Expression

Plant

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-NDUFA10-FKBP-HA

Plasmid

#185810

Purpose

NDUFA10 subunit of Complex I fused with C-terminal FKBP and HA epitope

Depositor

Dipayan Chaudhuri

Publication

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFA10 (NDUFA10 Human)

Use

Lentiviral

Tags

FKBP and HA

Expression

Mammalian

Promoter

CMV

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pTF101.1gw3

Plasmid

#172181

Purpose

A binary plasmid for gateway 3-Entry in pTF101.1 background (attR1 & attR2) containing double CaMV 35S promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.

Depositor

Kan Wang

Publication

Paz et al Plant Cell Rep. 2006 Mar;25(3):206-13. d

Insert

attR3-CmR-ccdB-attR4

Expression

Plant

Promoter

lacUV5 promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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